Glyphosate-tolerant Maize Plants Generated by Pollen Tube Pathway Method

LI Xianglong; ZHENG Dengyu; ZHANG Chun; ZHANG Zhongbao; WU Zhongyi

doi:10.19303/j.issn.1008-0384.2023.05.002

Volume 38 Issue 5

May 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(5): 524-529

LI X L, ZHENG D Y, ZHANG C, et al. Glyphosate-tolerant Maize Plants Generated by Pollen Tube Pathway Method [J]. Fujian Journal of Agricultural Sciences，2023，38（5）：524−529 doi: 10.19303/j.issn.1008-0384.2023.05.002

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LI X L, ZHENG D Y, ZHANG C, et al. Glyphosate-tolerant Maize Plants Generated by Pollen Tube Pathway Method [J]. Fujian Journal of Agricultural Sciences，2023，38（5）：524−529 doi: 10.19303/j.issn.1008-0384.2023.05.002

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Glyphosate-tolerant Maize Plants Generated by Pollen Tube Pathway Method

doi: 10.19303/j.issn.1008-0384.2023.05.002

Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing　100097, China

Received Date: 2023-03-30
Rev Recd Date: 2023-04-15

Available Online: 2023-05-24

Publish Date: 2023-05-28

Abstract

Abstract

Objective Stable transgenic glyphosate-tolerant strains of maize were obtained by screening T₀-T₃ generations of the genetically modified plants for the presence of EPSPS after an artificial exposure to glyphosate in the field. Method The gene, EPSPS, responsible for a maize plant to resist the herbicide was transferred into the excellent inbred line Jing 92 by means of the pollen tube pathway method. Expression of the introduced gene in maize was verified by molecular detection after exposing to glyphosate in the field. Result Fifteen resistant plants from T₀ generation of the transgenic maize plants were identified after a treatment of 200 mg·L⁻¹ glyphosate, and 10 by PCR. The genetic transformation rate was 1.03%. The identified plants were further scrutinized to eliminate non-resistant-gene-carriers. The proportion of confirmed carriers among the plants increased from the T₁ to the T₃ generation. Out of 5 strains in T₃ generation, K3 and K8 were confirmed to have positively integrated EPSPS and be hereditarily stable. A test strip functional analysis on the two strains further verified the presence of the target gene. Conclusion The K3 and K8 transgenic lines identified by this study could plausibly be used to breed glyphosate-tolerant maize plants for cultivation.
- Maize,
- pollen tube pathway method,
- EPSPS,
- glyphosate-tolerance

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References(17)

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