Optimized Medium and Culture Conditions for Germination of <i>Oncidium</i> Pollens

LUO Yuanhua; CHEN Yan; FANG Nengyan; LIN Rongyan; ZHONG Huaiqin; HUANG Minling

doi:10.19303/j.issn.1008-0384.2023.11.005

Volume 38 Issue 11

Nov. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(11): 1293-1301

LUO Y H, CHEN Y, FANG N Y, et al. Optimized Medium and Culture Conditions for Germination of Oncidium Pollens [J]. Fujian Journal of Agricultural Sciences，2023，38（11）：1293−1301 doi: 10.19303/j.issn.1008-0384.2023.11.005

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LUO Y H, CHEN Y, FANG N Y, et al. Optimized Medium and Culture Conditions for Germination of Oncidium Pollens [J]. Fujian Journal of Agricultural Sciences，2023，38（11）：1293−1301 doi: 10.19303/j.issn.1008-0384.2023.11.005

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Optimized Medium and Culture Conditions for Germination of Oncidium Pollens

doi: 10.19303/j.issn.1008-0384.2023.11.005

Crop Research Institute/Flower Research Center/Fujian Engineering Research Center for Characteristic Floriculture, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China

Received Date: 2023-08-21
Rev Recd Date: 2023-10-13

Available Online: 2023-12-21

Publish Date: 2023-11-28

Abstract

Abstract

Objective Medium formulation and culture conditions were optimized for in vitro pollen germination of Oncidium spp. Methods On a liquid culture medium, in vitro germination rates of the pollens collected from the flowers of various Oncidium germplasms on the day of full bloom were determined. Based on the effects of sucrose, H₃BO₃, Ca(NO3)·4H2O, MgSO4·7H2O, and KNO3 on the germination rate, an orthogonal experiment was applied to optimize the medium formulation and culture conditions. Subsequently, on the optimized medium under selected conditions, in vitro pollen germination rates of 68 collected Oncidium germplasms were determined. Results The degrees of importance of the medium ingredients to the pollen germination were found to be H₃BO₃＞Ca(NO3)·4H2O＞sucrose＞MgSO4·7H2O＞KNO3. The optimized formulation consisted of 100 g·L⁻¹ of sucrose, 10 mg·L⁻¹ of H₃BO₃, 50 mg·L⁻¹ of Ca(NO3)·4H2O, 20 mg·L⁻¹ of MgSO4·7H2O, and 30 mg·L⁻¹ of KNO3. The pollen culture was conducted at 25 ℃ for 48h in darkness to deliver a generally low germination rate of 0.74% to 49.65% on 60.29% of the Oncidium germplasms. Conclusion The liquid medium and culture conditions for an in vitro pollen germination of Oncidium were optimized. The germination rates differed significantly among the species.
- Oncidium spp.,
- pollen,
- in vitro germination,
- liquid medium,
- germination rate

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References(27)

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