Cloning and Bioinformatics of <i>CCoAOMT</i> Relating to Resistance of Soybean to Cyst Nematodes

GUO Ziwen; SI Xiuyang; JIAO Liping; LIU Dawei

doi:10.19303/j.issn.1008-0384.2023.05.013

Volume 38 Issue 5

May 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(5): 616-623

GUO Z W, SI X Y, JIAO L P, et al. Cloning and Bioinformatics of CCoAOMT Relating to Resistance of Soybean to Cyst Nematodes [J]. Fujian Journal of Agricultural Sciences，2023，38（5）：616−623 doi: 10.19303/j.issn.1008-0384.2023.05.013

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GUO Z W, SI X Y, JIAO L P, et al. Cloning and Bioinformatics of CCoAOMT Relating to Resistance of Soybean to Cyst Nematodes [J]. Fujian Journal of Agricultural Sciences，2023，38（5）：616−623 doi: 10.19303/j.issn.1008-0384.2023.05.013

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Cloning and Bioinformatics of CCoAOMT Relating to Resistance of Soybean to Cyst Nematodes

doi: 10.19303/j.issn.1008-0384.2023.05.013

1.
College of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang　150030, China
2.
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang　310058, China

Received Date: 2022-11-10
Rev Recd Date: 2023-03-13

Available Online: 2023-05-24

Publish Date: 2023-05-28

Abstract

Abstract

Objective Full-length cDNA of Caffeoyl-CoA-O-methyltransferase gene (CCoAOMT) in the lignin metabolism pathway of soybean was cloned, and bioinformatics analyzed to study the resistance mechanism to cyst nematode (Heterodera glycines Ichinohe) of the plant. Method CCoAOMT sequence from roots of a cyst nematode-resistant Huipizhiheidou soybean plant was cloned by RT-PCR. Results The full length cDNA of GmCCoAOMT was cloned and then submitted to GenBank with the accession number of MW480860. The length of GmCCoAOMT was 848 bp, containing an ORF of 741bp and encoding 246 amino acids with a predicted molecular weight of 27.6 kDa and a theoretical isoelectric point of 5.67. It was located in the cytoplasm with a conserved AdoMet_MTases domain and belonged to that superfamily. The multiple alignment on amino acid sequence and phylogenetic tree indicated a high similarity between the gene and those of other leguminous plants. Conclusion The cDNA of GmCCoAOMT was successfully cloned and shown with a close evolutionary relationship with Vigna unguiculata (Linn.) Walp., Cajanus cajan (Linn.) Millsp., Phaseolus vulgaris Linn. and Vigna angularis (Willd.) Ohwi et Ohashi but far from Lupinus albus and Cicer arietinum Linn.
- Soybean,
- Heterodera glycines Ichinohe,
- Caffeoyl-CoA-O-methyltransferase,
- gene cloning,
- bioinformatics analysis

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References(35)

