Correlation with Cell Wall Metabolizing-related Enzymes and Microstructure Observation in Texture Differences of two Kinds of High-Quality Pear Fruit

BAI Mudan; LIU Xiaoyu; YANG Sheng; HAO Guowei; ZHANG Xiaowei; WANG Yanping; FU Baochun

doi:10.19303/j.issn.1008-0384.2023.08.005

Volume 38 Issue 8

Aug. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(8): 917-923

BAI M D, LIU X Y, YANG S, et al. Correlation with Cell Wall Metabolizing-related Enzymes and Microstructure Observation in Texture Differences of two Kinds of High-Quality Pear Fruit [J]. Fujian Journal of Agricultural Sciences，2023，38（8）：917−923 doi: 10.19303/j.issn.1008-0384.2023.08.005

Citation:

BAI M D, LIU X Y, YANG S, et al. Correlation with Cell Wall Metabolizing-related Enzymes and Microstructure Observation in Texture Differences of two Kinds of High-Quality Pear Fruit [J]. Fujian Journal of Agricultural Sciences，2023，38（8）：917−923 doi: 10.19303/j.issn.1008-0384.2023.08.005

Citation:

BAI M D, LIU X Y, YANG S, et al. Correlation with Cell Wall Metabolizing-related Enzymes and Microstructure Observation in Texture Differences of two Kinds of High-Quality Pear Fruit [J]. Fujian Journal of Agricultural Sciences，2023，38（8）：917−923 doi: 10.19303/j.issn.1008-0384.2023.08.005

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Correlation with Cell Wall Metabolizing-related Enzymes and Microstructure Observation in Texture Differences of two Kinds of High-Quality Pear Fruit

doi: 10.19303/j.issn.1008-0384.2023.08.005

Pomology Institute, Shanxi Agricultural University/Shanxi Key Laboratory of Germplasm Improvement and Utilization in Pomology, Taiyuan, Shanxi　030031, China

Received Date: 2023-03-09
Rev Recd Date: 2023-04-30

Available Online: 2023-08-16

Publish Date: 2023-08-28

Abstract

Abstract

Objective Factors contributing to the distinctively different texture of the fruits of two pear cultivars were investigated. Method Cellular microstructures of the pulp of Yuluxiangli and Daguoshuijingli pears were observed under an electron microscope SU810 . Textural firmness and brittleness, as well as contents of cell wall-modifying enzymes and relative expressions of the associated genes, of the fruits during develop stages were measured to correlate with the microstructure observations. Result ①The firmness of the pears of both varieties gradually declined during the fruit development. The force required to break into the fruit decreased 60d after flowering as the fruits were ripening. In comparison, Yuluxiangli pears were significantly softer and less brittle than Daguoshuijingli. ②At 400× magnification, the cells of Yuluxiangli pear pulp appeared larger than those of Daguoshuijingli at young stage. As the fruits expanded and matured, the cells of both varieties enlarged significantly but those of Yuluxiangli became smaller than those of Daguoshuijingli. And upon cutting, the edge of fractured Yuluxiangli pears were clean and smooth, while that of Daguoshuijingli rough and irregular. ③ In the matured fruits, the pectin methylesterase (PME) activity in Yuluxiangli pears were extremely significantly lower than that in Daguoshuijingli, whereas the polygalacturonase (PG) and β-galactosidase (β-Gal) activities extremely significantly higher. ④ The relative expressions of PME, PG, and β-Gal in Yuluxiangli were extremely significantly lower than those in Daguoshuijingli during the fruit development. And that of β-Gal was significantly positively related to the textural firmness, but that of PG to the brittleness, of the fruits for both pear varieties. Conclusion The unique texture of Yuluxiangli and Daguoshuijingli pears could not only be differentiated by their pulp cell ultrastructural differences but also closely related to the cell wall-modifying enzymes, such as, PG and β-Gal, in the fruits. Hence, the genes associated with those enzymes likely played a critical role in the texture of the pears.
- Pears,
- distinct texture,
- ultrastructure of pulp cell,
- cell wall-modifying enzymes

