Physiology and Expression of <i>PIP</i> of<i> Hippophae rhamnoides</i> under Salt Stress

SHI Hui; YAN Wenwen; YIN Daihao; ZHANG Yu; WANG Wei; BAO Yuying; MA Yuhua

doi:10.19303/j.issn.1008-0384.2023.12.004

Volume 38 Issue 12

Dec. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(12): 1414-1419

SHI H, YAN W W, YIN D H, et al. Physiology and Expression of PIP of Hippophae rhamnoides under Salt Stress [J]. Fujian Journal of Agricultural Sciences，2023，38（12）：1414−1419 doi: 10.19303/j.issn.1008-0384.2023.12.004

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SHI H, YAN W W, YIN D H, et al. Physiology and Expression of PIP of Hippophae rhamnoides under Salt Stress [J]. Fujian Journal of Agricultural Sciences，2023，38（12）：1414−1419 doi: 10.19303/j.issn.1008-0384.2023.12.004

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Physiology and Expression of PIP of Hippophae rhamnoides under Salt Stress

doi: 10.19303/j.issn.1008-0384.2023.12.004

1.
College of Agriculture and Husbandry, Qinghai University, Xining, Qinghai　810016, China

Received Date: 2023-03-01
Rev Recd Date: 2023-07-22

Available Online: 2023-12-21

Publish Date: 2023-12-28

Abstract

Abstract

Objective Mechanism of aquaporins relating to the plasma membrane intrinsic proteins (PIPs) of Hippophae rhamnoides subsp. sinensis in response to salt stress was studied. Methods Bioinformatics of HrPIP was analyzed. Physiological indexes and expressions of the gene in tissues of H. rhamnoides under normal and imposed salt stresses of 200, 400, 600, 800, or 1,000 mmol ·L⁻¹ NaCl were determined. Results Located in the cell membrane, HrPIP encoded 110 amino acids. The hydrophobic protein had no signal peptide or transmembrane helix region. As salt stress intensified, the RWC decreased gradually, and the chlorophyll content declined initially followed by an incline. The permeability of the plasma membrane and the content of MDA changed slightly when the salt concentration was low but increased significantly when the concentration was high. The 1000 mmol·L⁻¹ salt stress induced declined MDA but continuously rising membrane permeability. The expressions of HrPIP in different organs varied by the increasing salt stress. In roots, it rose at lower salt concentrations then declined but rose again at high salt levels. In the leaves, the opposite trend was observed, whereas it was in an “M” pattern in the stems. Conclusion H.rhamnoides subsp. sinensis was salt resistant to a certain degree. Under the stress, by altering the HrPIP expression to increase water absorption in roots, transport in stems, and retention in leaves the plant manipulated the cellular salt concentration to achieve an improved stress tolerance.
- Hippophae rhamnoides subsp. sinensis,
- HrPIP,
- salt stress,
- expression pattern

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