Identification and Functions of Caffeic Acid O-methyltransferase Genes in Maize

ZHANG Lu; ZHAO Yueqiang; FU Jiafeng; ZHANG Quan; MA Chunye; LI Yingzhuang; ZHANG Lan; WANG Gaige

doi:10.19303/j.issn.1008-0384.2023.11.001

Volume 38 Issue 11

Nov. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(11): 1259-1266

ZHANG L, ZHAO Y Q, FU J F, et al. Identification and Functions of Caffeic Acid O-methyltransferase Genes in Maize [J]. Fujian Journal of Agricultural Sciences，2023，38（11）：1259−1266 doi: 10.19303/j.issn.1008-0384.2023.11.001

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ZHANG L, ZHAO Y Q, FU J F, et al. Identification and Functions of Caffeic Acid O-methyltransferase Genes in Maize [J]. Fujian Journal of Agricultural Sciences，2023，38（11）：1259−1266 doi: 10.19303/j.issn.1008-0384.2023.11.001

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Identification and Functions of Caffeic Acid O-methyltransferase Genes in Maize

doi: 10.19303/j.issn.1008-0384.2023.11.001

Luohe Academy of Agricultural Sciences, Luohe, Henan　462000, China

Received Date: 2023-03-13
Rev Recd Date: 2023-07-30

Available Online: 2023-12-21

Publish Date: 2023-11-28

Abstract

Abstract

Objective Localization in genome and response to salt stress of the genes of caffeic acid O-methyltransferase (COMT), a key melatonin biosynthesis enzyme relating to plant growth, development, and stress resistance, in maize were studied. Method Structure, phylogenetics, and expressions of the COMT family in maize (ZmCOMT) were analyzed. Functions of the genes were verified by overexpressing ZmCOMT12 in Arabidopsis thaliana. Result Twenty-eight COMTs were identified in the maize genome and phylogenetically divided into Branch Ⅰ and Branch II. Most of them were structurally similar with a like motif composition, and some had tissue specific expressions. As revealed by RT-PCR, ZmCOMTs could be induced by salt stress, indicating its association with the stress regulation. The overexpressed ZmCOMT12 significantly elevated the melatonin content and salt tolerance of Arabidopsis. Conclusion ZmCOMT12 was hypothesized as the melatonin synthesizing gene by the sequence alignment, genetic relationship, and protein structure analyses.
- Maize,
- caffeic acid O-methyltransferase,
- melatonin,
- abiotic stress,
- member identification

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References(19)

References

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