Effects of Chemical Fertilizer Reduction with Humic Acid Addition on Carbon and Nitrogen Metabolism of <i>Zea mays</i> under Straw-returning Cultivation

GUO Wei; DAN Wuxia; MA Chuanfang; SUN Haiyan

doi:10.19303/j.issn.1008-0384.2023.04.012

Volume 38 Issue 4

Apr. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(4): 475-484

GUO W, DAN W X, MA C F, et al. Effects of Chemical Fertilizer Reduction with Humic Acid Addition on Carbon and Nitrogen Metabolism of Zea mays under Straw-returning Cultivation [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：475−484 doi: 10.19303/j.issn.1008-0384.2023.04.012

Citation:

GUO W, DAN W X, MA C F, et al. Effects of Chemical Fertilizer Reduction with Humic Acid Addition on Carbon and Nitrogen Metabolism of Zea mays under Straw-returning Cultivation [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：475−484 doi: 10.19303/j.issn.1008-0384.2023.04.012

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Effects of Chemical Fertilizer Reduction with Humic Acid Addition on Carbon and Nitrogen Metabolism of Zea mays under Straw-returning Cultivation

doi: 10.19303/j.issn.1008-0384.2023.04.012

GUO Wei^{1, 2
,},
DAN Wuxia¹,
MA Chuanfang¹,
SUN Haiyan^{1, 3}

1.
College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang　163319, China
2.
Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and Germplasm Improvement, Daqing, Heilongjiang　163319, China
3.
Key Laboratory of Low Carbon Green Agriculture in Northeast Plain，Ministry of Agriculture and Rural Affairs, Daqing, Heilongjiang　163319, China

Received Date: 2022-08-21
Rev Recd Date: 2023-03-03

Available Online: 2023-05-09

Publish Date: 2023-04-28

Abstract

Abstract

Objective Effects on plant metabolism of the widely practiced utilizing spent straws for fertilization in northeastern China corn fields were studied. Method In a field experimentation, application of either the conventional chemical fertilization (CK) or 15% reduction of chemical fertilizer with an addition of humic acid (HA), spent straws (SR), or both HA and SR (SRH) was implemented on a black soil lot of Zea mays. Physiological responses including carbon and nitrogen metabolisms to the treatments of current-season corn plants were monitored. Result The replacement of chemical fertilizer by SR enhanced the phosphoenolpyruvate carboxylase (PEPC) activity, that by HA decreased the 1,5-ribulose bisphosphate carboxylase (Rubisco) and PEPC activities, and that by SRH interacted on PEPC in the corn leaves. Although SR decreased, HA increased the sucrose phosphate synthase (SPS) activity. Both SR and HA (SRH) treatment raised the activities of sucrose synthase (SS), and combined use of HA and HA(SRH) increased the acid invertase (AI), and neutral invertase (NI) promoting the synthesis of reducing sugars that resulted in an increased total soluble sugar (TSS) and starch synthesis. SR had no significant effect, but HA had a positive effect on key enzymes involving the nitrogen metabolism of the plant. Conclusion With a 15% reduction on chemical fertilizer, addition of SRH boosted the photosynthetic efficiency and storage of photosynthetic products of corn leaves without any significant effect on the nitrogen metabolism of the plant.
- Straw-returning,
- chemical fertilizer reduction,
- humic acid,
- carbon and nitrogen metabolisms

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References(29)

