Cloning and Activity of DlAGO4 and DlAGO6 Promoters from Dimocarpus longan

CHEN Rongzhu; LAI Zhongxiong

doi:10.19303/j.issn.1008-0384.2023.05.006

Volume 38 Issue 5

May 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(5): 552-558

CHEN R Z, LAI Z X. Cloning and Activity of DlAGO4 and DlAGO6 Promoters from Dimocarpus longan [J]. Fujian Journal of Agricultural Sciences，2023，38（5）：552−558 doi: 10.19303/j.issn.1008-0384.2023.05.006

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CHEN R Z, LAI Z X. Cloning and Activity of DlAGO4 and DlAGO6 Promoters from Dimocarpus longan [J]. Fujian Journal of Agricultural Sciences，2023，38（5）：552−558 doi: 10.19303/j.issn.1008-0384.2023.05.006

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Cloning and Activity of DlAGO4 and DlAGO6 Promoters from Dimocarpus longan

doi: 10.19303/j.issn.1008-0384.2023.05.006

CHEN Rongzhu^1
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LAI Zhongxiong^{2
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1.
Zhangzhou Health Vocational College, Zhangzhou, Fujian　363000, China
2.
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2023-02-01
Accepted Date: 2023-05-11
Rev Recd Date: 2023-04-30

Available Online: 2023-07-06

Publish Date: 2023-05-28

Abstract

Abstract

Objective Argonaute (AGO) proteins play the important roles in a wide varieties of plant biological processes. To explore the cis --acting elements of DlAGO4 and DlAGO6 promoters, the expression patterns of recombinant vector of DlAGO4 and DlAGO6 promoters under different hormones treatments in longan, DlAGO4 and DlAGO6 promoters in longan were cloned and analyzed. Method Promoters of DlAGO4 and DlAGO6 from Dimocarpus longan were cloned by PCR for a bioinformatic analysis. Fusion expression vectors of the full-length promoters and GUS gene were constructed. The Agrobacterium tumefaciens-mediated transformation was performed on protocorm of tobacco leaves to observe the transient expression of the genes, while promoter activity examined by GUS histochemical staining. Result The sequence of DlAGO4 promoter was 1 514 bp and the sequence of DlAGO6 promoter was 1 784 bp. Both had the core elements of TATA- and CAAT-box as well as ababolic acid (ABA), methyl jasmonate (MeJA), and photoresponsive elements. The DlAGO4 promoter also contained salicylic acid (SA), gibberellin response, and anaerobic response regulatory elements, while the DlAGO6 promoter had auxin and circadian rhythm regulatory elements. The promoters exhibited a GUS-driving activity that was weaker than CaMV35S. The relative expression of GUS of P_DlAGO6::GUS recombinant tobacco leaf was significantly elevated by the treatment of MeJA and ABA, whereas that of P_DlAGO4::GUS recombinant tobacco leaf by SA and MeJA. Conclusion The promoters of DlAGO4 and DlAGO6 from longan were successfully cloned. As hormone-induced promoters, they could activate the GUS expression and be involved in the somatic embryo development and hormone response of plants.
- Dimocarpus longan,
- DlAGO4,
- DlAGO6,
- promoter,
- GUS activity

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References(31)

