Effects of Straw-returning on Phosphorus Morphology and Microbial Phosphorus-cycling Genes in Rice Paddy Soil

LI Ji; ZHANG Qiao; ZHANG Jiaxin; HAN Xinghua; LI Wanling; SUN Lili; GU Wenjie; LU Yusheng; XIE Kaizhi; XU Peizhi

doi:10.19303/j.issn.1008-0384.2023.10.012

Volume 38 Issue 10

Oct. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(10): 1230-1241

LI J, ZHANG Q, ZHANG J X, et al. Effects of Straw-returning on Phosphorus Morphology and Microbial Phosphorus-cycling Genes in Rice Paddy Soil [J]. Fujian Journal of Agricultural Sciences，2023，38（10）：1230−1241 doi: 10.19303/j.issn.1008-0384.2023.10.012

Citation:

LI J, ZHANG Q, ZHANG J X, et al. Effects of Straw-returning on Phosphorus Morphology and Microbial Phosphorus-cycling Genes in Rice Paddy Soil [J]. Fujian Journal of Agricultural Sciences，2023，38（10）：1230−1241 doi: 10.19303/j.issn.1008-0384.2023.10.012

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Effects of Straw-returning on Phosphorus Morphology and Microbial Phosphorus-cycling Genes in Rice Paddy Soil

doi: 10.19303/j.issn.1008-0384.2023.10.012

LI Ji^{1, 2
,},
ZHANG Qiao³,
ZHANG Jiaxin^{1, 2},
HAN Xinghua^{1, 2},
LI Wanling²,
SUN Lili²,
GU Wenjie^{2, 4},
LU Yusheng²,
XIE Kaizhi^{1, 2
,
,},
XU Peizhi^{1, 2
,
,}

1.
College of Resources and Environment, Gansu Agricultural University, Lanzhou, Gansu　730070, Chinas
2.
Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer in South China, Ministry of Agriculture/Guangdong Key Laboratory of Nutrient Resources Recycling and Cultivated Land Conservation/Guangdong Engineering Research Center of Soil Microbiology and Cultivated Land Conservation, Guangzhou, Guangdong　510640, China
3.
Guangdong Agricultural Environment and Cultivated Land Quality Protection Center, Guangzhou, Guangdong　510500, China
4.
Maoming Branch, Guangdong Provincial Laboratory of Modern Agricultural Science and Technology of Lingnan, Maoming, Guangdong　525000, China

Received Date: 2023-04-10
Accepted Date: 2023-04-10
Rev Recd Date: 2023-05-17

Available Online: 2023-10-25

Publish Date: 2023-10-28

Abstract

Abstract

Objective Effects of straw-returning on phosphorus morphology and microbial phosphorus-cycling genes in paddy soil at rice tillering and maturing stages were investigated. Method In consecutive 7 years on a rice field in southern China under a positioning experiment, spent straws were returned to the acidic soil. The implemented treatments included: (1) chemical fertilizer without straw-returning (CK), (2) chemical fertilizer + 100% straw-returning in same season (CKS), (3) CKS+ straws to replace 10% potassium fertilizer (S10), (4) CKS + straws to replace 20% potassium fertilizer (S20) or (5) CKS + straws to replace 30% potassium fertilizer (S30). At end of the treatments, Guppy soil phosphorus continuous extraction method and metagenomic technology were applied to determine the composition of phosphorus of different forms and microbial phosphorus-cycling genes. Result Straw-returning significantly increased the available NaHCO₃-Pi in soil (P＜0.05)—the S10 and S20 treatments resulted in an increase by 5.88%-8.73% over CK. NaOH-Pi was the main form of phosphorus in the acid paddy soil in southern China with a content ranging from 154.03 mg·kg⁻¹ to 202.11 mg·kg⁻¹. By turning the spent straws into the field, the abundance of phosphorus-cycling genes, especially the inorganic phosphorus dissolution gene pqqC under CKS, was significantly affected. The genes, such as phnW, phnO, pqqB, and pqqC, activated the conversion of hydrochloric acid phosphorus and residual phosphorus into available form; those like appA, phnX, and ppx, participated in the formation of stable phosphorus; and NaOH-Pi played a key role in the long-term transformation of the mineral. And the main factors that governed the abundance of the functional genes appeared to be the organic carbon and pH of the soil. Conclusion Through altering the soil physiochemical properties, returning spent straws to the ground significantly enriched the microbial phosphorus-cycling genes that promoted the mineral transformation of the acidic paddy soil in southern China.
- Straw-returning,
- phosphorus forms,
- phosphorus-cycling genes,
- metagenomics

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References(42)

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