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Volume 13 Issue 1
Apr.  2012
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Tian Yanan, Ke Sui, Ke Chong. The Progress on Diagnosis and Detection of Citrus Huanglongbing[J]. Fujian Journal of Agricultural Sciences, 1998, 13(1): 27-35.
Citation: Tian Yanan, Ke Sui, Ke Chong. The Progress on Diagnosis and Detection of Citrus Huanglongbing[J]. Fujian Journal of Agricultural Sciences, 1998, 13(1): 27-35.

The Progress on Diagnosis and Detection of Citrus Huanglongbing

  • Received Date: 1997-10-16
  • Publish Date: 1998-03-15
  • Diagnosis and detection are important aspect of citrus Huanglongbing research In the past,diagnosis of citrus Huanglongbing was mainly based on its sympomatology,namely,yellow shoot and mottle leaf During 1977-1978,the causative bacterium like organism (BLO) was found in sieve cells of diseased leaf under electron microscope After then,the electron microscope has been used as a major tool for diagnosis of citrus Huanglongbing disease An improvement came about when it was found that the BLO can be transmitted from citrus to periwinkle by dodder and partially purified by differential centrifugation,which made it possible to prepare antisera,both monoclonal and polyclonal antibodies,for detection and diagnosis of Huanglongbing BLO Recently,polymerase chain reaction (PCR) has been developed for detection and quanfitication of Huanglongbing BLO The PCR-based methods are highly sensitive and effective in detecting the presence of the BLO in diseased plant and insect vectors carrying the BLO Furthermore the BLO in the samples can be quantitated by quantitative PCR making it possible to compare the amounts of BLO in different samples.
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    Villechanoux S,et al.T he genom e of the Non-cultured,bact eria-like or ganis massociated with citras greening disease contains the nusg-rpl K A J L-rpoBe gene cluster and the gene for a bact erioph age type DN Apolymerase.Current Microbiology,1993,26:161-166
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    田亚南,柯穗,李韬,等.应用电镜与PCR技术检测溪蜜柚黄龙病病原.植物病理学报(在印刷中)
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