Identification and Growth-promoting Effects of Endophytic <i>Pseudomonas</i> sp. from <i>Hippophae thibetana</i> Root Nodules

MA Fulin; RENZENG Zhuoma; WANG Changling; DENG Dekun; YE Guisheng; MA Yuhua

doi:10.19303/j.issn.1008-0384.2023.05.014

Volume 38 Issue 5

May 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(5): 624-631

MA F L, RENZENG Z M, WANG C L, et al. Identification and Growth-promoting Effects of Endophytic Pseudomonas sp. from Hippophae thibetana Root Nodules [J]. Fujian Journal of Agricultural Sciences，2023，38（5）：624−631 doi: 10.19303/j.issn.1008-0384.2023.05.014

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MA F L, RENZENG Z M, WANG C L, et al. Identification and Growth-promoting Effects of Endophytic Pseudomonas sp. from Hippophae thibetana Root Nodules [J]. Fujian Journal of Agricultural Sciences，2023，38（5）：624−631 doi: 10.19303/j.issn.1008-0384.2023.05.014

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Identification and Growth-promoting Effects of Endophytic Pseudomonas sp. from Hippophae thibetana Root Nodules

doi: 10.19303/j.issn.1008-0384.2023.05.014

Department of Agriculture and Forestry, College of Agriculture and Animal Husbandry, Qinghai University, Xining　810016, China

Received Date: 2022-10-09
Rev Recd Date: 2023-04-30

Available Online: 2023-05-09

Publish Date: 2023-05-28

Abstract

Abstract

Objective EndogenousPseudomonas sp. in the root nodules of Hippophae thibetana that displayed a biological activity were isolated for toxicological identification and growth-promoting effect determination for the development of a biological agent for crop fertilization. Methods Strains of Pseudomonas sp. were isolated from the root nodules of H. thibetana by pure culture method. They were identified by morphology, physiological biochemistry, and 16S rDNA sequence, as well as examined for the growth-promoting effect on the host plant by artificial inoculation for evidence of phosphorus-dissolving and IAA-, iron- and cellulase-producing capacities. Results Out of the isolated endophytic strains, 4 showed greater than 99% 16S rDNA alignment with the reference Pseudomonas. Among them, QY-X10 and QY-X22 produced 400 mg·L⁻¹ of solubilized phosphorus; QY-X4 yielded the highest IAA at 1.9 mg·L⁻¹; all 4 strains contained iron-generating carriers; except QY-X10, all could degrade cellulose; QY-X6 promoted seed germination; and QY-X6 and QY-X10 helped the host plant to grow significantly more leaves and longer seedlings, while QY-X10 and QY-X22 did significantly to make the maximum-length leaves longer than did control. Conclusion There were 4 Pseudomonas sp. identified in this study that exhibited significant abilities to dissolve inorganic phosphorus and produce IAA and iron. Three of them could also degrade cellulose. By inoculating these strains on the host plants, the seed germination and seedling growth were effectively promoted. It appeared that they could be applied for crop fertilization.
- Pseudomonas sp.,
- Hippophae thibetana,
- strain identification,
- growth-promoting effect

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References(26)

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