Endophytic and Rhizosphere Microbes in <i>Pinellia ternata</i> and Habitat Soils in Guizhou Affected by Environmental Conditions

CHEN Xiaofang; HU Tianji; ZHANG Xiangyu; REN Xiyi; LIU Min

doi:10.19303/j.issn.1008-0384.2024.07.010

Volume 39 Issue 7

Jul. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(7): 826-838

CHEN X F, HU T J, ZHANG X Y, et al. Endophytic and Rhizosphere Microbes in Pinellia ternata and Habitat Soils in Guizhou Affected by Environmental Conditions [J]. Fujian Journal of Agricultural Sciences，2024，39（7）：826−838 doi: 10.19303/j.issn.1008-0384.2024.07.010

Citation:

CHEN X F, HU T J, ZHANG X Y, et al. Endophytic and Rhizosphere Microbes in Pinellia ternata and Habitat Soils in Guizhou Affected by Environmental Conditions [J]. Fujian Journal of Agricultural Sciences，2024，39（7）：826−838 doi: 10.19303/j.issn.1008-0384.2024.07.010

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Endophytic and Rhizosphere Microbes in Pinellia ternata and Habitat Soils in Guizhou Affected by Environmental Conditions

doi: 10.19303/j.issn.1008-0384.2024.07.010

1.
Bijie Medical College, Bijie, Guizhou　551700, China
2.
Guizhou Zhongjianlian Biology Science and Technology Co., Ltd., Bijie, Guizhou　551700, China
3.
Bijie Institute of Traditional Chinese Medicine, Bijie, Guizhou　551700, China
4.
Biotechnology Institute of Guizhou Province, Guiyang, Guizhou　550006, China

Received Date: 2023-11-01
Rev Recd Date: 2024-04-30

Available Online: 2024-08-15

Publish Date: 2024-07-01

Abstract

Abstract

Objective Endophytic microbes of Pinellia ternata and rhizosphere microbial communities in the habitat soils in Guizhou as affected by the environmental conditions were analyzed. Methods High throughput sequencing to identify the endophytic and rhizosphere microbiota in P. ternata and soils of the wild, large-scale cultivated, and intercropped P. ternata plants grew in Guizhou was conducted. Microbial diversity, species, relationships, and enzyme activities as well as functional microflora were analyzed. Bioinformatics was employed to decipher the ecological relationship between the plant and its habitat. Results The environmental, physiochemical, and enzymatic conditions on the land where P. ternata plants were grown in the wild or under cultivation varied significantly. For instance, the organic matter content and acidity were high in the cultivated plots, but the enzyme activity was high in the soil of virgin forest. In the plants, Rhizobium, Pseudomonas, Bacillus, and Bradyrhizobium were the dominant endophytic bacteria genera, whereas Campylospora, Neocosmospora,Rhizoctonia, Fusarium, Dactylonectria, unclassified genera of Xylariales order, etc. were found to be the dominant fungi genera. In the rhizosphere soil, abundant dominant microbial species regularly aggregated and were affected by the environmental factors and planting method. For example, 10 dominant bacteria genera and 13 fungi genera significantly correlated with the physicochemical properties and/or enzyme activity of the soil they inhabited. In general, the endophytic microbes in the plants were more sensitive to the environmental factors than those in the rhizosphere soil. And they could be synergistic as well as antagonistic to one another, but the rhizosphere community tended to be stable. Aside from the unclassified and unknown genera, the dominant endophytic bacteria were mostly considered beneficial for the plant. Conclusion The microbial community in the soil could be improved by adjusting pH and applying microbial fertilizers. The endophytic microbes of P. ternate including Rhizobia,Pseudomonas, Bacillus, Agrobacterium, Trichoderma, and Metarhizium anisopliae could be used to enhance plant growth ushering in the development of a pharmaceutical industry based on the medicinal material.
- Pinellia ternata,
- endophyte,
- soil microbes,
- environmental factor

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References(20)

