Bioinformatics and Expression of Glycerol-3-phosphate Acyltransferase 13 Gene of Maize

LI Xin; KE Ke; REN Xiaochen; LIU Haoyu; ZHANG Binglin; LIU Weijuan; ZOU Huawen

doi:10.19303/j.issn.1008-0384.2023.08.003

Volume 38 Issue 8

Aug. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(8): 901-909

LI X, KE K, REN X C, et al. Bioinformatics and Expression of Glycerol-3-phosphate Acyltransferase 13 Gene of Maize [J]. Fujian Journal of Agricultural Sciences，2023，38（8）：901−909 doi: 10.19303/j.issn.1008-0384.2023.08.003

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LI X, KE K, REN X C, et al. Bioinformatics and Expression of Glycerol-3-phosphate Acyltransferase 13 Gene of Maize [J]. Fujian Journal of Agricultural Sciences，2023，38（8）：901−909 doi: 10.19303/j.issn.1008-0384.2023.08.003

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Bioinformatics and Expression of Glycerol-3-phosphate Acyltransferase 13 Gene of Maize

doi: 10.19303/j.issn.1008-0384.2023.08.003

LI Xin^1
,,
KE Ke¹,
REN Xiaochen¹,
LIU Haoyu¹,
ZHANG Binglin^{1, 2},
LIU Weijuan^{1, 2
,
,},
ZOU Huawen^{1, 2
,
,}

1.
College of Agriculture, Yangtze University, Jingzhou, Hubei　434025, China
2.
Ministry of Agriculture and Rural Affaris Key Laboratory of Sustainable Crop Production in Middle Reaches of Yangtze River (Co-construction by Ministry and Province), Jingzhou, Hubei　434025, China

Received Date: 2022-12-02
Rev Recd Date: 2023-04-14

Available Online: 2023-09-19

Publish Date: 2023-08-28

Abstract

Abstract

Objective Bioinformatics and expressions under stresses of glycerol-3-phosphate acyltransferase (GPAT) gene family member, ZmGPAT13, in maize were studied. Method Sequences of GPAT family members in Arabidopsis thaliana and Zea mays L. were obtained from the TAIR and MaizeGDB databases. Physicochemical properties, protein structure, subcellular localization, conserved motifs, phylogenetic evolution, phosphorylation sites, and cis-acting elements of promoter sequence of the GPATs were analyzed by bioinformatic methods. Expressions of ZmGPAT13under the stresses of mannitol, NaCl, abscisic acid (ABA), high temperature at 42 ℃, and low temperature at 4 ℃ were examined by qRT-PCR. Result A transmembrane protein, ZmGPAT13 localized in mitochondria, shared the same 15 conserved motifs with other GPAT family members, and had the highest homology with AtGPAT1 in arabidopsis. It contained 62 phosphorylation sites and might interact with multiple GPATs of maize. The promoters of ZmGPAT13consisted of numerous cis-acting elements associated with the responses to low temperature and ABA stresses. The gene expressed most highly in the coleoptile, followed by the roots and the lowest in the leaves. And, relatively, the expressions were significantly elevated under the stress of mannitol, NaCl, ABA or high or low temperature. Conclusion A high evolutionary conservation seemed existed in ZmGPAT13. It was postulated that, at transcription level, ZmGPAT13 might be involved in the responses of maize plants to abiotic stresses such as drought, salt, and extreme temperatures.
- Zea mays L.,
- glycerol-3-phosphate acyltransferase,
- ZmGPAT13,
- bioinformatics,
- abiotic stresses

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References(18)

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