Transcriptome analysis of genetic differences between aerial and strandy hyphae of <i>Agaricus bisporus</i> strain As2796

DAI Jianqing; CHEN Wenzhi; ZENG Zhiheng; CAI Zhixin; CHEN Meiyuan

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DAI J Q, CHEN W Z, ZENG Z H, et al. Transcriptome analysis of genetic differences between aerial and strandy hyphae of Agaricus bisporus strain As2796 [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−12

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DAI J Q, CHEN W Z, ZENG Z H, et al. Transcriptome analysis of genetic differences between aerial and strandy hyphae of Agaricus bisporus strain As2796 [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−12

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Transcriptome analysis of genetic differences between aerial and strandy hyphae of Agaricus bisporus strain As2796

The Edible mushroom Institute of Fujian Academy of Agricultural Sciences, National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible mushroom, Fuzhou　350013, Fujian, China

Received Date: 2024-08-09
Rev Recd Date: 2024-09-01

Available Online: 2024-10-31

Abstract

Abstract

Objective Excavating the important genes that may be involved in the morphological changes of Agaricus bisporus colony can provide scientific basis for the evaluation of germplasm resources and the breeding of new varieties. Methods As2796, the main cultivated variety of Agaricus bisporus, was used as the test strain. The strandy and aerial hyphae grown on the same plate were collected. RNA of the samples was extracted for transcriptome sequencing and compared with the reference genome sequence of Agaricus bisporus. Results Through transcriptome analysis, a total of 965 differentially expressed genes (DEGs) were identified in A601 (As2796 aerial group) and A602 (As2796 strandy group). GO enrichment analysis results showed that hydrophobin-related DEGs were enriched in fungal cell wall composition. Among them, AGABI2DRAFT_13655, AGABI2DRAFT_136569, and AGABI2DRAFT_193057 belong to the ABH3 protein gene family. The expression level in aerial hyphae is significantly higher thanstrandy hyphae, which may be related to the production of aerial hyphae. The study also found that the hydrophobic surface binding protein A (HsbA)-related gene AGABI2DRAFT_194662 ,the FPKM expression level of this gene is 0 in aerial hyphae and 10.24 in strandy hyphae. Through further analysis of DEGs, 8 DEGs that may be related to lipase isoenzymes were enriched. The FPKM expression levels of these genes in aerial hyphae were significantly higher than strandy hyphae. The SNP/InDel analysis results showed that the number of SNPs in the As2796 aerial group was 110601, in strandy group was 111188. The main changes in single nucleotides were C:G＞T:A, and a total of 2374 common InDels were screened. Conclusion This study successfully obtained transcriptome data on mycelial morphological changes in Agaricus bisporus and unearthed potential related genes and SNP sites, which provides a reference for the evaluation of Agaricus bisporus germplasm resources and the breeding of new varieties..
- Agaricus bisporus,
- As2796,
- Strandy hypha,
- Aerial hypha,
- Transcriptome sequencing,
- Differentially expressed genes (DEGs)

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References

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