SSR Markers Associated with Sex-difference of <i>Populus yunnanensis</i> Dode

LI Qishao; LEI Han; SHEN Weixiang; ZHAO Wenzhi; WANG Fei; XU Jian; QU Shaohong; DONG Zhanghong; XIN Peiyao

doi:10.19303/j.issn.1008-0384.2023.08.009

Volume 38 Issue 8

Aug. 2023

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Fujian Journal of Agricultural Sciences > 2023 > 38(8): 953-959. > DOI: 10.19303/j.issn.1008-0384.2023.08.009

LI Q S, LEI H, SHEN W X, et al. SSR Markers Associated with Sex-difference of Populus yunnanensis Dode [J]. Fujian Journal of Agricultural Sciences，2023，38（8）：953−959. DOI: 10.19303/j.issn.1008-0384.2023.08.009

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SSR Markers Associated with Sex-difference of Populus yunnanensis Dode

LI Qishao^{1, 2,},
LEI Han³,
SHEN Weixiang^{1, 2},
ZHAO Wenzhi^{1, 2},
WANG Fei^{1, 2},
XU Jian²,
QU Shaohong⁴,
DONG Zhanghong^{1, 2},
XIN Peiyao^{1, 2, ,}

1.
Southwest Research Center for Landscape Architecture Engineering of State Forestry and Grassland Administration, Southwest Forestry University, Kunming,Yunnan　650224, China
2.
Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, Yunnan　650224, China
3.
Beijing Science and Technology Development Center of Ministry of Agriculture and Rural Affairs, Beijing　102600, China
4.
Kaiyuan Baituqiang National Forest Farm, Kaiyuan, Yunnan　661600, China

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Received Date: January 11, 2023
Revised Date: June 25, 2023
Available Online: August 15, 2023

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Abstract

Abstract

Objective Means utilizing molecular markers to differentiate rapidly and accurately male and female Populus yunnanensis plants at young stages was investigated.
Method Using simple sequence repeats (SSR), molecular markers were screened for those might associate with the sex of P. yunnanensis at seedling stages and before flowering.
Result Fifteen pairs of SSR primers were selected from the reported literatures. One of them, coded BPCA90, showed clear bands and stable reaction in agarose gel detection. After rescreening the primer by polyacrylamide gel electrophoresis, a specific band of approximately 680 bp appeared in the PCR amplification of male P. yunnanensis. Subsequent testing on 30 plants using these SSR primers showed significantly more males than females in a ratio of 13 to 2.
Conclusion Short of a visible morphological sign for the determination, the SSR primers screened by this study appeared to suffice the general purpose of differentiating the sex of P. yunnanensis at early growth stage.
- Populus yunnanensis Dode,
- SSR,
- primer screening,
- gender identification

