Expression and Distribution of Melatonin Receptor Mel 1a in Tissues of Wanxi White Geese

LI Rui; ZHENG Menghao; WANG Jiaqi; LIU Yanyan; CHEN Zhihao; PANG Xunsheng; LIU Wenju; WANG Shujuan

doi:10.19303/j.issn.1008-0384.2023.06.002

Volume 38 Issue 6

Jun. 2023

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(6): 646-651

LI R, ZHENG M H, WNAG J Q, et al. Expression and Distribution of Melatonin Receptor Mel 1a in Tissues of Wanxi White Geese [J]. Fujian Journal of Agricultural Sciences，2023，38（6）：646−651 doi: 10.19303/j.issn.1008-0384.2023.06.002

Citation:

LI R, ZHENG M H, WNAG J Q, et al. Expression and Distribution of Melatonin Receptor Mel 1a in Tissues of Wanxi White Geese [J]. Fujian Journal of Agricultural Sciences，2023，38（6）：646−651 doi: 10.19303/j.issn.1008-0384.2023.06.002

Citation:

PDF( 1037 KB)

Expression and Distribution of Melatonin Receptor Mel 1a in Tissues of Wanxi White Geese

doi: 10.19303/j.issn.1008-0384.2023.06.002

1.
College of Animal Science, Anhui Science and Technology University, Fengyang, Anhui　233100, China
2.
College of Life and Health Sciences, Anhui Science and Technology University, Fengyang, Anhui 233100. China

Received Date: 2023-01-14
Rev Recd Date: 2023-04-20

Available Online: 2023-07-06

Publish Date: 2023-06-28

Abstract

Abstract

Objective Expression and distribution of Mel 1a in various organs of Wanxi White Geese were studied to reveal the regulatory mechanism of melatonin in the animal. Method Mel 1a mRNA and proteins from various tissues and organs of Wanxi White Goose were extracted for real-time PCR and western blot to determine the distribution and differential expressions. Result Mel 1a protein was widely distributed in the brain, heart, kidney, liver, lung, spleen, pancreas, and muscle cells of the geese. The IOD analysis of immunohistochemically positive signals using Image-pro plus 6 showed that the spleen had the strongest signal, followed by the pancreas. The signal from the spleen was significantly stronger than the brain, and the signal from the lungs was significantly stronger than the heart and muscles.The expression levels of Mel 1 a mRNA and protein in various organs ranked in the order of ovary, pancreas, spleen, kidney, dry, heart, brain, lungs, and muscles. And they increased gradually along with the development of follicles and decreased after the follicle diameter exceeded 3 cm. Conclusion Melatonin receptors exist in all tissues of western Anhui white geese, with the highest expression in the ovary. Melatonin can mediate the regulation of various biological functions through the receptor, especially the regulation of follicular development in western Anhui white geese.
- Melatonin receptor,
- Mel 1a,
- Wanxi white goose,
- expression,
- distribution

FullText(HTML)

References(29)

