Identification and Inhibitory Effect on <i>Lasiodiplodia theobromae</i> of Actinomycetes in <i>Camellia sinensis</i> Rhizosphere Soil

CHEN Xiaochen; XU Jinrong; GAO Peiyan; LUO Zhengduo; ZHENG Zhisheng; WU Baochuan; ZHOU Yan; HUANG Zhaobin; CHEN Hongbin; ZHANG Qiufang

doi:10.19303/j.issn.1008-0384.2023.09.012

Volume 38 Issue 9

Sep. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(9): 1103-1111

CHEN X C, XU J R, GAO P Y, et al. Identification and Inhibitory Effect on Lasiodiplodia theobromae of Actinomycetes in Camellia sinensis Rhizosphere Soil [J]. Fujian Journal of Agricultural Sciences，2023，38（9）：1103−1111 doi: 10.19303/j.issn.1008-0384.2023.09.012

Citation:

CHEN X C, XU J R, GAO P Y, et al. Identification and Inhibitory Effect on Lasiodiplodia theobromae of Actinomycetes in Camellia sinensis Rhizosphere Soil [J]. Fujian Journal of Agricultural Sciences，2023，38（9）：1103−1111 doi: 10.19303/j.issn.1008-0384.2023.09.012

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Identification and Inhibitory Effect on Lasiodiplodia theobromae of Actinomycetes in Camellia sinensis Rhizosphere Soil

doi: 10.19303/j.issn.1008-0384.2023.09.012

1.
College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, Fujian　362000, China
2.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350007, China
3.
Research Monitoring Center, Wuyishan National Park, Nanping, Fujian　354300, China

Received Date: 2023-04-17
Rev Recd Date: 2023-06-13

Available Online: 2023-10-13

Publish Date: 2023-09-28

Abstract

Abstract

Objective Actinomycetes collected from the rhizosphere soils of old tea plants were isolated to screen for species unknown previously and their potential pathogenic inhibitory effect. Method Soil specimens were gathered from the target lots at Shuixian, Fujian to isolate actinomycetes by plate culture. Phylogenetic trees based on 16S rRNA gene for the isolates were constructed and diversity index calculated in the tea plant rhizosphere soils. Result (1) Eighty-one actinomycete strains were obtained that belonged to Streptomyces(54.32%), Arthrobacter (27.16%), Microbacterium (11.11%), Kitasatospora(4.94%), and Curtobacterium (2.47%). (2) In comparing with the reference strain, 20 of the 81 taxa showed less than 98.65% similarity. (3) Seven strains of the Streptomyces significantly inhibited the growth of Lasiodiplodia theobromae, of which, the one code-named WYS-24 reached the highest rate at 63.92%. Conclusion Abundantly diverse actinomycetes resided in the rhizosphere of old tea plants in Wuyishan region. They were a rich resource for in-depth studies and development for biological inhibitors against plant pathogens.
- Rhizosphere,
- Camellia sinensis,
- Lasiodiplodia theobromae,
- 16S rRNA gene

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References(49)

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