Physiobiochemical Changes and Gene Expressions of <i>Polygonatum</i> <i>cyrtonema</i> Hua Seeds undergone Post-ripening Treatment

SU Hailan; NIU Yuqing; CHENG Jianhua; ZHU Yanming; CHEN Hong; ZHENG Meixia; HUANG Xiangui; ZHU Yujing

doi:10.19303/j.issn.1008-0384.2024.06.005

Volume 39 Issue 6

Jun. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(6): 662-670

SU H L, NIU Y Q, CHENG J H, et al. Physiobiochemical Changes and Gene Expressions of Polygonatum cyrtonema Hua Seeds undergone Post-ripening Treatment [J]. Fujian Journal of Agricultural Sciences，2024，39（6）：662−670 doi: 10.19303/j.issn.1008-0384.2024.06.005

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SU H L, NIU Y Q, CHENG J H, et al. Physiobiochemical Changes and Gene Expressions of Polygonatum cyrtonema Hua Seeds undergone Post-ripening Treatment [J]. Fujian Journal of Agricultural Sciences，2024，39（6）：662−670 doi: 10.19303/j.issn.1008-0384.2024.06.005

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Physiobiochemical Changes and Gene Expressions of Polygonatum cyrtonema Hua Seeds undergone Post-ripening Treatment

doi: 10.19303/j.issn.1008-0384.2024.06.005

1.
Institute of Crop Sciences, Fujian Academy of Agricultural Sciences/Fujian Germplasm Resources Center, Fuzhou, Fujian　350003, China
2.
Shaowu Forest Bureau, Nanping, Fujian　354000, China
3.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350012, China

Received Date: 2024-01-11
Rev Recd Date: 2024-03-18

Available Online: 2024-06-26

Publish Date: 2024-06-28

Abstract

Abstract

Objective Physiobiochemical changes and differentially expressed genes in Polygonatumcyrtonema Hua seeds after a post-ripening treatment were studied. Method Contents of superoxide dismutase, α-amylase, β-amylase, soluble protein, crude fat, and soluble sugar at 6 seed development stages were monitored. Differentially expressed genes were analyzed with high-throughput sequencing followed by a qRT-PCR verification. Result (1) The post-ripening treatment raised the soluble sugar content in the seeds gradually in the initial 4 stages and slightly declined in the last two stages, while induced a declining trend on the crude fat and protein. Meanwhile, the activities of α-amylase and β-amylase increased. (2) Significantly enriched differentially expressed genes were observed in the sucrose and starch metabolic pathways as well as the hormone signal transduction pathway during the process. Conclusion The post-ripening treatment on the P. cyrtonema seeds brought about the conversion and metabolism of starch and other nutrients as well as endogenous hormones interactions promoting the physiological changes and dormancy release.
- Polygonatum cyrtonema Hua,
- physiological post-ripening,
- transcriptome sequencing analysis

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References(29)

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