The Community Structure and Diversity Characteristics of Rhizosphere Bacteria and Endophytic Bacteria in <i>Phyllostachys edulis</i> under Annual Growth Characteristics (On and Off Years)

YUAN Zongsheng; LIU Fang; ZHANG Jinfeng; CHEN Honghua; GUO Jianfang; ZENG Zhihao; WANG Sifan; WANG Yingzi; PAN Hui

doi:10.19303/j.issn.1008-0384.2023.02.012

Volume 38 Issue 2

Feb. 2023

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(2): 220-228

YUAN Z S, LIU F, ZHANG J F, et al. The Community Structure and Diversity Characteristics of Rhizosphere Bacteria and Endophytic Bacteria in Phyllostachys edulis under Annual Growth Characteristics (On and Off Years) [J]. Fujian Journal of Agricultural Sciences，2023，38（2）：220−228 doi: 10.19303/j.issn.1008-0384.2023.02.012

Citation:

YUAN Z S, LIU F, ZHANG J F, et al. The Community Structure and Diversity Characteristics of Rhizosphere Bacteria and Endophytic Bacteria in Phyllostachys edulis under Annual Growth Characteristics (On and Off Years) [J]. Fujian Journal of Agricultural Sciences，2023，38（2）：220−228 doi: 10.19303/j.issn.1008-0384.2023.02.012

Citation:

PDF( 2803 KB)

The Community Structure and Diversity Characteristics of Rhizosphere Bacteria and Endophytic Bacteria in Phyllostachys edulis under Annual Growth Characteristics (On and Off Years)

doi: 10.19303/j.issn.1008-0384.2023.02.012

1.
College of Geography and Oceanography, Minjiang University, Fuzhou, Fujian　350108, China
2.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002,China
3.
Yong'an State-owned Forest Farm, Sanming, Fujian　366099, China
4.
College of Future Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
5.
Nature Reserve Center of Fuzhou Forestry Bureau, Fuzhou, Fujian　350005, China

Received Date: 2022-10-09
Rev Recd Date: 2022-12-21
Publish Date: 2023-02-28

Abstract

Abstract

Objective To study the diversity and structural differences of rhizosphere bacteria and endophytic bacterial communities in Phyllostachys edulis forests during on and off years. Methods Samples of rhizomes and rhizomes roots as well as rhizosphere soils of degree I, degree II and degree IV P. edulis and non-rhizosphere soils were collected in the on and off years. Genomic DNA was extracted from samples, and Illumina high-throughput sequencing technology was used to analyze the diversity of rhizosphere bacteria and endophytic bacterial communities in P. edulis. Results A total of 31 phyla, 49 classes, 108 orders, 212 families, and 472 genera were identified. The dominant phyla in the on-year P. edulis rhizomes and rhizomes roots were α-Amastigotes, and the dominant order Rhizobia. In the off-year specimens, the dominant phylum was γ-Amastigotes, and the dominant order Bacillariophyceae. At phylum level, the abundance of Actinobacteria was higher in the on-year than in off-year rhizomes; and those of Acidobacteria and Methylobacteria were greater in the on-year than in the off-year rhizomes roots; and those of Firmicutes and Bacteroidetes were less in the on-year than in the off-year rhizomes roots. At class and order levels, the dominants included Frankiaceae and Rhizobia of α-Amastigotes in the on-year rhizomes and rhizomes roots as compared with the off-year samples. At the family level, the abundance of Flavobacteriaceae in the rhizomes root of the on-year was greater than that in the off-year samples. At the genus level, the abundance of Bradyrhizobium in the rhizomes root of on-year was greater than that in off-year. However, the abundance of Burkholderiaceae was lower of on-year than off-year. The rhizosphere soil at the forest in either on or off years did not differ significantly on bacterial diversity, but it was higher on the diversity and richness than the non-rhizosphere soil. Conclusion The rhizosphere bacterial community at a P. edulis forest appeared to be more diverse than the non-rhizosphere, although the diversity was not significantly altered between the years of on and off on the P. edulis growth. The dominant bacteria in the rhizomes and rhizomes roots of the plants differed significantly during the on and off years.
- Phyllostachys edulis forest,
- on and off year,
- bacterial community,
- diversity

FullText(HTML)

References(31)