References

[1]	REITER R J, SHARMA R. Central and peripheral actions of melatonin on reproduction in seasonal and continuous breeding mammals [J]. General and Comparative Endocrinology, 2021, 300: 113620. doi: 10.1016/j.ygcen.2020.113620
[2]	NILES L P, WANG J X, SHEN L, et al. Melatonin receptor mRNA expression in human granulosa cells [J]. Molecular and Cellular Endocrinology, 1999, 156(1/2): 107−110.
[3]	AN P, WAN S T, LUO Y T, et al. Micronutrient supplementation to reduce cardiovascular risk [J]. Journal of the American College of Cardiology, 2022, 80(24): 2269−2285. doi: 10.1016/j.jacc.2022.09.048
[4]	WANG S J, LIU W J, WU C J, et al. Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2) [J]. Theriogenology, 2012, 78(7): 1517−1526. doi: 10.1016/j.theriogenology.2012.06.019
[5]	LEE C J, DO B R, LEE Y H, et al. Ovarian expression of melatonin Mel(1a) receptor mRNA during mouse development [J]. Molecular Reproduction and Development, 2001, 59(2): 126−132. doi: 10.1002/mrd.1015
[6]	LU J N, LUO Y J, MEI S H, et al. The effect of melatonin modulation of non-coding RNAs on central nervous system disorders: An updated review [J]. Current Neuropharmacology, 2021, 19(1): 3−23.
[7]	AYRE E A, WANG Z P, BROWN G M, et al. Localization and characterization of[¹²⁵I]iodomelatonin binding sites in duck gonads [J]. Journal of Pineal Research, 1994, 17(1): 39−47. doi: 10.1111/j.1600-079X.1994.tb00112.x
[8]	POON A M, CHOW P H, MAK A S, et al. Autoradiographic localization of 2[125I]iodomelatonin binding sites in the gastrointestinal tract of mammals including humans and birds [J]. Journal of Pineal Research, 1997, 23(1): 5−14. doi: 10.1111/j.1600-079X.1997.tb00328.x
[9]	POZO D, DELGADO M, FERNANDEZ-SANTOS J M, et al. Expression of the Mel1a-melatonin receptor mRNA in T and B subsets of lymphocytes from rat thymus and spleen [J]. FASEB Journal, 1997, 11(6): 466−473. doi: 10.1096/fasebj.11.6.9194527
[10]	SONG Y, PANG C S, AYRE E A, et al. Melatonin receptors in the chicken kidney are up-regulated by pinealectomy and linked to adenylate cyclase [J]. European Journal of Endocrinology, 1996, 135(1): 128−133. doi: 10.1530/eje.0.1350128
[11]	王淑娟, 刘文举, 闻爱友, 等. 鹅褪黑素受体Mel1c基因的组织表达特征 [J]. 浙江农业学报, 2018, 30(9):1454−1459. doi: 10.3969/j.issn.1004-1524.2018.09.02 WANG S J, LIU W J, WEN A Y, et al. Expression of Mel1c mRNA in various tissues of goose [J]. Acta Agriculturae Zhejiangensis, 2018, 30(9): 1454−1459.(in Chinese) doi: 10.3969/j.issn.1004-1524.2018.09.02
[12]	王淑娟, 刘文举, 刘晓丽, 等. 褪黑素受体Mel1a在鸭不同组织中的表达研究 [J]. 浙江农业学报, 2016, 28(6):928−934. WANG S J, LIU W J, LIU X L, et al. Study on expression of melatonin receptor Mel1a in several duck tissues [J]. Acta Agriculturae Zhejiangensis, 2016, 28(6): 928−934.(in Chinese)
[13]	刘文举, 王淑娟, 刘晓丽, 等. 褪黑素受体 Mel 1 b 基因mRNA和蛋白在鸭不同组织中的表达与分布 [J]. 浙江农业学报, 2018, 30(5):711−716. LIU W J, WANG S J, LIU X L, et al. Expression and distribution of Mel 1b mRNA and protein in various tissues of duck [J]. Acta Agriculturae Zhejiangensis, 2018, 30(5): 711−716.(in Chinese)
[14]	SUNDARESAN N R, MARCUS LEO M D, SUBRAMANI J, et al. Expression analysis of melatonin receptor subtypes in the ovary of domestic chicken [J]. Veterinary Research Communications, 2009, 33(1): 49−56. doi: 10.1007/s11259-008-9071-9
[15]	HE H, JIANG D M, KANG B, et al. Gene expression profiling of melatonin receptor subtypes in the ovarian hierarchical follicles of the Sichuan white goose [J]. Animal Reproduction Science, 2014, 145(1/2): 62−68.
[16]	HARDELAND R, CARDINALI D P, SRINIVASAN V, et al. Melatonin—a pleiotropic, orchestrating regulator molecule [J]. Progress in Neurobiology, 2011, 93(3): 350−384. doi: 10.1016/j.pneurobio.2010.12.004
[17]	SALLINEN P, SAARELA S, ILVES M, et al. The expression of MT₁ and MT₂ melatonin receptor mRNA in several rat tissues [J]. Life Sciences, 2005, 76(10): 1123−1134. doi: 10.1016/j.lfs.2004.08.016
[18]	NATESAN A K, CASSONE V M. Melatonin receptor mRNA localization and rhythmicity in the retina of the domestic chick, Gallus domesticus [J]. Visual Neuroscience, 2002, 19(3): 265−274. doi: 10.1017/S0952523802192042
[19]	EBISAWA T, KARNE S, LERNER M R, et al. Expression cloning of a high-affinity melatonin receptor from Xenopus dermal melanophores [J]. Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(13): 6133−6137. doi: 10.1073/pnas.91.13.6133
[20]	WIECHMANN A F, SMITH A R. Melatonin receptor RNA is expressed in photoreceptors and displays a diurnal rhythm in Xenopus retina [J]. Molecular Brain Research, 2001, 91(1/2): 104−111.
[21]	SAUZET S, BESSEAU L, HERRERA PEREZ P, et al. Cloning and retinal expression of melatonin receptors in the European Sea bass, Dicentrarchus labrax [J]. General and Comparative Endocrinology, 2008, 157(2): 186−195. doi: 10.1016/j.ygcen.2008.04.008
[22]	IKEGAMI T, MOTOHASHI E, DOI H, et al. Synchronized diurnal and circadian expressions of four subtypes of melatonin receptor genes in the diencephalon of a puffer fish with lunar-related spawning cycles [J]. Neuroscience Letters, 2009, 462(1): 58−63. doi: 10.1016/j.neulet.2009.06.076
[23]	CONFENTE F, RENDÓN M C, BESSEAU L, et al. Melatonin receptors in a pleuronectiform species, Solea senegalensis: Cloning, tissue expression, day-night and seasonal variations [J]. General and Comparative Endocrinology, 2010, 167(2): 202−214. doi: 10.1016/j.ygcen.2010.03.006
[24]	FALCÓN J, BESSEAU L, SAUZET S, et al. Mélatonine et régulations neuroendocrines chez le Poisson [J]. Journal De La Société De Biologie, 2007, 201(1): 21−29.
[25]	KULCZYKOWSKA E, KALAMARZ H, WARNE J M, et al. Day-night specific binding of 2-[125I]iodomelatonin and melatonin content in gill, small intestine and kidney of three fish species [J]. Journal of Comparative Physiology B, Biochemical, Systemic, and Environmental Physiology, 2006, 176(4): 277−285. doi: 10.1007/s00360-005-0049-4
[26]	ZHAO X M, WANG N, HAO H S, et al. Melatonin improves the fertilization capacity and developmental ability of bovine oocytes by regulating cytoplasmic maturation events [J]. Journal of Pineal Research, 2018, 64(1): 10.
[27]	AN Q L, PENG W, CHENG Y Y, et al. Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos [J]. Journal of Cellular Physiology, 2019, 234(10): 17370−17381. doi: 10.1002/jcp.28357
[28]	MASANA M I, SOARES J M Jr, DUBOCOVICH M L. 17Beta-estradiol modulates hMT1 melatonin receptor function [J]. Neuroendocrinology, 2005, 81(2): 87−95. doi: 10.1159/000084897
[29]	ZHANG W L, ZHANG Z L, PENG J, et al. Effects of melatonin on the production of GnRH and LH in luteal cells of pregnant sows [J]. Journal of Molecular Endocrinology, 2022, 68(2): 111−123. doi: 10.1530/JME-21-0155