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References(19)

References

[1]	郭黄萍, 李晓梅, 张建功. 优质中熟红梨新品种“玉露香”(暂定名) [J]. 山西果树, 2001(1):3−4. GUO H P, LI X M, ZHANG J G. A New Red Pear Variety “Yuluxiang” with High Quality and Medium Maturity (tentative Name) [J]. Shanxi Fruits, 2001(1): 3−4.(in Chinese)
[2]	GOULAO L F, SANTOS J, DE SOUSA I, et al. Patterns of enzymatic activity of cell wall-modifying enzymes during growth and ripening of apples [J]. Postharvest Biology and Technology, 2007, 43(3): 307−318. doi: 10.1016/j.postharvbio.2006.10.002
[3]	魏建梅, 马锋旺, 关军锋, 等. 京白梨果实后熟软化过程中细胞壁代谢及其调控 [J]. 中国农业科学, 2009, 42(8):2987−2996. WEI J M, MA F W, GUAN J F, et al. Cell wall metabolism and its regulation in harvested Pyrus ussuriensis maxin. cv. jingbaili fruit during ripening [J]. Scientia Agricultura Sinica, 2009, 42(8): 2987−2996.(in Chinese)
[4]	田路明, 曹玉芬, 高源, 等. 梨品种果肉石细胞团大小对果肉质地的影响 [J]. 园艺学报, 2011, 38(7):1225−1234. TIAN L M, CAO Y F, GAO Y, et al. Effect of stone cells size and flesh texture in pear cultivars [J]. Acta Horticulturae Sinica, 2011, 38(7): 1225−1234.(in Chinese)
[5]	杨盛, 白牡丹, 郝国伟, 等. “玉露香梨”果实发育中细胞壁组分和水解酶活性的变化 [J]. 中国南方果树, 2019, 48(2):110−111, 115. YANG S, BAI M D, HAO G W, et al. Changes on cell wall components and hydrolase activity of ‘yulu xiangli’ pear during fruit developing [J]. South China Fruits, 2019, 48(2): 110−111, 115.(in Chinese)
[6]	MWANIKI M W, MATHOOKO F M, MATSUZAKI M, et al. Expression characteristics of seven members of the β-galactosidase gene family in ‘La France’ pear (Pyrus communis L. ) fruit during growth and their regulation by 1-methylcyclopropene during postharvest ripening [J]. Postharvest Biology and Technology, 2005, 36(3): 253−263. doi: 10.1016/j.postharvbio.2005.02.002
[7]	赵爱玲, 薛晓芳, 王永康, 等. 质构仪检测鲜枣果实质地品质的方法研究 [J]. 果树学报, 2018, 35(5):631−641. ZHAO A L, XUE X F, WANG Y K, et al. Measuring texture quality of fresh jujube fruit using texture analyser [J]. Journal of Fruit Science, 2018, 35(5): 631−641.(in Chinese)
[8]	高滋艺. 不同苹果品种果实发育过程中果肉质地差异的分析[D]. 杨凌: 西北农林科技大学, 2016. GAO Z Y. Differences in fruit texture of differenct apple cultivars during fruit development[D]. Yangling: Northwest A & F University, 2016. (in Chinese)
[9]	HARKER F R, WHITE A, GUNSON F A, et al. Instrumental measurement of apple texture: A comparison of the single-edge notched bend test and the penetrometer [J]. Postharvest Biology and Technology, 2006, 39(2): 185−192. doi: 10.1016/j.postharvbio.2005.10.003
[10]	范献光. ‘瑞阳’、‘瑞雪’苹果及其亲本果实发育过程中质地差异的细胞学研究[D]. 杨凌: 西北农林科技大学, 2017. FAN X G. Cytologicalstudies on texture differences in fruit development of’Ruiyang’, ’Ruixue’ and their parents[D]. Yangling: Northwest A & F University, 2017. (in Chinese)