References

[1]	PENG Z H, LU Y, LI L B, et al. The draft genome of the fast-growing non-timber forest species moso bamboo (Phyllostachys heterocycla) [J]. Nature Genetics, 2013, 45(4): 456−461. doi: 10.1038/ng.2569
[2]	LONGWEI, LI N, LU D S, et al. Mapping Moso bamboo forest and its on-year and off-year distribution in a subtropical region using time-series Sentinel-2 and Landsat 8 data [J]. Remote Sensing of Environment, 2019, 231: 111265. doi: 10.1016/j.rse.2019.111265
[3]	ZHOU Y F, ZHOU G M, DU H Q, et al. Biotic and abiotic influences on monthly variation in carbon fluxes in on-year and off-year Moso bamboo forest [J]. Trees, 2019, 33(1): 153−169. doi: 10.1007/s00468-018-1765-1
[4]	SHELAKE R M, PRAMANIK D, KIM J Y. Exploration of plant-microbe interactions for sustainable agriculture in CRISPR era [J]. Microorganisms, 2019, 7(8): 269. doi: 10.3390/microorganisms7080269
[5]	MÜLLER D B, VOGEL C, BAI Y, et al. The plant microbiota: Systems-level insights and perspectives [J]. Annual Review of Genetics, 2016, 50: 211−234. doi: 10.1146/annurev-genet-120215-034952
[6]	MA B, WANG H Z, DSOUZA M, et al. Geographic patterns of co-occurrence network topological features for soil microbiota at continental scale in Eastern China [J]. The ISME Journal, 2016, 10(8): 1891−1901. doi: 10.1038/ismej.2015.261
[7]	KLOEPPER J W, LEONG J, TEINTZE M, et al . Enhanced plant growth by siderophores produced by plant growth -promothing rhizobacteria [J]. Nature, 1980, 286 (5776): 885−886 . doi: 10.1038/286885a0
[8]	BULGARELLI D, SCHLAEPPI K, SPAEPEN S, et al . Sturcture and functions of the bacterial microbiota of plant [J]. Annual Review of Plant Biology, 2013, 64 : 807−838. doi: 10.1146/annurev-arplant-050312-120106
[9]	吴良如,萧江华. 大小年毛竹林中内源激素节律变化特征的研究 [J]. 竹子研究汇刊,1998, 1998, 17(1):24−30. LIANGU W, JIANGHUA XIAO. Study on Dynamic Characteristics of Eudogenous Phytohormone in On-and-Off Year Bamboo (Phyllost achys Heterocycles Var. Pubescens) Grove [J]. Journal of Bamboo Research, 1998, 17(1): 24−30.(in Chinese)
[10]	EDGAR R C. UPARSE: Highly accurate OTU sequences from microbial amplicon reads [J]. Nature Methods, 2013, 10(10): 996−998. doi: 10.1038/nmeth.2604
[11]	LIN X C, CHOW T Y, CHEN H H, et al. Understanding bamboo flowering based on large-scale analysis of expressed sequence tags [J]. Genetics and Molecular Research:GMR, 2010, 9(2): 1085−1093. doi: 10.4238/vol9-2gmr804
[12]	SCHLOSS P D, WESTCOTT S L, RYABIN T, et al. Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities [J]. Applied and Environmental Microbiology, 2009, 75(23): 7537−7541. doi: 10.1128/AEM.01541-09
[13]	ISAGI Y, SHIMADA K, KUSHIMA H, et al. Clonal structure and flowering traits of a bamboo[Phyllostachys pubescens (Mazel) Ohwi]stand grown from a simultaneous flowering as revealed by AFLP analysis [J]. Molecular Ecology, 2004, 13(7): 2017−2021. doi: 10.1111/j.1365-294X.2004.02197.x
[14]	BAIS H P, WEIR T L, PERRY L G, et al. The role of root exudates in rhizosphere interactions with plants and other organisms [J]. Annual Review of Plant Biology, 2006, 57: 233−266. doi: 10.1146/annurev.arplant.57.032905.105159
[15]	REINHOLD-HUREK B, BÜNGER W, BURBANO C S, et al. Roots shaping their microbiome: Global hotspots for microbial activity [J]. Annual Review of Phytopathology, 2015, 53: 403−424. doi: 10.1146/annurev-phyto-082712-102342
