Pathogen of Branch Blight on Blueberry Plants in Fujian

ZHANG Xiaoyan; CHEN Xihong; XIE Lixue; ZHANG Lijie; ZHENG Shan; LI Tao

doi:10.19303/j.issn.1008-0384.2023.10.010

Volume 38 Issue 10

Oct. 2023

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ZHANG X Y, CHEN X H, XIE L X, et al. Pathogen of Branch Blight on Blueberry Plants in Fujian [J]. Fujian Journal of Agricultural Sciences，2023，38（10）：1214−1219 doi: 10.19303/j.issn.1008-0384.2023.10.010

Citation:

ZHANG X Y, CHEN X H, XIE L X, et al. Pathogen of Branch Blight on Blueberry Plants in Fujian [J]. Fujian Journal of Agricultural Sciences，2023，38（10）：1214−1219 doi: 10.19303/j.issn.1008-0384.2023.10.010

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Pathogen of Branch Blight on Blueberry Plants in Fujian

doi: 10.19303/j.issn.1008-0384.2023.10.010

1.
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Technology Center of Fuzhou Customs District, Fuzhou, Fujian　350001, China

Received Date: 2023-06-30
Rev Recd Date: 2023-07-26

Available Online: 2023-10-25

Publish Date: 2023-10-28

Abstract

Abstract

Objective The pathogen that caused the blueberry branch blight in Sanming, Fujian was identified. Method Microbes were isolated and purified from the blueberry branches showing anthracnose symptoms collected from the infected area. Pathogenicity of the isolates was tested by needle injection on the plant and verified by the Koch's method. Pathogen taxonomy was determined by morphological observations and molecular technology. Result Strain KW1-4 was isolated from the diseased tissue and inoculated onto healthy blueberry branches to show on the inoculation site dry spots same as what produced by the strain from the infected plants. The white to gray, round colonies of KW1-4 had two types of conidia. The alpha conidia were elliptical or spindle in shape and 4.77-7.63 μm × 1.55-2.71 μm in size with two distinct oil globules, while the linear beta conidia sized 11.95-19.65 μm × 1.05-1.94 μm with no oil globule. The 3 fragments of the genes, ITS, TEF1-ɑ, and β-tubulin, were used to construct the phylogenetic tree that showed KW1-4 to cluster with Diaporthe australiana in a same branch, which agreed with the morphological characteristics. Conclusion It was determinedD. australiana to be the pathogen that caused the blight on blueberry bushes in Sanming. The identification paved the way for the disease control.
- Blueberry,
- branch blight,
- Diaporthe australiana,
- identification

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References(31)

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