References

[1]	XIN L J. Chemical fertilizer rate, use efficiency and reduction of cereal crops in China, 1998–2018 [J]. Journal of Geographical Sciences, 2022, 32(1): 65−78. doi: 10.1007/s11442-022-1936-2
[2]	王囡囡, 朱凤莉, 张春峰, 等. 不同秸秆还田方式对白浆土土壤养分及大豆产量的影响 [J]. 中国土壤与肥料, 2016(1):94−97. doi: 10.11838/sfsc.20160116 WANG N N, ZHU F L, ZHANG C F, et al. Effect of different returning method of straw to soil on soil chemical property and soybean yield [J]. Soil and Fertilizer Sciences in China, 2016(1): 94−97.(in Chinese) doi: 10.11838/sfsc.20160116
[3]	王宇先, 孙士明, 徐莹莹, 等. 黑龙江省半干旱地区生长季玉米秸秆腐解特征研究 [J]. 黑龙江农业科学, 2020(9):46−48. doi: 10.11942/j.issn1002-2767.2020.09.0046 WANG Y X, SUN S M, XU Y Y, et al. Study on decomposition characteristics of maize straw in semi-arid region of Heilongjiang Province during growing season [J]. Heilongjiang Agricultural Sciences, 2020(9): 46−48.(in Chinese) doi: 10.11942/j.issn1002-2767.2020.09.0046
[4]	张水勤, 袁亮, 林治安, 等. 腐植酸促进植物生长的机理研究进展[J]. 植物营养与肥料学报, 2017, 23(4): 1065-1076. ZHANG S Q, YUAN L, LIN Z A, et al. Advances in humic acid for promoting plant growth and its mechanism[J]. Plant Nutrition and Fertilizer Science, 2017, 23(4)4: 1065-1076. (in Chinese)
[5]	SHAHBAZ M, KUMAR A, KUZYAKOV Y, et al. Priming effects induced by glucose and decaying plant residues on SOM decomposition: A three-source ¹³C/¹⁴C partitioning study [J]. Soil Biology and Biochemistry, 2018, 121: 138−146. doi: 10.1016/j.soilbio.2018.03.004
[6]	韦安培, 丁文超, 胡恒宇, 等. 耕作方式及秸秆还田对土壤性质、微生物碳源代谢及小麦产量的影响 [J]. 干旱地区农业研究, 2019, 37(6):145−152. doi: 10.7606/j.issn.1000-7601.2019.06.21 WEI A P, DING W C, HU H Y, et al. Effects of tillage methods and straw return on soil properties, metabolism of microbial carbon source and wheat yield [J]. Agricultural Research in the Arid Areas, 2019, 37(6): 145−152.(in Chinese) doi: 10.7606/j.issn.1000-7601.2019.06.21
[7]	孔雨, 刘格彤, 张颖, 等. 秸秆还田对麦田碳氮代谢生理特性的影响 [J]. 焦作大学学报, 2013, 27(3):82−83. doi: 10.3969/j.issn.1008-7257.2013.03.028 KONG Y, LIU G T, ZHANG Y, et al. Effects of straw returning on physiological characteristics of carbon and nitrogen metabolism in wheat field [J]. Journal of Jiaozuo University, 2013, 27(3): 82−83.(in Chinese) doi: 10.3969/j.issn.1008-7257.2013.03.028
[8]	王娟, 张丽君, 姚槐应. 添加秸秆和黑炭对水稻土碳氮转化及土壤微生物代谢图谱的影响 [J]. 中国水稻科学, 2013, 27(1):97−104. doi: 10.3969/j.issn.1001-7216.2013.01.014 WANG J, ZHANG L J, YAO H Y. Effects of straw and black carbon addition on C-N transformation and microbial metabolism profile in paddy soil [J]. Chinese Journal of Rice Science, 2013, 27(1): 97−104.(in Chinese) doi: 10.3969/j.issn.1001-7216.2013.01.014
[9]	宋蒙亚, 李忠佩, 刘明, 等. 不同有机物料组合对土壤养分和生化性状的影响 [J]. 中国农业科学, 2013, 46(17):3594−3603. doi: 10.3864/j.issn.0578-1752.2013.17.008 SONG M Y, LI Z P, LIU M, et al. Effects of mixtures of different organic materials on soil nutrient content and soil biochemical characteristics [J]. Scientia Agricultura Sinica, 2013, 46(17): 3594−3603.(in Chinese) doi: 10.3864/j.issn.0578-1752.2013.17.008
