Cloning and Preliminary Functional Verification of <i>VfNHX1</i> in <i>Vicia faba</i> L.

JIN Wenhai; FAN Youcun; LI Ping; FAN Huiling; HOU Wanwei; TENG Changcai; LIU Yujiao; WU Xuexia

doi:10.19303/j.issn.1008-0384.2024.06.008

Volume 39 Issue 6

Jun. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(6): 689-699

JIN W H, FAN Y C, LI P, et al. Cloning and Preliminary Functional Verification of VfNHX1 in Vicia faba L. [J]. Fujian Journal of Agricultural Sciences，2024，39（6）：689−699 doi: 10.19303/j.issn.1008-0384.2024.06.008

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JIN W H, FAN Y C, LI P, et al. Cloning and Preliminary Functional Verification of VfNHX1 in Vicia faba L. [J]. Fujian Journal of Agricultural Sciences，2024，39（6）：689−699 doi: 10.19303/j.issn.1008-0384.2024.06.008

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Cloning and Preliminary Functional Verification of VfNHX1 in Vicia faba L.

doi: 10.19303/j.issn.1008-0384.2024.06.008

JIN Wenhai^{1, 2
,},
FAN Youcun^{1, 2},
LI Ping³,
FAN Huiling⁴,
HOU Wanwei³,
TENG Changcai³,
LIU Yujiao^{1, 3},
WU Xuexia^{1, 5
,
,}

1.
Key Laboratory of Plateau Ecology of the Province and Qinghai Agriculture University, Xining, Qinghai　810016, China
2.
College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai　810016, China
3.
Academy of Agricultural and Forestry, Qinghai University, Xining, Qinghai　810016, China
4.
College of Agriculture and Biotechnology, Hexi University, Zhangye, Gansu　734099, China
5.
Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining, Qinghai　810016, China

Received Date: 2024-01-03
Accepted Date: 2024-06-02
Rev Recd Date: 2024-05-28

Available Online: 2024-07-10

Publish Date: 2024-06-28

Abstract

Abstract

Objective Role of VfNHX1 of faba bean in response to salt stress was studied. Methods A Na⁺/H⁺ reverse transporter protein-encoding gene, VfNHX1, was cloned from Vicia faba L. by 3' and 5' RACE for a bioinformatics analysis, subcellular localization determination, expression under salt stress, and a preliminary verification on the function. Results (1) VfNHX1 sequence was 2255 bp with a CDS coding region of 1629 bp encoding 542 amino acids. (2) As a stable transmembrane protein, VfNHX1 had 10 transmembrane regions without signal peptide and contained a typical Na-H exchanger conserved functional domain of NHX family. It was in the vacuolar membrane. (3) In the leaves, the expression of VfNHX1 under NaCl stress determined by qRT-PCR showed a trend of decreasing, then increasing, followed by decreasing with a peak reached in 12 h. In the roots, the expression declined initially and then rose significantly at 48 h (P＜0.01). (4) The presence of VfNHX1 in a yeast growth experiment indeed heightened the NaCl tolerance of the salt-sensitive yeast mutant AXT4K. Conclusion VfNHX1 in V. faba L. was able to respond to salt stress and could be a functional gene to boost the salt resistance of the plant.
- Vicia faba L.,
- Na⁺/H⁺ antiporter,
- cloning,
- salt stress,
- functional verification

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References(32)

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