[11]	张娟, 高滋艺, 杨惠娟, 等. ‘秦冠’和‘富士’质地差异的解剖学观察及相关酶活性研究 [J]. 西北农业学报, 2015, 24(10):88−94. ZHANG J, GAO Z Y, YANG H J, et al. Study on anatomy and fruit texture related enzymes in apple (Malus × domestica) cultivars ‘Qinguan’ and ‘fuji’ [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2015, 24(10): 88−94.(in Chinese)
[12]	李节法, 田义轲, 王彩虹, 等. 梨贝壳杉烯氧化酶基因PpKO的克隆及生物信息学分析 [J]. 园艺学报, 2010, 37(10):1575−1582. LI J F, TIAN Y K, WANG C H, et al. Cloning and bioinformatics analysis of ent-kaurene oxidase gene PpKO in pear (Pyrus pyrifolia nakai) [J]. Acta Horticulturae Sinica, 2010, 37(10): 1575−1582.(in Chinese)
[13]	白牡丹, 王彩虹, 田义轲, 等. 柱型与普通型苹果茎尖组织中GA2ox基因的序列比较及表达量分析 [J]. 西北农业学报, 2012, 21(11):124−127. BAI M D, WANG C H, TIAN Y K, et al. Sequence and expression analysis of GA2ox gene from shoots apical tissue between columnar and standard apple [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2012, 21(11): 124−127.(in Chinese)
[14]	王燕霞, 王晓蔓, 关军锋. 梨果肉质地性状分析 [J]. 中国农业科学, 2014, 47(20):4056−4066. doi: 10.3864/j.issn.0578-1752.2014.20.014 WANG Y X, WANG X M, GUAN J F. Flesh texture characteristic analysis of pear [J]. Scientia Agricultura Sinica, 2014, 47(20): 4056−4066.(in Chinese) doi: 10.3864/j.issn.0578-1752.2014.20.014
[15]	白牡丹, 付宝春, 杨盛, 等. “玉露香梨”及其亲本果实生长发育过程中质地差异 [J]. 北方园艺, 2022(19):8−14. BAI M D, FU B C, YANG S, et al. Texture difference of fruit development between ‘yuluxiang pear’ and its parents [J]. Northern Horticulture, 2022(19): 8−14.(in Chinese)
[16]	王斐, 姜淑苓, 陈秋菊, 等. 脆肉梨果实成熟过程中质地性状的变化 [J]. 果树学报, 2016, 33(8):950−958. WANG F, JIANG S L, CHEN Q J, et al. Changes in fruit texture of crisp-flesh pear during fruit ripening [J]. Journal of Fruit Science, 2016, 33(8): 950−958.(in Chinese)
[17]	庄军平, 陈维信, 吴振先, 等. 苹果采后软化研究进展 [J]. 中国农学通报, 2004, 20(4):73−77. doi: 10.3969/j.issn.1000-6850.2004.04.025 ZHUANG J P, CHEN W X, WU Z X, et al. Advance in postharvest softening of apple fruit [J]. Chinese Agricultural Science Bulletin, 2004, 20(4): 73−77.(in Chinese) doi: 10.3969/j.issn.1000-6850.2004.04.025
[18]	王中凤. 细胞壁分解酶与果实软化的关系研究进展 [J]. 中国农学通报, 2009, 25(18):126−130. WANG Z F. Research advancement in relation of enzymes for cell wall metabolism with fruit softening [J]. Chinese Agricultural Science Bulletin, 2009, 25(18): 126−130.(in Chinese)
[19]	陈凯莉, 许轲, 张贤聪, 等. 果实中果胶代谢相关酶基因的研究进展 [J]. 园艺学报, 2017, 44(10):2008−2014. CHEN K L, XU K, ZHANG X C, et al. Advances in genes information involved in pectin metabolism in fruit [J]. Acta Horticulturae Sinica, 2017, 44(10): 2008−2014.(in Chinese)