Functions of <i>OsPtr1</i> in Rice

HU Changquan; LIU Huaqing; LI Gang; WANG Feng

doi:10.19303/j.issn.1008-0384.2023.10.001

Volume 38 Issue 10

Oct. 2023

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(10): 1133-1138

HU C Q, LIU H Q, LI G, et al. Functions of OsPtr1 in Rice [J]. Fujian Journal of Agricultural Sciences，2023，38（10）：1133−1138 doi: 10.19303/j.issn.1008-0384.2023.10.001

Citation:

HU C Q, LIU H Q, LI G, et al. Functions of OsPtr1 in Rice [J]. Fujian Journal of Agricultural Sciences，2023，38（10）：1133−1138 doi: 10.19303/j.issn.1008-0384.2023.10.001

HU C Q, LIU H Q, LI G, et al. Functions of OsPtr1 in Rice [J]. Fujian Journal of Agricultural Sciences，2023，38（10）：1133−1138 doi: 10.19303/j.issn.1008-0384.2023.10.001

Citation:

HU C Q, LIU H Q, LI G, et al. Functions of OsPtr1 in Rice [J]. Fujian Journal of Agricultural Sciences，2023，38（10）：1133−1138 doi: 10.19303/j.issn.1008-0384.2023.10.001

PDF( 1077 KB)

Functions of OsPtr1 in Rice

doi: 10.19303/j.issn.1008-0384.2023.10.001

Institute of Biotechnology, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agricultural Genetic Engineering, Fuzhou, Fujian　350003, China

Received Date: 2023-01-18
Rev Recd Date: 2023-03-17

Available Online: 2023-10-25

Publish Date: 2023-10-28

Abstract

Abstract

Objective Functions of a peptide transporter protein gene, OsPtr1, in rice were studied by means of T-DNA insertion mutation. Method The target RNA was extracted from the endosperm of Minghui 86 rice 12 d after flowering and reverse-transcribed into cDNA. By cloning the candidate gene P0421H01.23, named OsPtr1, and constructing the plant overexpression vector using the promoter Gt1 of endosperm-specific expressing, OsPtr1 was introduced into Nipponbare rice using an agrobacterium-mediated method. Nitrogen metabolism of the offspring and mutant w9101 was analyzed. A fusion expression vector of green fluorescent protein gene, GFP, and OsPtr1 was constructed and transferred to onion epidermal cells with a gene gun. In 24–48 h afterward, subcellular localization of OsPtr1 was examined under a fluorescence confocal electron microscope. Result OsPtr1 was localized at the membrane and possibly involved in the transport of nitrogen substances. Knockout on OsPtr1 could cause a reduction on nitrogen-containing substances, such as amino acids and proteins, in the rice grains. Conversely, an overexpressed OsPtr1 would increase the accumulation. Conclusion OsPtr1 participated in the transmembrane transport of nitrogen substances in rice.
- Rice,
- endosperm-specific expressing,
- peptide transporter,
- OsPtr1,
- nitrogen-metabolism

FullText(HTML)

References(14)

References

[1]	RAM H, SONI P, SALVI P, et al. Insertional mutagenesis approaches and their use in rice for functional genomics [J]. Plants, 2019, 8(9): 310. doi: 10.3390/plants8090310
[2]	ODA Y, KOBAYASHI N I, TANOI K, et al. T-DNA tagging-based gain-of-function of OsHKT1;4 reinforces Na exclusion from leaves and stems but triggers Na toxicity in roots of rice under salt stress [J]. International Journal of Molecular Sciences, 2018, 19(1): 235. doi: 10.3390/ijms19010235
[3]	WU H P, WEI F J, WU C C, et al. Large-scale phenomics analysis of a T-DNA tagged mutant population [J]. Giga Science, 2017, 6(8): 1−7.
[4]	CHEN S Y, WANG A M, LI W, et al. Establishing a gene trap system mediated by T-DNA(GUS) in rice [J]. Journal of Integrative Plant Biology, 2008, 50(6): 742−751. doi: 10.1111/j.1744-7909.2007.00611.x
[5]	张晖, 胡昌泉, 刘华清, 等. 一个水稻胚乳特异表达基因的筛选及初步分析 [J]. 福建农业学报, 2009, 24(1):6−10. doi: 10.3969/j.issn.1008-0384.2009.01.002 ZHANG H, HU C Q, LIU H Q, et al. Screening and preliminary analysis of an endosperm-specific expression gene in rice(Oryza sativa L. ) [J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 6−10.(in Chinese) doi: 10.3969/j.issn.1008-0384.2009.01.002
[6]	王关林, 方宏筠. 植物基因工程[M]. 2版. 北京: 科学出版社, 2002.
[7]	胡昌泉, 徐军望, 苏军, 等. 农杆菌介导法获得转可溶性淀粉合成酶基因籼稻 [J]. 福建农业学报, 2003, 18(2):65−68. doi: 10.3969/j.issn.1008-0384.2003.02.001 HU C Q, XU J W, SU J, et al. Induction of soluble starch synthase gene into Indica Staiva L. by Agrobacterium-mediated transformation [J]. Fujian Journal of Agricultural Sciences, 2003, 18(2): 65−68.(in Chinese) doi: 10.3969/j.issn.1008-0384.2003.02.001
[8]	YANG X H, XIA X Z, ZENG Y, et al. Genome-wide identification of the peptide transporter family in rice and analysis of the PTR expression modulation in two near-isogenic lines with different nitrogen use efficiency [J]. BMC Plant Biology, 2020, 20(1): 193. doi: 10.1186/s12870-020-02419-y
[9]	ZHAO J Z, YU N N, JU M, et al. ABC transporter OsABCG18 controls the shootward transport of cytokinins and grain yield in rice [J]. Journal of Experimental Botany, 2019, 70(21): 6277−6291. doi: 10.1093/jxb/erz382
[10]	KOMAROVA N Y, THOR K, GUBLER A, et al. AtPTR1 and AtPTR5 transport dipeptides in planta [J]. Plant Physiology, 2008, 148(2): 856−869. doi: 10.1104/pp.108.123844
[11]	DIETRICH D, HAMMES U, THOR K, et al. AtPTR1, a plasma membrane peptide transporter expressed during seed germination and in vascular tissue of Arabidopsis [J]. The Plant Journal:for Cell and Molecular Biology, 2004, 40(4): 488−499. doi: 10.1111/j.1365-313X.2004.02224.x
[12]	MARTINEZ MOLLEDO M, QUISTGAARD E M, LÖW C. Tripeptide binding in a proton-dependent oligopeptide transporter [J]. FEBS Letters, 2018, 592(19): 3239−3247. doi: 10.1002/1873-3468.13246
[13]	TSAY Y F, SCHROEDER J I, FELDMANN K A, et al. The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter [J]. Cell, 1993, 72(5): 705−713. doi: 10.1016/0092-8674(93)90399-B
[14]	FANG Z M, XIA K F, YANG X, et al. Altered expression of the PTR/NRT1 homologue OsPTR9 affects nitrogen utilization efficiency, growth and grain yield in rice [J]. Plant Biotechnology Journal, 2013, 11(4): 446−458. doi: 10.1111/pbi.12031