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Volume 12 Issue 1
Apr.  2012
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Article Contents
wang Mingxia, Chen Jingying. Comparision Between Two Nonradioactive Labelled DNA Probe for the Detection of Chrysanthemum Stund Viroid[J]. Fujian Journal of Agricultural Sciences, 1997, 12(1): 56-59.
Citation: wang Mingxia, Chen Jingying. Comparision Between Two Nonradioactive Labelled DNA Probe for the Detection of Chrysanthemum Stund Viroid[J]. Fujian Journal of Agricultural Sciences, 1997, 12(1): 56-59.

Comparision Between Two Nonradioactive Labelled DNA Probe for the Detection of Chrysanthemum Stund Viroid

  • Received Date: 1995-09-29
  • Publish Date: 1997-03-15
  • Recombinant PUC inserted with full length nucleic acid sequence of chrysanthemum stunt viroid was used as template DNA to prepare digoxigenin-labelled and biotin-labelled probes with two universal primers by polymerase chain reaction.These two Probes were used to detect Samples infected with CSV.The result showed strong positive signals, while the control of the healthy ones gave negative reaction.Absorbance of digoxigenin-labelled probe was higher than that of biotin-labelled Probe for detecting CSV cDNA with PCR-microplate hybridization.The sensitivity of the digoxigenin-fataled probe for detecting the CSV RNA by dot blot method was 50fg, while that of the biotin-labelled Probe for detectingthe CSV RNA was 5pg.
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  • [3] M.Welnicki and C.Hiruki Highly sensitive digoxigenin-labelled DNA probe for the detection of potato spinder tuber virroid.Journal of Virological Methods.1992,39:91-99
    [5] Tatsuji Hataya,Alice Kazuko moue and Eishiro Shikata.A PCR-microplate hybridization method for plant virus detection.journal of Virological Methods,1993,46:223-236
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