[16]	COLEMAN-DERR D, DESGARENNES D, FONSECA-GARCIA C, et al. Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species [J]. The New Phytologist, 2016, 209(2): 798−811. doi: 10.1111/nph.13697
[17]	XUAN D T, GUONG V T, ROSLING A, et al. Different crop rotation systems as drivers of change in soil bacterial community structure and yield of rice, Oryza sativa [J]. Biology and Fertility of Soils, 2012, 48(2): 217−225. doi: 10.1007/s00374-011-0618-5
[18]	XIANGZHEN, LI,. Dynamics of the bacterial community structure in the rhizosphere of a maize cultivar [J]. Soil Biology and Biochemistry, 2014, 68: 392−401. doi: 10.1016/j.soilbio.2013.10.017
[19]	SHI Y H, PAN Y S, XIANG L, et al. Assembly of rhizosphere microbial communities in Artemisia annua: Recruitment of plant growth-promoting microorganisms and inter-Kingdom interactions between bacteria and fungi [J]. Plant and Soil, 2022, 470(1): 127−139.
[20]	BERENDSEN R L, PIETERSE C M J, BAKKER P A H M. The rhizosphere microbiome and plant health [J]. Trends in Plant Science, 2012, 17(8): 478−486. doi: 10.1016/j.tplants.2012.04.001
[21]	HENNING S M, YANG J P, SHAO P, et al. Health benefit of vegetable/fruit juice-based diet: Role of microbiome [J]. Scientific Reports, 2017, 7: 2167. doi: 10.1038/s41598-017-02200-6
[22]	PENG G X, ZHANG W, LUO H F, et al. Enterobacter oryzae sp. nov. , a nitrogen-fixing bacterium isolated from the wild rice species Oryza latifolia[J]. International Journal of Systematic and Evolutionary Microbiology, 2009, 59(Pt 7): 1650-1655.
[23]	BOTHE H. Biology of the Nitrogen Cycle[M]. Amsterdam: Elsevier Science Ltd, 2007: 147-163.
[24]	OROZCO-MOSQUEDA M D C, ROCHA-GRANADOS M D C, GLICK B R, et al. Microbiome engineering to improve biocontrol and plant growth-promoting mechanisms [J]. Microbiological Research, 2018, 208: 25−31. doi: 10.1016/j.micres.2018.01.005
[25]	STÉPHANE, COMPANT C, SESSITSCH A. Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization [J]. Soil Biology and Biochemistry, 2010, 42(5): 669−678. doi: 10.1016/j.soilbio.2009.11.024
[26]	张爱梅, 殷一然, 孙坤. 沙棘属植物弗兰克氏菌研究进展 [J]. 微生物学通报, 2020, 47(11):3933−3944. doi: 10.13344/j.microbiol.china.200427 ZHANG A M, YIN Y R, SUN K. Research progress in Frankia spp. associated with Hippophae L [J]. Microbiology China, 2020, 47(11): 3933−3944.(in Chinese) doi: 10.13344/j.microbiol.china.200427
[27]	DIAGNE N, ARUMUGAM K, NGOM M, et al. Use of Frankia and actinorhizal plants for degraded lands reclamation [J]. BioMed Research International, 2013, 2013: 948258.
[28]	黄瑞林, 张娜, 孙波, 等. 典型农田根际土壤伯克霍尔德氏菌群落结构及其多样性 [J]. 土壤学报, 2020, 57(4):975−985. doi: 10.11766/trxb201901040008 HUANG R L, ZHANG N, SUN B, et al. Community structure of burkholderiales and its diversity in typical maize rhizosphere soil [J]. Acta Pedologica Sinica, 2020, 57(4): 975−985.(in Chinese) doi: 10.11766/trxb201901040008
[29]	SIJAM K, DIKIN A. Biochemical and physiological characterization of Burkholderia cepacia as biological control agent [J]. International Journal of Agriculture & Biology, 2005, 7(3): 385−388.
[30]	HARDOIM P R, VAN OVERBEEK L S, ELSAS J D. Properties of bacterial endophytes and their proposed role in plant growth [J]. Trends in Microbiology, 2008, 16(10): 463−471. doi: 10.1016/j.tim.2008.07.008
[31]	LEBEIS S L. The potential for give and take in plant-microbiome relationships [J]. Frontiers in Plant Science, 2014, 5: 287.