Isolation, Identification, Biological Characteristics, and Fungicide Toxicity of Atractylodes Root Rot Pathogen

GU Qingyi; ZHANG Yaozhou; WU Xiaoya; HUANG Yaqin; QIAO Xinrong; SHEN Jun

doi:10.19303/j.issn.1008-0384.2024.03.010

Volume 39 Issue 3

Mar. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(3): 330-338

GU Q Y, ZHANG Y Z, WU X Y, et al. Isolation, Identification, Biological Characteristics, and Fungicide Toxicity of Atractylodes Root Rot Pathogen [J]. Fujian Journal of Agricultural Sciences，2024，39（3）：330−338 doi: 10.19303/j.issn.1008-0384.2024.03.010

Citation:

GU Q Y, ZHANG Y Z, WU X Y, et al. Isolation, Identification, Biological Characteristics, and Fungicide Toxicity of Atractylodes Root Rot Pathogen [J]. Fujian Journal of Agricultural Sciences，2024，39（3）：330−338 doi: 10.19303/j.issn.1008-0384.2024.03.010

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Isolation, Identification, Biological Characteristics, and Fungicide Toxicity of Atractylodes Root Rot Pathogen

doi: 10.19303/j.issn.1008-0384.2024.03.010

School of Pharmaceutical School of Pharmacy, Xinyang Agriculture and Forestry University, Xinyang , Henan 464000, China

Received Date: 2023-10-25
Rev Recd Date: 2024-01-12

Available Online: 2024-03-28

Publish Date: 2024-03-28

Abstract

Abstract

Objective Pathogen that caused the root rot on Atractylodes macrocephala at Xinyang, Henan Province was isolated, identified, and studied for prevention and control of the disease. Method Morphological and multiple gene analyses were employed to identify the pathogen. Biological characteristics of the isolate were determined. Result The isolate had white hyphae, oval spores with slightly pointed ends, macroconidia sized (7–10) μm× (3–4) μm with 3–5 septa, and microconidia sized (3–5) μm × (1–2) μm with two or no compartments. It showed a high homology with Fusarium fujikuroi and was, for the first time, identified as the fungal pathogen responsible for the root rot disease on Atractylodes macrocephala. It thrived at 28 ℃, pH 7, sucrose for carbon, and potassium nitrate for nitrogen. Of the 3 plant-based fungicides tested, 0.3% eugenol soluble agent exerted the greatest toxicity on the isolate with an EC₅₀ of 6.906 mg·L⁻¹. Conclusion F. fuciformis was identified as the pathogen that caused the root rot disease on A. macrocephala in Henan. The optimal culture conditions for the fungus included 28 ℃, pH 7, sucrose as the carbon source, and potassium nitrate as the nitrogen source.
- Atractylodes macrocephala,
- root rot disease,
- Fusarium fujikura,
- botanical fungicides

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References

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