Screening, Identification, and Functional Study on Drought-tolerant Endophytes in Sugarcane

LUO Yanju; XIE Linyan; WU Hao; LIU Lufeng; HE Lilian; LI Fusheng

doi:10.19303/j.issn.1008-0384.2023.07.014

Volume 38 Issue 7

Jul. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(7): 873-881

LUO Y J, XIE L Y, WU H, et al. Screening, Identification, and Functional Study on Drought-tolerant Endophytes in Sugarcane [J]. Fujian Journal of Agricultural Sciences，2023，38（7）：873−881 doi: 10.19303/j.issn.1008-0384.2023.07.014

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LUO Y J, XIE L Y, WU H, et al. Screening, Identification, and Functional Study on Drought-tolerant Endophytes in Sugarcane [J]. Fujian Journal of Agricultural Sciences，2023，38（7）：873−881 doi: 10.19303/j.issn.1008-0384.2023.07.014

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Screening, Identification, and Functional Study on Drought-tolerant Endophytes in Sugarcane

doi: 10.19303/j.issn.1008-0384.2023.07.014

College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan　650201, China

Received Date: 2022-11-03
Rev Recd Date: 2023-04-11

Available Online: 2023-07-16

Publish Date: 2023-07-28

Abstract

Abstract

Objective Endophytes in sugarcane were screened for drought resistance to serve as microbial and germplasm resources. Methods Drought-tolerant microbes were isolated from a collection of 329 sugarcane endophytes at the Sugarcane Research Institute, Yunnan Agricultural University. In a simulated experiment, target strains were screened under the artificial draught condition created by using 0-45% PEG6000. Potential candidates were identified based on the morphological, physiological, and biochemical characteristics as well as 16sDNA sequences of the microbes. Optimal culture conditions for the growth, nitrogen fixation ability, and IAA secretion of the identified isolates were determined. Results Two strains that exhibited significant drought-tolerance were identified. K5 was a Kocuria sp. with an accession number of OP737815, and ZM a Bacillus sp. with an accession number of OP740361. Withstood the stress exerted by 40% PEG6000 or 100 g·L⁻¹ sodium chloride, they could grow stably at 50 ℃ in wide ranges of pHs and temperatures but optimally under an initial pH at 5.0 for K5 and 6.0 for ZM. ZM was stronger than K5 in fixing nitrogen. Within a limited timeframe, the IAA secretion of the two strains increased with prolonged culture. Conclusion Both K5 and ZM displayed significant drought-resistance with the abilities of nitrogen fixation and IAA secretion. They could be potential candidates as a microbial agent, and their carrier sugarcane a valuable germplasm.
- Sugarcane,
- endophyte,
- drought tolerance,
- screening,
- identification

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References(29)

References

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