References

[1]	NOVAES E, KIRST M, CHIANG V, et al. Lignin and biomass: A negative correlation for wood formation and lignin content in trees [J]. Plant Physiology, 2010, 154(2): 555−561. doi: 10.1104/pp.110.161281
[2]	SIMMONS B A, LOQUÉ D, RALPH J. Advances in modifying lignin for enhanced biofuel production [J]. Current Opinion in Plant Biology, 2010, 13(3): 312−319. doi: 10.1016/j.pbi.2010.03.001
[3]	李雪平, 高志民, 彭镇华, 等. 绿竹咖啡酰辅酶A-O-甲基转移酶基因的克隆与分析 [J]. 分子植物育种, 2008, 6(3):587−592. doi: 10.3969/j.issn.1672-416X.2008.03.030 LI X P, GAO Z M, PENG Z H, et al. Cloning and characterization of CCoAOMT gene from Bambusa oldhamii [J]. Molecular Plant Breeding, 2008, 6(3): 587−592.(in Chinese) doi: 10.3969/j.issn.1672-416X.2008.03.030
[4]	VAN ACKER R, VANHOLME R, STORME V, et al. Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana [J]. Biotechnology for Biofuels, 2013, 6(1): 46. doi: 10.1186/1754-6834-6-46
[5]	方东鹏, 靳立梅, 董利东, 等. 野生大豆接种大豆疫霉菌后木质素含量的变化 [J]. 大豆科学, 2015, 34(1):99−102. FANG D P, JIN L M, DONG L D, et al. Change of lignin content in wild soybeans inoculated with Phytophthora sojae [J]. Soybean Science, 2015, 34(1): 99−102.(in Chinese)
[6]	颜清上, 王连铮, 陈品三. 中国小黑豆抗源对大豆孢囊线虫4号生理小种抗病的生化反应 [J]. 作物学报, 1997, 23(5):529−537. doi: 10.3321/j.issn:0496-3490.1997.05.003 YAN Q S, WANG L Z, CHEN P S. Biochemical responses of resistance to race 4 of Heterodera glycines in Chinese black soybean [J]. Acta Agronomica Sinica, 1997, 23(5): 529−537.(in Chinese) doi: 10.3321/j.issn:0496-3490.1997.05.003
[7]	吴晓宇, 胡尚连, 曹颖, 等. 慈竹CCoAOMT基因的克隆及生物信息学分析 [J]. 南京林业大学学报(自然科学版), 2012, 36(3):17−22. WU X Y, HU S L, CAO Y, et al. Cloning of CCoAOMT gene in Neosinocalamus affinis and its bioinformatics analysis [J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2012, 36(3): 17−22.(in Chinese)
[8]	YE Z H. Association of caffeoyl coenzyme A 3-O-methyltransferase expression with lignifying tissues in several dicot plants [J]. Plant Physiology, 1997, 115(4): 1341−1350. doi: 10.1104/pp.115.4.1341
[9]	BOERJAN W, RALPH J, BAUCHER M. Lignin biosynthesis [J]. Annual Review of Plant Biology, 2003, 54: 519−546. doi: 10.1146/annurev.arplant.54.031902.134938
[10]	JOSHI C P, CHIANG V L. Conserved sequence motifs in plant S-adenosyl-L-methionine-dependent methyltransferases [J]. Plant Molecular Biology, 1998, 37(4): 663−674. doi: 10.1023/A:1006035210889
[11]	王宇光, 吕笑言, 季美超, 等. 甜菜M14品系咖啡酰辅酶A-O-甲基转移酶提高植物抗逆性功能分析 [J]. 中国农学通报, 2018, 34(34):30−35. doi: 10.11924/j.issn.1000-6850.casb18060037 WANG Y G, LV X Y, JI M C, et al. Stress tolerance improvement by BvM14-CCoAOMT gene in sugar beet M14 strain [J]. Chinese Agricultural Science Bulletin, 2018, 34(34): 30−35.(in Chinese) doi: 10.11924/j.issn.1000-6850.casb18060037
[12]	SALEKDEH G H, SIOPONGCO J, WADE L J, et al. A proteomic approach to analyzing drought- and salt-responsiveness in rice [J]. Field Crops Research, 2002, 76(2/3): 199−219.
[13]	RICCARDI F, GAZEAU P, DE V D, et al. Protein changes in response to progressive water deficit in maize. Quantitative variation and polypeptide identification [J]. Plant Physiology, 1998, 117(4): 1253−1263. doi: 10.1104/pp.117.4.1253
