Identification and Expressions of <i>YUCCA</i> Family in <i>Passiflora edulis</i>

PAN Jiayi; PAN Ruoyun; JIANG Wenjie; REN Rui; FANG Ting

doi:10.19303/j.issn.1008-0384.2024.02.006

Volume 39 Issue 2

Feb. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(2): 165-174

PAN J Y, PAN R Y, JIANG W J, et al. Identification and Expressions of YUCCA Family in Passiflora edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：165−174 doi: 10.19303/j.issn.1008-0384.2024.02.006

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PAN J Y, PAN R Y, JIANG W J, et al. Identification and Expressions of YUCCA Family in Passiflora edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：165−174 doi: 10.19303/j.issn.1008-0384.2024.02.006

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Identification and Expressions of YUCCA Family in Passiflora edulis

doi: 10.19303/j.issn.1008-0384.2024.02.006

College of Horticulture, Fujian Agriculture and Forestry Universituy/Institute of Horticultural Plant Genetics and Breeding, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2023-09-11
Rev Recd Date: 2023-11-13

Available Online: 2024-03-28

Publish Date: 2024-02-28

Abstract

Abstract

Objective Bioinformatics of YUCCA family encoding the flavin-containing monooxygenase associated with biosynthesis of indole-3-acetic acid (IAA) in passion fruit was studied. Methods Bioinformatic methods were applied to analyze the physicochemical properties, conserved domains, chromosome location, structure, phylogenetic tree, and cis-acting elements of the genes in Passiflora edulis Sims. qRT-PCR was used to determine the expressions of some members under IAA treatment. Results There were 29 YUCCA members unevenly distributed in 8 chromosomes of P. edulis. They significantly differed in length that ranged from 552 bp to 9210 bp and contained 1–8 introns and 8 conserved motifs. A phylogenetic tree analysis divided the family into three distinct categories, and within a same class the members were highly conservative. Genetically, the genes were more closely related to Medicago sativa L. and Arabidopsis thaliana than Oryza sativa L. The cis-acting element analysis indicated that the promoter of the family genes could be induced by various hormones and respond to various stresses. PeYUCCA6, PeYUCCA11, and PeYUCCA16 showed low or no expression in the leaves of Tainong and Golden Passion Fruit, but PeYUCCA23 had a high expression suggesting its predominant role in the plant development. The treatment of 100 μmol·L⁻¹ IAA significantly elevated the expressions of PeYUCCA7, PeYUCCA13, PeYUCCA17, PeYUCCA24, and PeYUCCA26. Conclusion The expressions of YUCCAs in P. edulis varied greatly under IAA treatment. But as a family, the genes likely played an important role in the growth, development, and resistance to adverse environment of passion fruits.
- Passiflora edulis Sims,
- IAA,
- YUCCA family,
- bioinformatics,
- quantitative analysis

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References(29)

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