References

[1]	CHEN Y K, XU X P, CHEN X H, et al. Seed-specific gene MOTHER of FT and TFL1(MFT) involved in embryogenesis, hormones and stress responses in Dimocarpus longan lour [J]. International Journal of Molecular Sciences, 2018, 19(8): 2403. doi: 10.3390/ijms19082403
[2]	BUTLER J E F, KADONAGA J T. The RNA polymerase II core promoter: A key component in the regulation of gene expression [J]. Genes & Development, 2002, 16(20): 2583−2592.
[3]	MALLORY A, VAUCHERET H. Form, function, and regulation of ARGONAUTE proteins [J]. The Plant Cell, 2012, 22(12): 3879−3889.
[4]	CAO J Y, XU Y P, ZHAO L, et al. Tight regulation of the interaction between Brassica napus and Sclerotinia sclerotiorum at the microRNA level [J]. Plant Molecular Biology, 2016, 92(1): 39−55.
[5]	GAN D F, ZHAN M D, YANG F, et al. Expression analysis of argonaute, Dicer-like, and RNA-dependent RNA polymerase genes in cucumber (Cucumis sativus L. ) in response to abiotic stress [J]. Journal of Genetics, 2017, 96(2): 235−249. doi: 10.1007/s12041-017-0758-y
[6]	KAPOOR M, ARORA R, LAMA T, et al. Genome-wide identification, organization and phylogenetic analysis of Dicer-like, Argonaute and RNA-dependent RNA Polymerase gene families and their expression analysis during reproductive development and stress in rice [J]. BMC Genomics, 2008, 9: 451. doi: 10.1186/1471-2164-9-451
[7]	SHAO F J, LU S F. Genome-wide identification, molecular cloning, expression profiling and posttranscriptional regulation analysis of the Argonaute gene family in Salvia miltiorrhiza, an emerging model medicinal plant [J]. BMC Genomics, 2013, 14: 512. doi: 10.1186/1471-2164-14-512
[8]	QIN L, MO N, MUHAMMAD T, et al. Genome-wide analysis of DCL, AGO, and RDR gene families in pepper (Capsicum annuum L. ) [J]. International Journal of Molecular Sciences, 2018, 19(4): 1038. doi: 10.3390/ijms19041038
[9]	ZHAO H L, ZHAO K, WANG J, et al. Comprehensive analysis of dicer-like, argonaute, and RNA-dependent RNA polymerase gene families in grapevine (Vitis vinifera) [J]. Journal of Plant Growth Regulation, 2015, 34(1): 108−121. doi: 10.1007/s00344-014-9448-7
[10]	AGUSTÍN S, LUCAS D, ABELARDO V, et al. Genome-wide analysis of AGO, DCL and RDR gene families reveals RNA-directed DNA methylation is involved in fruit abscission in Citrus sinensis [J]. BMC Plant Biology, 2019, 19(1): 401. doi: 10.1186/s12870-019-1998-1
[11]	BAI M, YANG G S, CHEN W T, et al. Genome-wide identification of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in Solanum lycopersicum [J]. Gene, 2012, 501(1): 52−62. doi: 10.1016/j.gene.2012.02.009
[12]	ZHAO X, ZHENG W H, ZHONG Z H, et al. Genome-wide analysis of RNA-interference pathway in Brassica napus, and the expression profile of BnAGOs in response to Sclerotinia sclerotiorum infection [J]. European Journal of Plant Pathology, 2016, 146(3): 565−579. doi: 10.1007/s10658-016-0942-6
[13]	赵丽, 夏文强, 蔡新忠. 拟南芥AGO2的亚细胞定位分析 [J]. 浙江大学学报(农业与生命科学版), 2013, 39(1):1−10. ZHAO L, XIA W Q, CAI X Z. Identification of subcellular localization of Arabidopsis AGO2 [J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(1): 1−10.(in Chinese)
[14]	叶瑞强. 拟南芥AGO4/siRNA复合体细胞质内组装和不依赖DCL的小RNA介导DNA甲基化的机理研究[D]. 杭州: 浙江大学, 2014. YE R Q. Cytoplasmic assembly of AGO4/siRNA complexes and DNA methylation guided by DCL-independent sRNAs in Arabidopsis[D]. Hangzhou: Zhejiang University, 2014. (in Chinese)
[15]	HERNÁNDEZ-LAGANA E, RODRÍGUEZ-LEAL D, LÚA J, et al. A multigenic network of ARGONAUTE4 clade members controls early megaspore formation in Arabidopsis [J]. Genetics, 2016, 204(3): 1045−1056. doi: 10.1534/genetics.116.188151
[16]	MCCUE A D, PANDA K, NUTHIKATTU S, et al. ARGONAUTE 6 bridges transposable element mRNA-derived siRNAs to the establishment of DNA methylation [J]. The EMBO Journal, 2015, 34(1): 20−35. doi: 10.15252/embj.201489499
