Cloning and Expression under Drought of <i>NHX6</i> in <i>Hippophae rhamnoides</i>

LUO Chengyue; MA Yuhua; WANG Chenzhao; ZHANG Dan; DONG Jiawei; YE Guisheng

doi:10.19303/j.issn.1008-0384.2023.07.006

Volume 38 Issue 7

Jul. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(7): 809-816

LUO C Y, MA Y H, WANG C Z, et al. Cloning and Expression under Drought of NHX6 in Hippophae rhamnoides [J]. Fujian Journal of Agricultural Sciences，2023，38（7）：809−816 doi: 10.19303/j.issn.1008-0384.2023.07.006

Citation:

LUO C Y, MA Y H, WANG C Z, et al. Cloning and Expression under Drought of NHX6 in Hippophae rhamnoides [J]. Fujian Journal of Agricultural Sciences，2023，38（7）：809−816 doi: 10.19303/j.issn.1008-0384.2023.07.006

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Cloning and Expression under Drought of NHX6 in Hippophae rhamnoides

doi: 10.19303/j.issn.1008-0384.2023.07.006

College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai　810016, China

Received Date: 2022-10-23
Rev Recd Date: 2023-04-07

Available Online: 2023-08-16

Publish Date: 2023-07-28

Abstract

Abstract

Objective NHX6 of Hippophae rhamnoides was cloned and expression under drought determined to study the stress resistance mechanism. Method HrNHX6 was cloned, amplified, and sequenced for analysis by biological software. Expression of the gene under drought stress was determined. Result Located on the tonoplast, HrNHX6 had no signal peptide and contained 13 transmembrane helical regions with numerous types of modification sites, such as N-glycosylation, protein kinase phosphorylation lation, myristoylation, amidation, and sugar transporter signature. The rusults of qRT-PCR analysis showed that the expression level of HrNHX6 gene was significantly different between different tissues under draught stress, and the expression level of HrNHX6 gene was significantly different between different drought stress treatment. Conclusion The drought resistance of H. rhamnoides was closely related to HrNHX6 as shown by the structure and expression under drought stress of the gene. The important role indicated a significant venue for improving the draught resistance of the medicinal plant.
- Hippophae rhamnoides,
- HrNHX6,
- sequence analysis,
- protein structure,
- gene expression

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References(26)

References

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