[14]	KÜHNL T, KOCH U, HELLER W, et al. Elicitor induced: Caffeoyl-CoA 3-O-methyltransferase from carrot cell suspension cultures [J]. Plant Science, 1989, 60(1): 21−25. doi: 10.1016/0168-9452(89)90039-3
[15]	PAKUSCH A E, KNEUSEL R E, MATERN U. S-adenosyl-l-methionine: Trans-caffeoyl-coenzyme A 3-O-methyltransferase from elicitor-treated parsley cell suspension cultures [J]. Archives of Biochemistry and Biophysics, 1989, 271(2): 488−494. doi: 10.1016/0003-9861(89)90299-3
[16]	魏建华, 宋艳茹. 木质素生物合成途径及调控的研究进展 [J]. 植物学报, 2001, 43(8):771−779. WEI J H, SONG Y R. Recent advances in study of lignin biosynthesis and manipulation [J]. Journal of Integrative Plant Biology, 2001, 43(8): 771−779.(in Chinese)
[17]	DO C T, POLLET B, THÉVENIN J, et al. Both caffeoyl Coenzyme A 3-O-methyltransferase 1 and caffeic acid O-methyltransferase 1 are involved in redundant functions for lignin, flavonoids and sinapoyl malate biosynthesis in Arabidopsis [J]. Planta, 2007, 226(5): 1117−1129. doi: 10.1007/s00425-007-0558-3
[18]	吕萌, 倪志勇, 王娟, 等. 棉花甲基化酶基因COMT和CCoAOMT的组织特异性表达分析 [J]. 核农学报, 2010, 24(4):713−719. doi: 10.11869/hnxb.2010.04.0713 LÜ M, NI Z Y, WANG J, et al. Tissue specificity express analysis of methyltransferase gene comt and ccoaomt in cotton [J]. Journal of Nuclear Agricultural Sciences, 2010, 24(4): 713−719.(in Chinese) doi: 10.11869/hnxb.2010.04.0713
[19]	吕笑言, 王宇光, 金英. 甜菜BvM14-CCoAOMT基因的克隆、表达及生物信息学分析 [J]. 黑龙江大学自然科学学报, 2018, 35(3):317−323. doi: 10.13482/j.issn1001-7011.2018.05.113 LÜ X Y, WANG Y G, JIN Y. Cloning, expression and bioinformatics analyses of BvM14-CCoAOMT gene [J]. Journal of Natural Science of Heilongjiang University, 2018, 35(3): 317−323.(in Chinese) doi: 10.13482/j.issn1001-7011.2018.05.113
[20]	林海燕, 罗勇, 陈丝, 等. 茶树CCoAOMT基因克隆及启动子的结构分析 [J]. 分子植物育种, 2019, 17(6):1804−1813. doi: 10.13271/j.mpb.017.001804 LIN H Y, LUO Y, CHEN S, et al. Cloning of CCoAOMT gene and structure analysis of its promoter in Camellia sinensis [J]. Molecular Plant Breeding, 2019, 17(6): 1804−1813.(in Chinese) doi: 10.13271/j.mpb.017.001804
[21]	刘大伟, 陈立杰, 段玉玺. 大豆胞囊线虫胁迫下不同抗性大豆杂交后代根系蛋白质组分析 [J]. 华北农学报, 2013, 28(5):29−33. doi: 10.7668/hbnxb.2013.05.005 LIU D W, CHEN L J, DUAN Y X. Proteomic analysis of soybean with different resistance differentially expressed proteins induced by Heterodera glycines [J]. Acta Agriculturae Boreali-Sinica, 2013, 28(5): 29−33.(in Chinese) doi: 10.7668/hbnxb.2013.05.005
[22]	CHEN C J, CHEN H, ZHANG Y, et al. TBtools: An integrative toolkit developed for interactive analyses of big biological data [J]. Molecular Plant, 2020, 13(8): 1194−1202. doi: 10.1016/j.molp.2020.06.009
[23]	ARTIMO P, JONNALAGEDDA M, ARNOLD K, et al. ExPASy: SIB bioinformatics resource portal [J]. Nucleic Acids Research, 2012, 40(W1): W597−W603. doi: 10.1093/nar/gks400
[24]	KYTE J, DOOLITTLE R F. A simple method for displaying the hydropathic character of a protein [J]. Journal of Molecular Biology, 1982, 157(1): 105−132. doi: 10.1016/0022-2836(82)90515-0