[17]	LIN Y L, MIN J M, LAI R L, et al. Genome-wide sequencing of Longan (Dimocarpus longan Lour. ) provides insights into molecular basis of its polyphenol-rich characteristics [J]. GigaScience, 2017, 6(5): gix023.
[18]	CHEN R Z, SHEN X, ZHANG S T, et al. Genome-wide identification and expression analysis of Argonaute gene family from Longan embryogenic callus [J]. Journal of Integrative Agriculture, 2021, 20(8): 2138−2155. doi: 10.1016/S2095-3119(20)63313-5
[19]	陈荣珠, 申序, 林美珍, 等. 龙眼体胚发生过程中DlAGO4基因的克隆及表达分析 [J]. 西北植物学报, 2020, 40(5):747−755. CHEN R Z, SHEN X, LIN M Z, et al. Cloning and expression analysis of DlAGO4 gene from embryogenic callus in Dimocarpus longan lour [J]. Acta Botanica Boreali-Occidentalia Sinica, 2020, 40(5): 747−755.(in Chinese)
[20]	田奇琳. 龙眼胚性培养物DlRan3A和DlRan3B基因的功能分析[D]. 福州: 福建农林大学, 2017. TIAN Q L. Characterization of DlRan3A and DlRan3B gene from embryogenic cultures in Dimocarpus longan lour[D]. Fuzhou: Fujian Agriculture and Forestry University, 2017. (in Chinese)
[21]	曾丽兰. 龙眼胚性愈伤组织SOD的表达分析及启动子功能鉴定[D]. 福州: 福建农林大学, 2013. ZENG L L. Characterization of SOD promoters and expression analysis of SOD genes of the embryogenic callus in Dimocarpus longan lour[D]. Fuzhou: Fujian Agriculture and Forestry University, 2013. (in Chinese)
[22]	李豆, 苏功博, 胡晓晴, 等. 白桦BpSPL6基因启动子的克隆及表达分析 [J]. 北京林业大学学报, 2022, 44(2):1−10. doi: 10.12171/j.1000-1522.20200174 LI D, SU G B, HU X Q, et al. Cloning and expression analysis of BpSPL6 promoter from Betula platyphylla [J]. Journal of Beijing Forestry University, 2022, 44(2): 1−10.(in Chinese) doi: 10.12171/j.1000-1522.20200174
[23]	魏灵敏, 温少莹, 马际凯, 等. 北美鹅掌楸LtAGO1基因的克隆、表达及其启动子分析 [J]. 广西植物, 2022, 42(8):1402−1416. doi: 10.11931/guihaia.gxzw202102030 WEI L M, WEN S Y, MA J K, et al. Cloning, expression and promoter analysis of LtAGO1 from Liriodendron tulipifera [J]. Guihaia, 2022, 42(8): 1402−1416.(in Chinese) doi: 10.11931/guihaia.gxzw202102030
[24]	陈荣珠, 王小平, 申序, 等. 龙眼DlAGO1基因启动子的克隆与表达 [J]. 应用与环境生物学报, 2022, 28(2):485−490. CHEN R Z, WANG X P, SHEN X, et al. Cloning and expression analysis of Dl AGO1 promoter in Dimocarpus longan [J]. Chinese Journal of Applied and Environmental Biology, 2022, 28(2): 485−490.(in Chinese)
[25]	陈晓慧, 曾丽兰, 徐小萍, 等. 龙眼Mn-SOD基因的表达及其启动子功能分析 [J]. 热带作物学报, 2018, 39(5):913−919. CHEN X H, ZENG L L, XU X P, et al. Expression of Mn-SOD and functional analysis of its promoter in Dimocarpus longan lour [J]. Chinese Journal of Tropical Crops, 2018, 39(5): 913−919.(in Chinese)
[26]	田奇琳, 林玉玲, 赖钟雄, 等. 龙眼胚性愈伤组织DlRan3A和DlRan3B基因启动子的克隆及其生物信息学分析 [J]. 热带作物学报, 2014, 35(1):82−89. TIAN Q L, LIN Y L, LAI Z X, et al. Cloning and bioinformatic analysis of the promoters of DlRan3A and DlRan3B from embryogenic callus in Dimocarpus longan [J]. Chinese Journal of Tropical Crops, 2014, 35(1): 82−89.(in Chinese)
[27]	BOHMERT K. AGO1 defines a novel locus of Arabidopsis controlling leaf development [J]. The EMBO Journal, 1998, 17(1): 170−180. doi: 10.1093/emboj/17.1.170
[28]	LIU C, XIN Y, XU L, et al. Arabidopsis ARGONAUTE 1 binds chromatin to promote gene transcription in response to hormones and stresses [J]. Developmental Cell, 2018, 44(3): 348−361.e7. doi: 10.1016/j.devcel.2017.12.002
[29]	YANG Y, ZHONG J, OUYANG Y D, et al. The integrative expression and co-expression analysis of the AGO gene family in rice [J]. Gene, 2013, 528(2): 221−235. doi: 10.1016/j.gene.2013.07.002
[30]	黄伦增, 许云泓, 孟凡文, 等. 杨树PtTST3基因启动子克隆及表达活性分析 [J]. 分子植物育种, 2022, 20(17):5649−5657. HUANG L Z, XU Y H, MENG F W, et al. Cloning and expression activity analysis of PtTST3 gene promoter in poplar [J]. Molecular Plant Breeding, 2022, 20(17): 5649−5657.(in Chinese)
[31]	谢牧洪, 李文凯, 张昭龙, 等. 核桃JrTT1-1启动子不同长度片段响应干旱的活性分析 [J]. 北京林业大学学报, 2022, 44(8):31−38. XIE M H, LI W K, ZHANG Z L, et al. Activity analysis of different length fragments of walnut JrTT1-1 promoter in response to drought stress [J]. Journal of Beijing Forestry University, 2022, 44(8): 31−38.(in Chinese)