References

[1]	陈晓芳, 张翔宇, 柳敏, 等. 贵州半夏叶褐斑病病原菌鉴定及其防治药剂室内毒力测定 [J]. 中药材, 2023, 46(5):1094−1099. CHEN X F, ZHANG X Y, LIU M, et al. Identification of pathogen causing leaf brown spot of Pinellia ternata in Guizhou and indoor toxicity test of its control chemicals [J]. Journal of Chinese Medicinal Materials, 2023, 46(5): 1094−1099. (in Chinese)
[2]	许佳伟, 罗鸣, 徐荣, 等. 中国半夏主产地软腐病致病菌分离、鉴定及比较研究 [J]. 西南农业学报, 2023, 36(9):1950−1961. XU J W, LUO M, XU R, et al. Isolation, identification and comparative study of soft rot pathogens of Pinellia ternata in China [J]. Southwest China Journal of Agricultural Sciences, 2023, 36(9): 1950−1961. (in Chinese)
[3]	赵玳琳, 何海永, 谭清群, 等. 贵州省赫章县半夏软腐病病原鉴定 [J]. 中药材, 2022, 45(2):278−283. ZHAO D L, HE H Y, TAN Q Q, et al. Identification of pathogens of Pinellia ternatea soft rot in Hezhang County of Guizhou Province [J]. Journal of Chinese Medicinal Materials, 2022, 45(2): 278−283. (in Chinese)
[4]	HE Z G, MAO R J, DONG J E, et al. Remediation of deterioration in microbial structure in continuous Pinellia ternata cropping soil by crop rotation [J]. Canadian Journal of Microbiology, 2019, 65(4): 282−295. doi: 10.1139/cjm-2018-0409
[5]	何冬梅, 赖长江生, 严铸云, 等. 中药微生态研究与展望 [J]. 中国中药杂志, 2018, 43(17):3417−3430. HE D M, LAI C J S, YAN Z Y, et al. Research and prospect of Traditional Chinese Medical Microecology [J]. China Journal of Chinese Materia Medica, 2018, 43(17): 3417−3430. (in Chinese)
[6]	王红阳, 康传志, 王月枫, 等. 药用植物微生物组的研究现状及展望 [J]. 中国中药杂志, 2022, 47(20):5397−5405. WANG H Y, KANG C Z, WANG Y F, et al. Medicinal plant microbiome: Advances and prospects [J]. China Journal of Chinese Materia Medica, 2022, 47(20): 5397−5405. (in Chinese)
[7]	吕佩, 王新绘, 刘晓颖, 等. 药用植物刺山柑不同部位细菌群落结构及其多样性 [J]. 微生物学报, 2023, 63(10):3939−3954. LÜ P, WANG X H, LIU X Y, et al. Bacterial diversity and communities in different parts of the medicinal plant Capparis spinosa L [J]. Acta Microbiologica Sinica, 2023, 63(10): 3939−3954. (in Chinese)
[8]	王礼科, 罗夫来, 王华磊, 等. 半夏不同连作年限土壤酶活性、微生物及化感物质的分析 [J]. 中药材, 2021, 44(4):798−801. WANG L K, LUO F L, WANG H L, et al. Analysis of soil enzyme activities, microorganisms and allelochemicals in different continuous cropping years of Pinellia ternata [J]. Journal of Chinese Medicinal Materials, 2021, 44(4): 798−801. (in Chinese)
[9]	刘诗蓉, 王红兰, 孙辉, 等. 半夏连作对根际土壤微生物群落的影响研究 [J]. 中草药, 2022, 53(4):1148−1155. LIU S R, WANG H L, SUN H, et al. Effects of continuous cropping of Pinellia ternata on rhizospheric microbial community [J]. Chinese Traditional and Herbal Drugs, 2022, 53(4): 1148−1155. (in Chinese)
[10]	陈晓芳, 张翔宇, 柳敏, 等. 根腐病半夏内生菌群及其土壤微生物群落分析 [J]. 中药材, 2023, 46(10):2399−2407. CHEN X F, ZHANG X Y, LIU M, et al. Analysis of endophytic flora and soil microbial community of Pinellia ternata with root rot [J]. Journal of Chinese Medicinal Materials, 2023, 46(10): 2399−2407. (in Chinese)
[11]	MISHRA S, GOYAL D, PHURAILATPAM L. Targeted 16S rRNA gene and ITS2 amplicon sequencing of leaf and spike tissues of Piper longum identifies new candidates for bioprospecting of bioactive compounds [J]. Archives of Microbiology, 2021, 203(7): 3851−3867. doi: 10.1007/s00203-021-02356-w