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References

[1]	王德新, 张晏, 段安安, 等. 滇杨优树无性系物候期观测 [J]. 西南林学院学报, 2009, 29(6):20−23,27. WANG D X, ZHANG Y, DUAN A A, et al. Observation on phenological phase of Populus yunnanensis plus tree clones [J]. Journal of Southwest Forestry University, 2009, 29(6): 20−23,27.(in Chinese)
[2]	CHEN L H, ZHANG S, ZHAO H X, et al. Sex-related adaptive responses to interaction of drought and salinity in Populus yunnanensis [J]. Plant, Cell & Environment, 2010, 33(10): 1767−1778.
[3]	LI L, ZHANG Y B, LUO J X, et al. Sex-specific responses of Populus yunnanensis exposed to elevated CO₂ and salinity [J]. Physiologia Plantarum, 2013, 147(4): 477−488. DOI: 10.1111/j.1399-3054.2012.01676.x
[4]	LI X, YANG Y Q, SUN X D, et al. Comparative physiological and proteomic analyses of poplar (Populus yunnanensis) plantlets exposed to high temperature and drought [J]. PLoS One, 2014, 9(9): e107605. DOI: 10.1371/journal.pone.0107605
[5]	LIAO L Q, LIU J, DAI Y X, et al. Development and application of SCAR markers for sex identification in the dioecious species Ginkgo biloba L. [J]. Euphytica, 2009, 169(1): 49−55. DOI: 10.1007/s10681-009-9913-8
[6]	李仕金, 辛培尧, 郭鸿彦, 等. 大麻雄性相关RAPD和SCAR标记的研究 [J]. 广东农业科学, 2012, 39(24):151−154. DOI: 10.3969/j.issn.1004-874X.2012.24.047 LI S J, XIN P Y, GUO H Y, et al. Study of male-specific RAPD and SCAR marker in Cannabis sativa L [J]. Guangdong Agricultural Sciences, 2012, 39(24): 151−154.(in Chinese) DOI: 10.3969/j.issn.1004-874X.2012.24.047
[7]	JIANG C X, SINK K C. RAPD and SCAR markers linked to the sex expression locus M in asparagus [J]. Euphytica, 1997, 94(3): 329−333. DOI: 10.1023/A:1002958007407
[8]	MIDGLEY J J. Causes of secondary sexual differences in plants—Evidence from extreme leaf dimorphism in Leucadendron (Proteaceae) [J]. South African Journal of Botany, 2010, 76(3): 588−592. DOI: 10.1016/j.sajb.2010.05.001
[9]	ZIETKIEWICZ E, RAFALSKI A, LABUDA D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification [J]. Genomics, 1994, 20(2): 176−183. DOI: 10.1006/geno.1994.1151
[10]	SALIH KAFKAS S K, SELIM CETINER G D, PERL-TREVES R, et al. Development of sex-associated RAPD markers in wildPistaciaspecies [J]. The Journal of Horticultural Science and Biotechnology, 2001, 76(2): 242−246. DOI: 10.1080/14620316.2001.11511358
[11]	XU W J, WANG B W, CUI K M. RAPD and SCAR markers linked to sex determination in Eucommia ulmoides Oliv. [J]. Euphytica, 2004, 136(3): 233−238. DOI: 10.1023/B:EUPH.0000032741.99885.c4
[12]	GAO W J, LI R L, LI S F, et al. Identification of two markers linked to the sex locus in dioecious Asparagus officinalis plants [J]. Russian Journal of Plant Physiology, 2007, 54(6): 816−821. DOI: 10.1134/S1021443707060143
[13]	刘嘉艺, 岳俊阳, 刘永胜. 基于毛花猕猴桃基因组的性别相关SSR分子标记的开发 [J]. 合肥工业大学学报(自然科学版), 2022, 45(8):1135−1138,1146. LIU J Y, YUE J Y, LIU Y S. Development of sex-related SSR molecular markers based on the genome of Actinidia eriantha [J]. Journal of Hefei University of Technology (Natural Science), 2022, 45(8): 1135−1138,1146.(in Chinese)
[14]	黄海燕, 杜红岩, 乌云塔娜, 等. 基于杜仲转录组序列的SSR分子标记的开发 [J]. 林业科学, 2013, 49(5):176−181. DOI: 10.11707/j.1001-7488.20130523 HUANG H Y, DU H Y, WU Y, et al. Development of SSR molecular markers based on transcriptome sequencing of Eucommia ulmoides [J]. Scientia Silvae Sinicae, 2013, 49(5): 176−181.(in Chinese) DOI: 10.11707/j.1001-7488.20130523
