Effect of Curcumin on <i>HSP</i> Expression in Tilapia Hepatocytes under Hydrogen Peroxide Stress

LI Linming; ZHANG Ziping; WANG Ruoxuan; HUANG Yifan

doi:10.19303/j.issn.1008-0384.2023.09.004

Volume 38 Issue 9

Sep. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(9): 1030-1036

LI L M, ZHANG Z P, WANG R X, et al. Effect of Curcumin on HSP Expression in Tilapia Hepatocytes under Hydrogen Peroxide Stress [J]. Fujian Journal of Agricultural Sciences，2023，38（9）：1030−1036 doi: 10.19303/j.issn.1008-0384.2023.09.004

Citation:

LI L M, ZHANG Z P, WANG R X, et al. Effect of Curcumin on HSP Expression in Tilapia Hepatocytes under Hydrogen Peroxide Stress [J]. Fujian Journal of Agricultural Sciences，2023，38（9）：1030−1036 doi: 10.19303/j.issn.1008-0384.2023.09.004

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Effect of Curcumin on HSP Expression in Tilapia Hepatocytes under Hydrogen Peroxide Stress

doi: 10.19303/j.issn.1008-0384.2023.09.004

LI Linming^{1, 2
,},
ZHANG Ziping^{1, 2
,
,},
WANG Ruoxuan^{1, 2},
HUANG Yifan³

1.
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
College of Marine Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
3.
Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2023-02-13
Rev Recd Date: 2023-06-21

Available Online: 2023-10-25

Publish Date: 2023-09-28

Abstract

Abstract

Objective Expression of heat shock protein (HSP) gene under oxidative stress in the presence of curcumin was studied to understand the toxicological function of the protein and the pharmacological mechanism of curcumin in hepatocytes of Oreochromis nilotica. Methods Tilapia hepatocytes and those pretreated with curcumin for 24 h were subjected to H₂O₂ stress for 0, 1, or 2.5 h. The expressions of HSP70, HSP90α, HSP90β, HSP60, and HSP75, as well as 5 small molecule HSP genes, i.e., HSP30, HSPB1, HSPB7, HSPB8, and HSPB11, in the cells were determined by qPCR. Results The H₂O₂ treatment for 1 h significantly increased the expression of HSPB1 and decreased that of HSP30 in the tilapia hepatocytes, while the treatment that lasted for 2.5 h significantly increased the expressions of HSP90a and HSPB1. A pretreatment of curcumin for 24 h on the cells not only significantly elevated the expressions of HSP70, HSP90α, HSP30, and HSPB1 over control but also on those of HSP70, HSP90α, and HSP30 under 1 h H₂O₂ stress, as well as those on HSP90α, HSP30, and HSPB1 under 2.5 h H₂O₂ stress. However, no significant differences in other HSPs were observed. Conclusion As a spontaneous response of tilapia hepatocytes to oxidative stress, H₂O₂ affected significantly the expressions of HSP90a, HSP30, and HSPB1 in the cells. By pretreating the hepatocytes with curcumin, expressions of these genes could be uplifted boosting the cellular antioxidant capacity to better resist the stress and maintain viability.
- Tilapia,
- curcumin,
- heat shock proteins,
- oxidative stress

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References(40)

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