[25]	YU C S, LIN C J, HWANG J K. Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions [J]. Protein Science:a Publication of the Protein Society, 2004, 13(5): 1402−1406. doi: 10.1110/ps.03479604
[26]	KUMAR S, STECHER G, TAMURA K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets [J]. Molecular Biology and Evolution, 2016, 33(7): 1870−1874. doi: 10.1093/molbev/msw054
[27]	于明革, 杨洪强, 翟衡. 植物木质素及其生理学功能 [J]. 山东农业大学学报(自然科学版), 2003, 34(1):124−128. YU M G, YANG H Q, ZHAI H. Lignin and physiological function in plant [J]. Journal of Shandong Agricultural University, 2003, 34(1): 124−128.(in Chinese)
[28]	DÍAZ M L, GARBUS I, ECHENIQUE V. Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase [J]. Molecular Breeding, 2010, 26(4): 627−637. doi: 10.1007/s11032-010-9399-z
[29]	MARTZ F, MAURY S, PINÇON G, et al. cDNA cloning, substrate specificity and expression study of tobacco caffeoyl-CoA 3-O-methyltransferase, a lignin biosynthetic enzyme [J]. Plant Molecular Biology, 1998, 36(3): 427−437. doi: 10.1023/A:1005969825070
[30]	黄春琼, 刘国道, 郭安平. 反义CCoAOMT基因调控烟草木质素的生物合成 [J]. 安徽农业科学, 2008, 36(19):8026−8027, 8043. doi: 10.3969/j.issn.0517-6611.2008.19.034 HUANG C Q, LIU G D, GUO A P. Lignin biosynthesis regulated by antisense CCoAOMT gene in tobacco [J]. Journal of Anhui Agricultural Sciences, 2008, 36(19): 8026−8027, 8043.(in Chinese) doi: 10.3969/j.issn.0517-6611.2008.19.034
[31]	陈夏鑫, 陈振林, 帅良, 等. 荸荠CCoAOMT基因的克隆与表达分析 [J]. 分子植物育种, 2020, 18(20):6685−6691. doi: 10.13271/j.mpb.018.006685 CHEN X X, CHEN Z L, SHUAI L, et al. Cloning and expression analysis of CCoAOMT gene in Chinese water-chestnut [J]. Molecular Plant Breeding, 2020, 18(20): 6685−6691.(in Chinese) doi: 10.13271/j.mpb.018.006685
[32]	陈凌娜, 郭晓娟, 杨汉奇. 巨龙竹木质素合成关键基因CCoAOMT的克隆及表达分析 [J]. 植物遗传资源学报, 2019, 20(2):476−484. CHEN L N, GUO X J, YANG H Q. Cloning and expression analysis of CCoAOMT, a key gene in lignin biosynthesis of Dendrocalamus sinicus [J]. Journal of Plant Genetic Resources, 2019, 20(2): 476−484.(in Chinese)
[33]	刘鑫, 孙燕铭, 王涵, 等. 梨咖啡酰辅酶A-O-甲基转移酶基因PbCCoAOMT1的功能验证 [J]. 安徽农业大学学报, 2020, 47(5):845−850. doi: 10.13610/j.cnki.1672-352x.20200616.001 LIU X, SUN Y M, WANG H, et al. Functional verification of pear caffeoyl coenzyme A-O-methyltransferase gene Pb CCo AOMT1 [J]. Journal of Anhui Agricultural University, 2020, 47(5): 845−850.(in Chinese) doi: 10.13610/j.cnki.1672-352x.20200616.001
[34]	李元玉, 杨颖博, 张国宁, 等. 菘蓝中咖啡酰辅酶A氧甲基转移酶基因克隆与表达特征 [J]. 分子植物育种, 2022, 20(2):349−356. doi: 10.13271/j.mpb.020.000349 LI Y Y, YANG Y B, ZHANG G N, et al. Cloning and expression characteristics of caffeoyl-CoA oxygen methyltransferase gene from Isatis indigotica fort [J]. Molecular Plant Breeding, 2022, 20(2): 349−356.(in Chinese) doi: 10.13271/j.mpb.020.000349
[35]	马倩, 闫启, 张正社, 等. 紫花苜蓿CCoAOMT基因家族的鉴定、进化及表达分析 [J]. 草业学报, 2021, 30(11):144−156. doi: 10.11686/cyxb2020429 MA Q, YAN Q, ZHANG Z S, et al. Identification, evolution and expression analysis of the CCoAOMT family genes in Medicago sativa [J]. Acta Prataculturae Sinica, 2021, 30(11): 144−156.(in Chinese) doi: 10.11686/cyxb2020429