[10]	李雪利, 叶协锋, 顾建国, 等. 土壤C/N比对烤烟碳氮代谢关键酶活性和烟叶品质影响的研究 [J]. 中国烟草学报, 2011, 17(3):32−36. doi: 10.3969/j.issn.1004-5708.2011.03.007 LI X L, YE X F, GU J G, et al. Effect of soil C/N ratio on activity of key enzymes involved in carbon and nitrogen metabolism and quality of flue-cured tobacco leaves [J]. Acta Tabacaria Sinica, 2011, 17(3): 32−36.(in Chinese) doi: 10.3969/j.issn.1004-5708.2011.03.007
[11]	CRUZ J L, MOSQUIM P R, PELACANI C R, et al. Carbon partitioning and assimilation as affected by nitrogen deficiency in cassava [J]. Photosynthetica, 2003, 41(2): 201−207. doi: 10.1023/B:PHOT.0000011952.77340.53
[12]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[13]	任娇, 于寒, 吴春胜, 等. 不同秸秆还田方式下玉米叶片光合、荧光特性及水分利用率的比较 [J]. 分子植物育种, 2017, 15(12):5241−5247. doi: 10.13271/j.mpb.015.005241 REN J, YU H, WU C S, et al. Comparison of photosynthesis, fluorescence characteristics and water use efficiency of maize leaves under different straw returning methods [J]. Molecular Plant Breeding, 2017, 15(12): 5241−5247.(in Chinese) doi: 10.13271/j.mpb.015.005241
[14]	李英浩, 刘景辉, 赵宝平, 等. 干旱胁迫下腐植酸对燕麦叶片非结构性碳水化合物代谢的影响 [J]. 西北植物学报, 2020, 40(12):2101−2107. doi: 10.7606/j.issn.1000-4025.2020.12.2101 LI Y H, LIU J H, ZHAO B P, et al. Effects of humic acid on metabolism of non-structural carbohydrates in oat leaves under drought stress [J]. Acta Botanica Boreali-Occidentalia Sinica, 2020, 40(12): 2101−2107.(in Chinese) doi: 10.7606/j.issn.1000-4025.2020.12.2101
[15]	BATLLE-AGUILAR J, BROVELLI A, PORPORATO A, et al. Modelling soil carbon and nitrogen cycles during land use change. A review [J]. Agronomy for Sustainable Development, 2011, 31(2): 251−274. doi: 10.1051/agro/2010007
[16]	庄振东, 李絮花. 腐植酸氮肥对玉米产量、氮肥利用及氮肥损失的影响 [J]. 植物营养与肥料学报, 2016, 22(5):1232−1239. ZHUANG Z D, LI X H. Effects of humic acid nitrogen fertilization on corn yield, nitrogen utilization and nitrogen loss [J]. Journal of Plant Nutrition and Fertilizers, 2016, 22(5): 1232−1239.(in Chinese)
[17]	谷端银, 王秀峰, 高俊杰, 等. 纯化腐植酸对氮胁迫下黄瓜幼苗生长和氮代谢的影响 [J]. 应用生态学报, 2018, 29(8):2575−2582. doi: 10.13287/j.1001-9332.201808.029 GU D Y, WANG X F, GAO J J, et al. Effects of purified humic acid on the growth and nitrogen metabolism of cucumber seedlings under nitrogen stress [J]. Chinese Journal of Applied Ecology, 2018, 29(8): 2575−2582.(in Chinese) doi: 10.13287/j.1001-9332.201808.029
[18]	LIU J, ZHONG Y, JIA X Y, et al. Wheat straw decomposition patterns and control factors under nitrogen fertilization [J]. Journal of Soil Science and Plant Nutrition, 2021, 21(4): 3110−3121. doi: 10.1007/s42729-021-00592-z
