Functions of <i>MeUGT25</i> in Resistance of Cassava to Bacterial Wilt Disease

ZENG Jian; CHEN Yingtong; CAI Meiqi; LI Lizhen; LIN Man; LIU Siwen; LIU Boting; HU Wei; ZENG Liwang

doi:10.19303/j.issn.1008-0384.2023.12.009

Volume 38 Issue 12

Dec. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(12): 1453-1458

ZENG J, CHEN Y T, CAI M Q, et al. Functions of MeUGT25 in Resistance of Cassava to Bacterial Wilt Disease [J]. Fujian Journal of Agricultural Sciences，2023，38（12）：1453−1458 doi: 10.19303/j.issn.1008-0384.2023.12.009

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ZENG J, CHEN Y T, CAI M Q, et al. Functions of MeUGT25 in Resistance of Cassava to Bacterial Wilt Disease [J]. Fujian Journal of Agricultural Sciences，2023，38（12）：1453−1458 doi: 10.19303/j.issn.1008-0384.2023.12.009

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Functions of MeUGT25 in Resistance of Cassava to Bacterial Wilt Disease

doi: 10.19303/j.issn.1008-0384.2023.12.009

1.
Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region/College of Biology and Agriculture, Shaoguan University, Shaoguan, Guangdong　512005, China
2.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, Hainan　571101, China
3.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, Guangdong　510642, China

Received Date: 2023-07-21
Rev Recd Date: 2023-10-11

Available Online: 2023-12-21

Publish Date: 2023-12-28

Abstract

Abstract

Objective Disease resistance to Xamthomonas axonopodis pv. Manihotis (Xam) of cassava related to MeUGT25, a UDP-glycosyltransferases (UGT) gene, was studied for breeding purposes. Method MeUGT25 was cloned from cassava leaves (SC124) by RT-PCR. Subsequently, virus-induced gene silencing (VIGS) andXam infection challenge experiment were conducted to confirm the disease resistance of the plant. Result The expression of MeUGT25 was significantly induced by the presence of Xam. In 3 transgenic plants, qRT-PCR showed reductions in MeUGT25 expression by 71%, 70%, and 69%. In 6 d after an Xam−inoculation, the bacterial counts on the leaves of MeUGT25V-2 and MeUGT25V-3 plants increased significantly, but not of MeUGT25V-1. On the other hand, apparent plaques appeared on the leaves of the MeUGT25 gene silencing plants indicating the lowered MeUGT25 expression had significantly reduced the resistance of cassava to Xam infection. Conclusion Reduction of MeUGT25 expression in cassava mitigated the ability of the leaves to resist invasion by Xam suggesting a positive regulatory role of the gene played in the disease resistance.
- Cassava,
- MeUGT,
- biotic stress,
- VIGS,
- Xamthomonas axonopodis pv. Manihotis

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References(28)

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