A RT-PCR Method for Detecting Bovine Rotavirus

  Objective   A method for bovine rotavirus (BRV) detection was developed to determine the current epidemiological status in Fujian province.

  Method   A pair of specific primers was designed based on the BRV genome from GenBank using Oligo7.0. Reaction conditions of the RT-PCR method were optimized for the detection. Specificity, sensitivity, and repeatability of the assay were verified on 22 clinical samples.

  Result   The newly developed method amplified only the specific fragment of BRV, not any of other common pathogens of livestock and poultry. A sensitivity of 6.86×10⁵ copies·μL⁻¹ was achieved. And, on the 22 clinical samples, 12 were tested positive at a rate of 54.55%.

  Conclusion   The established RT-PCR method for BRV detection displayed high specificity, sensitivity and repeatability, which could be applied for clinical diagnosis and epidemiological investigation.