[19]	NARDI S, PIZZEGHELLO D, GESSA C, et al. A low molecular weight humic fraction on nitrate uptake and protein synthesis in maize seedlings [J]. Soil Biology and Biochemistry, 2000, 32(3): 415−419. doi: 10.1016/S0038-0717(99)00168-6
[20]	裴丽珍, 陈远学, 张雯雯, 等. 有机物料还田对夏玉米穗位叶光合性能及氮代谢的影响 [J]. 作物学报, 2022, 48(8):2115−2124. PEI L Z, CHEN Y X, ZHANG W W, et al. Effects of organic material returned on photosynthetic performance and nitro-Gen metabolism of ear leaf in summer maize [J]. Acta Agronomica Sinica, 2022, 48(8): 2115−2124.(in Chinese)
[21]	袁天佑, 王俊忠, 冀建华, 等. 施用腐植酸对夏玉米产量、氮素吸收及氮肥利用率的影响 [J]. 核农学报, 2017, 31(4):794−802. doi: 10.11869/j.issn.100-8551.2017.04.0794 YUAN T Y, WANG J Z, JI J H, et al. Effects of application humic acid on yield, nitrogen absorption and nitrogen use efficiency of summer maize [J]. Journal of Nuclear Agricultural Sciences, 2017, 31(4): 794−802.(in Chinese) doi: 10.11869/j.issn.100-8551.2017.04.0794
[22]	朱祖祥. 从绿肥的起爆效应探讨它的肥效机制及其在施用上的若干问题 [J]. 浙江农业科学, 1963, 4(3):104−109. doi: 10.16178/j.issn.0528-9017.1963.03.002 ZHU Z X. Discussing the fertilizer effect mechanism of green manure and its application problems [J]. Journal of Zhejiang Agricultural Sciences, 1963, 4(3): 104−109.(in Chinese) doi: 10.16178/j.issn.0528-9017.1963.03.002
[23]	WILD B, LI J, PIHLBLAD J, et al. Decoupling of priming and microbial N mining during a short-term soil incubation [J]. Soil Biology and Biochemistry, 2019, 129: 71−79. doi: 10.1016/j.soilbio.2018.11.014
[24]	MAZZILLI S R, KEMANIAN A R, ERNST O R, et al. Priming of soil organic carbon decomposition induced by corn compared to soybean crops [J]. Soil Biology and Biochemistry, 2014, 75: 273−281. doi: 10.1016/j.soilbio.2014.04.005
[25]	MOORHEAD D L, SINSABAUGH R L. A theoretical model of litter decay and microbial interaction [J]. Ecological Monographs, 2006, 76(2): 151−174. doi: 10.1890/0012-9615(2006)076[0151:ATMOLD]2.0.CO;2
[26]	DONG L H, CÓRDOVA-KREYLOS A L, YANG J S, et al. Humic acids buffer the effects of urea on soil ammonia oxidizers and potential nitrification [J]. Soil Biology and Biochemistry, 2009, 41(8): 1612−1621. doi: 10.1016/j.soilbio.2009.04.023
[27]	REEZA A A, AHMED O H, MAJID N M N A, et al. Reducing ammonia loss from urea by mixing with humic and fulvic acids isolated from coal [J]. American Journal of Environmental Sciences, 2009, 5(3): 420−426. doi: 10.3844/ajessp.2009.420.426
[28]	GARCÍA A C, BERBARA R L L, FARÍAS L P, et al. Humic acids of vermicompost as an ecological pathway to increase resistance of rice seedlings to water stress [J]. African Journal of Biotechnology, 2012, 11(13): 3125−3134.
[29]	CANELLAS L P, PICCOLO A, DOBBSS L B, et al. Chemical composition and bioactivity properties of size-fractions separated from a vermicompost humic acid [J]. Chemosphere, 2010, 78(4): 457−466. doi: 10.1016/j.chemosphere.2009.10.018