[12]	张翔宇, 陈晓芳, 柳敏, 等. 不同栽培模式重楼内生真菌、根际真菌多样性与环境因子关联性研究[J/OL]. 中药材, 2023(6): 1353-1360. ZAHNG X Y, CHEN X F, LIU M, et al. Study on the Diversity of Endophytic Fungi and Rhizosphere Fungi in Different Cultivation Models and their Correlation with Environmental Factors of Paris polyphylla[J/OL]. ProvinceJournal of Chinese Medicinal Materials, 2023(6): 1353-1360. (in Chinese)
[13]	秦杰, 高振峰, 岳爱琴, 等. 一株晋大53号大豆中慢生根瘤菌的分离鉴定及抗逆分析 [J]. 大豆科学, 2020, 39(6):898−905. doi: 10.11861/j.issn.1000-9841.2020.06.0898 QIN J, GAO Z F, YUE A Q, et al. Isolation, identification and stress resistance analysis of A Mesorhizobium isolated from soybean variety jinda 53 [J]. Soybean Science, 2020, 39(6): 898−905. (in Chinese) doi: 10.11861/j.issn.1000-9841.2020.06.0898
[14]	马福林, 仁增卓玛, 王昌玲, 等. 西藏沙棘根瘤内生假单胞菌的分离鉴定及促生性研究 [J]. 福建农业学报, 2023, 38(5):624−631. MA F L, RENZENG Z M, WANG C L, et al. Identification and Growth-promoting Effects of Endophytic Pseudomonas sp. From Hippophae thibetana Root Nodules [J]. Fujian Journal of Agricultural Sciences, 2023, 38(5): 624−631. (in Chinese)
[15]	刘艳丽, 赵娜娜, 范贝贝, 等. 浓缩沼液中添加芽孢杆菌对促生和抑菌功能的强化效应 [J]. 中国农业大学学报, 2023, 28(7):68−78. doi: 10.11841/j.issn.1007-4333.2023.07.06 LIU Y L, ZHAO N N, FAN B B, et al. Enhancement effect of Bacillus spp. added to concentrated biagas slurry on the functions of growth promotion and bacterial inhibition [J]. Journal of China Agricultural University, 2023, 28(7): 68−78. (in Chinese) doi: 10.11841/j.issn.1007-4333.2023.07.06
[16]	施河丽, 向必坤, 彭五星, 等. 有机无机肥料配施对植烟土壤养分及细菌群落结构的影响 [J]. 中国土壤与肥料, 2019, (4):58−66. doi: 10.11838/sfsc.1673-6257.18352 SHI H L, XIANG B K, PENG W X, et al. Effects of combined application of organic and inorganic fertilizers on flue-cured tobacco soil nutrients and bacterial community structure [J]. Soil and Fertilizer Sciences in China, 2019(4): 58−66. (in Chinese) doi: 10.11838/sfsc.1673-6257.18352
[17]	石义妃, 耿佩冰, 吴皓, 等. 金黄垂直链霉菌DF06的分类鉴定及防病促生作用 [J]. 中国生物防治学报, 2023, 39(2):407−417. SHI Y F, GENG P B, WU H, et al. Classification and identification of Streptomyces aureoverticillatus DF06 and its effect on disease control and growth promotion [J]. Chinese Journal of Biological Control, 2023, 39(2): 407−417. (in Chinese)
[18]	刘辉, 韦璐璐, 朱龙发, 等. 鞘氨醇单胞菌的研究进展 [J]. 微生物学通报, 2023, 50(6):2738−2752. LIU H, WEI L L, ZHU L F, et al. Research progress of Sphingomonas [J]. Microbiology China, 2023, 50(6): 2738−2752. (in Chinese)
[19]	KHAN A A R. 木霉属次生代谢提取物对植物病原真菌、细菌和线虫的活性筛选研究[D]. 北京: 中国农业科学院, 2021. KHAN A A R . Screening and Antimicrobial Efficacy of Secondary Metabolites from Trichoderma species Against Plant Pathogenic Fungus, Bacteria and Nematode[D]. Beijing: Chinese Academy of Agricultural Sciences, 2021. (in Chinese)
[20]	农向群, 王广君, 蔡霓, 等. 绿僵菌与植物的多重关系及其在植物保护中的应用潜力 [J]. 植物保护, 2022, 48(3):22−30,54. NONG X Q, WANG G J, CAI N, et al. Multiple association of Metarhizium with plants and the application potential in plant protection [J]. Plant Protection, 2022, 48(3): 22−30,54. (in Chinese)