Critical Sterility-inducing Temperature of Rice Affected by <i>tms5 </i>Mutation Site

WU Mingji; LIU Huaqing; CHEN Jianmin; FU Yanping; YANG Shaohua; SONG Yana; LIN Yan; WANG Feng

doi:10.19303/j.issn.1008-0384.2023.07.001

Volume 38 Issue 7

Jul. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(7): 761-771

WU M J, LIU H Q, CHEN J M, et al. Critical Sterility-inducing Temperature of Rice Affected by tms5 Mutation Site [J]. Fujian Journal of Agricultural Sciences，2023，38（7）：761−771 doi: 10.19303/j.issn.1008-0384.2023.07.001

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WU M J, LIU H Q, CHEN J M, et al. Critical Sterility-inducing Temperature of Rice Affected by tms5 Mutation Site [J]. Fujian Journal of Agricultural Sciences，2023，38（7）：761−771 doi: 10.19303/j.issn.1008-0384.2023.07.001

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Critical Sterility-inducing Temperature of Rice Affected by tms5 Mutation Site

doi: 10.19303/j.issn.1008-0384.2023.07.001

Institute of Biotechnology, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agricultural Genetic Engineering, Fuzhou, Fujian 350003, China

Received Date: 2023-05-12
Rev Recd Date: 2023-05-22

Available Online: 2023-08-16

Publish Date: 2023-07-28

Abstract

Abstract

Objective Effects of tms5 mutation site and related genetic regulatory factors in rice on the critical sterility-inducing temperature (CSIT) of the plant were investigated. Method Eleven CRISPR/Cas9 sequentially numbered T501 through T511 were designed to target 6 exons of tms5 to create mutants of Nipponbare (NIP, Oryza sativa ssp. Geng) and Minghui 86 (MH86, O. sativa ssp. Xian). Pollen male fertility and seed setting rate of the mutants were monitored under long day and high temperature field conditions in early August in Fuzhou (NHT) or at 22, 24, and 28 ℃ in phytotrons to identify the CSIT. Result The CSIT of the NIP mutants were higher than 28 ℃, while that of the MH86 mutants between 22 ℃ and 28°C. The tms5-1 T501 mutant had a significantly higher CSIT than the genetically identical tms5-2 counterparts T502, but the other 9 tm5 and the tm5-2 mutants did not differ on it. Expressions of the 3 Ub_L40 in young panicles were lower in the tms5-1 than the tm5-2 mutants of either rice varieties. Conclusion It appeared that the CSIT of tms5 mutants was affected by the genetic factors as well as the mutation site as the tms5-1 mutant T501 was shown significantly differed from the others.
- Rice,
- CRISPR/Cas9,
- TMS5,
- thermo-sensitive genic male sterility,
- critical sterility-inducing temperature

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References

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