[15]	马霞霞. 基于BSA分析的山葡萄性别位点定位研究[D]. 沈阳: 沈阳农业大学, 2018. MA X X. Mapping of sex locus based on BSA analysis in Vitis amurensis[D]. Shenyang: Shenyang Agricultural University, 2018. (in Chinese)
[16]	王洪梅, 李春明, 白卉, 等. 一种鉴定山杨性别的SSR分子标记的筛选 [J]. 东北林业大学学报, 2017, 45(10):17−19,29. DOI: 10.3969/j.issn.1000-5382.2017.10.005 WANG H M, LI C M, BAI H, et al. Screening SSR markers for sex identification in Populus davidiana dode [J]. Journal of Northeast Forestry University, 2017, 45(10): 17−19,29.(in Chinese) DOI: 10.3969/j.issn.1000-5382.2017.10.005
[17]	雷瀚, 刘成, 唐军荣, 等. 滇杨性别相关的SRAP分子标记 [J]. 中南林业科技大学学报, 2020, 40(8):139−146. LEI H, LIU C, TANG J R, et al. SRAP molecular markers related to sexuality of Populus yunnanensis Dode [J]. Journal of Central South University of Forestry & Technology, 2020, 40(8): 139−146.(in Chinese)
[18]	陈学军, 周坤华, 宗洪霞, 等. 中国灌木辣椒种质遗传多样性的SRAP和SSR分析 [J]. 西北植物学报, 2012, 32(11):2201−2205. DOI: 10.3969/j.issn.1000-4025.2012.11.009 CHEN X J, ZHOU K H, ZONG H X, et al. Genetic diversity of Capsicum frutescens in China as revealed by SRAP and SSR markers [J]. Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(11): 2201−2205.(in Chinese) DOI: 10.3969/j.issn.1000-4025.2012.11.009
[19]	NAGARAJA G M, MAHESH G, SATISH V, et al. Genetic mapping of Z chromosome and identification of W chromosome-specific markers in the silkworm, Bombyx mori [J]. Heredity, 2005, 95(2): 148−157. DOI: 10.1038/sj.hdy.6800700
[20]	SHAN T F, PANG S J. Sex-linked microsatellite marker detected in the female gametophytes of Undaria pinnatifida (Phaeophyta) [J]. Phycological Research, 2010, 58(3): 171−176. DOI: 10.1111/j.1440-1835.2010.00575.x
[21]	PAKULL B, GROPPE K, MECUCCI F, et al. Genetic mapping of linkage group XIX and identification of sex-linked SSR markers in a Populus tremula × Populus tremuloides cross [J]. Canadian Journal of Forest Research, 2011, 41(2): 245−253. DOI: 10.1139/X10-206
[22]	PAKULL B, GROPPE K, MEYER M, et al. Genetic linkage mapping in aspen (Populus tremula L. and Populus tremuloides Michx. ) [J]. Tree Genetics & Genomes, 2009, 5(3): 505−515.
[23]	JAKSE J, STAJNER N, KOZJAK P, et al. Trinucleotide microsatellite repeat is tightly linked to male sex in hop (Humulus lupulus L. ) [J]. Molecular Breeding, 2008, 21(2): 139−148. DOI: 10.1007/s11032-007-9114-x
[24]	CHEN S L, JI X S, SHAO C W, et al. Induction of mitogynogenetic diploids and identification of WW super-female using sex-specific SSR markers in half-smooth tongue sole (Cynoglossus semilaevis) [J]. Marine Biotechnology, 2012, 14(1): 120−128. DOI: 10.1007/s10126-011-9395-2
[25]	CHARLESWORTH D. Plant sex chromosome evolution [J]. Journal of Experimental Botany, 2013, 64(2): 405−420. DOI: 10.1093/jxb/ers322
[26]	BOUALEM A, TROADEC C, CAMPS C, et al. A cucurbit androecy gene reveals how unisexual flowers develop and dioecy emerges [J]. Science, 2015, 350(6261): 688−691. DOI: 10.1126/science.aac8370
[27]	RENNER S S. Pathways for making unisexual flowers and unisexual plants: Moving beyond the “two mutations linked on one chromosome” model [J]. American Journal of Botany, 2016, 103(4): 587−589. DOI: 10.3732/ajb.1600029
[28]	CHARLESWORTH D. Plant sex determination and sex chromosomes [J]. Heredity, 2002, 88(2): 94−101. DOI: 10.1038/sj.hdy.6800016
[29]	董莉娜, 苏雪, 孙坤, 等. DNA分子标记在雌雄异株植物性别鉴定中的应用 [J]. 广西植物, 2006, 26(1):63−68. DOI: 10.3969/j.issn.1000-3142.2006.01.015 DONG L N, SU X, SUN K, et al. Applications of DNA molecular markers on sex identification of dioecious plants [J]. Guihaia, 2006, 26(1): 63−68.(in Chinese) DOI: 10.3969/j.issn.1000-3142.2006.01.015
