Genome-wide Identification and Expressions under Stresses of <i>RLCK VI</i>  Family in <i>Gossypium barbadense</i>

ZHAO Zengqiang; ZHU Jincheng; LI Jieyu; LI Zhibo; LI Youzhong

doi:10.19303/j.issn.1008-0384.2023.06.007

Volume 38 Issue 6

Jun. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(6): 686-697

ZHAO Z Q, ZHU J C, LI J Y, et al. Genome-wide Identification and Expressions under Stresses of RLCK VI Family in Gossypium barbadense [J]. Fujian Journal of Agricultural Sciences，2023，38（6）：686−697 doi: 10.19303/j.issn.1008-0384.2023.06.007

Citation:

ZHAO Z Q, ZHU J C, LI J Y, et al. Genome-wide Identification and Expressions under Stresses of RLCK VI Family in Gossypium barbadense [J]. Fujian Journal of Agricultural Sciences，2023，38（6）：686−697 doi: 10.19303/j.issn.1008-0384.2023.06.007

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Genome-wide Identification and Expressions under Stresses of RLCK VI Family in Gossypium barbadense

doi: 10.19303/j.issn.1008-0384.2023.06.007

1.
Cotton Research Institute, Xinjiang Academy of Agricultural Reclamation Sciences/Xinjiang Production & Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Shihezi, Xinjiang　832000, China
2.
College of Agriculture, Shihezi University, Shihezi, Xinjiang　832000, China

Received Date: 2022-04-25
Rev Recd Date: 2022-11-27

Available Online: 2023-06-02

Publish Date: 2023-06-28

Abstract

Abstract

Objective Genomes and expressions under stresses of the RLCK VI family genes in Gossypium barbadense were determined to study the regulatory mechanisms of the growth, development, and stress resistance of cotton plants. Method Based on the latest released data on G. barbadense genome, bioinformatics of GbRLCK VI was analyzed to understand the associated physiochemical properties, sequence characteristics, gene replication, phylogenetic evolution, and expression. Result Thirty-nine RLCK VI were identified in G. barbadense which were clustered into two categories of 22 in Group A and 17 in Group B. Both groups contained a kinase domain distributed in 16 chromosomes with most of them located in plasma membrane. The gene family had undergone chromosome fragment duplication events during evolution. Since all Ka/Ks of the gene pairs were less than 1, strict purification and selection might have taken place in the process. The expressions of GbRLCK VI as shown by the transcriptome analysis varied in 10 different tissues with 11 predominantly expressed in the floral organs, while 9 in the roots, stems, and leaves. Under different stresses, 8 genes were significantly expressed by the imposed drought, salt, and verticillium wilt, and 4 only by verticillium wilt. On 4 selected genes, i.e., GB_ A12G0061, GB_ A11G2234, GB_ D01G2010, and GB_ D03G0730, qRT-PCR showed their expressions under drought, salt, or verticillium wilt stress to agree with what the transcriptome data did. Their involvement in the stress response of the cotton plant was confirmed. Conclusion The genome, structure, and phylogenetic characteristics of GbRLCK VI family in G. barbadense were determined. Their roles in the growth, development, and stress responses of the cotton plant were clarified.
- Gossypium barbadense,
- receptor-like cytoplasmic kinase,
- gene family identification,
- bioinformatics,
- expression analysis

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References(29)

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