[30]	PAKULL B, KERSTEN B, LÜNEBURG J, et al. A simple PCR-based marker to determine sex in aspen [J]. Plant Biology, 2015, 17(1): 256−261. DOI: 10.1111/plb.12217
[31]	任朝兴, 黄建昌, 肖艳, 等. 番木瓜雄性性别的RAPD和SCAR标记 [J]. 果树学报, 2007, 24(1):72−75. REN C X, HUANG J C, XIAO Y, et al. RAPD and SCAR molecular markers for male trait in Caric papaya [J]. Journal of Fruit Science, 2007, 24(1): 72−75.(in Chinese)
[32]	唐美玲, 孔瑾, 许雪峰, 等. 山葡萄性别相关AFLP标记筛选及SCAR标记转化 [J]. 园艺学报, 2008, 35(2):195−200. DOI: 10.3321/j.issn:0513-353X.2008.02.007 TANG M L, KONG J, XU X F, et al. Screening of the AFLP markers associated to the sex locus of Vitis amurensis rupr. and its conversion to SCAR marker [J]. Acta Horticulturae Sinica, 2008, 35(2): 195−200.(in Chinese) DOI: 10.3321/j.issn:0513-353X.2008.02.007
[33]	董娜, 唐晓姗, 唐琳. 毛叶山桐子性别相关ISSR分子标记的筛选与分析 [J]. 四川大学学报(自然科学版), 2016, 53(2):465−470. DONG N, TANG X S, TANG L. Screening and analysis of sex-related ISSR molecular marker in Idesia polycarpa Maxim. var. vestita Diels [J]. Journal of Sichuan University (Natural Science Edition), 2016, 53(2): 465−470.(in Chinese)
[34]	邓传良, 任映雪, 张卫丽, 等. 葎草性别相关SRAP分子标记的鉴定 [J]. 植物研究, 2014, 34(6):798−802. DENG C L, REN Y X, ZHANG W L, et al. Identification of sex related SRAP molecular marker in Humulus scandens [J]. Bulletin of Botanical Research, 2014, 34(6): 798−802.(in Chinese)
[35]	齐凤慧, 孙宏冉, 詹亚光. EST-SSR标记在水曲柳雌雄鉴定中的应用 [J]. 西北植物学报, 2015, 35(3):472−479. DOI: 10.7606/j.issn.1000-4025.2015.03.0472 QI F H, SUN H R, ZHAN Y G. Application of EST-SSR markers in the sex identification of Fraxinus mandshurica rupr [J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(3): 472−479.(in Chinese) DOI: 10.7606/j.issn.1000-4025.2015.03.0472
[36]	HOU W W, FAN J F, ZHOU F M, et al. RAPD markers related to sex locus in Populus tomentosa [J]. Frontiers of Forestry in China, 2009, 4(2): 223−226. DOI: 10.1007/s11461-009-0037-z
[37]	张洪福. 对PCR引物设计问题的研究[D]. 济南: 山东大学, 2005. ZHANG H F. Study on PCR Primer Design[D]. Jinan: Shandong University, 2005. (in Chinese)
[38]	许玉兰, 康向阳, 张瑞丽, 等. 木本植物中EST-SSR的通用性及其多态性研究 [J]. 西南林业大学学报, 2012, 32(4):94−99. DOI: 10.3969/j.issn.2095-1914.2012.04.020 XU Y L, KANG X Y, ZHANG R L, et al. Study on versatility and polymorphism of EST-SSR application to woody plants [J]. Journal of Southwest Forestry University, 2012, 32(4): 94−99.(in Chinese) DOI: 10.3969/j.issn.2095-1914.2012.04.020
[39]	徐杨, 邓丽丽, 周丽, 等. 云南松EST-SSR引物在其近缘种中通用性的研究 [J]. 西南林业大学学报, 2016, 36(1):16−20. XU Y, DENG L L, ZHOU L, et al. The transferability analysis of microsatellite markers from expressed sequence tags of Pinus yunnanensis to its close related species [J]. Journal of Southwest Forestry University, 2016, 36(1): 16−20.(in Chinese)
[40]	杨彦伶, 张亚东, 张新叶. 杨树SSR标记在柳树中的通用性分析 [J]. 分子植物育种, 2008, 6(6):1134−1138. DOI: 10.3969/j.issn.1672-416X.2008.06.018 YANG Y L, ZHANG Y D, ZHANG X Y. Transferability analysis of Populus SSR markers in Salix [J]. Molecular Plant Breeding, 2008, 6(6): 1134−1138.(in Chinese) DOI: 10.3969/j.issn.1672-416X.2008.06.018
[41]	盛文涛. 芦笋性别决定基因分子标记的筛选与开发[D]. 南昌: 南昌大学, 2010 SHENG W T. Identification and development of molecular makers associated with sex determining gene in Asparagus[D]. Nanchang: Nanchang University, 2010. (in Chinese)

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