Morphological and SRAP Markers-based Genetic Diversity Determination on <i>Phaseolus lunatus</i> L. Germplasms

GUO Yuanzhen; HUANG Qiang; YE Xinru; CHEN Zhi; HUANG Zhi; CHEN Hailing; YAN Dunwei; XUE Zhuzheng

doi:10.19303/j.issn.1008-0384.2023.02.006

Volume 38 Issue 2

Feb. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(2): 166-173

GUO Y Z, HUANG Q, YE X R, et al. Morphological and SRAP Markers-based Genetic Diversity Determination on Phaseolus lunatus L. Germplasms [J]. Fujian Journal of Agricultural Sciences，2023，38（2）：166−173 doi: 10.19303/j.issn.1008-0384.2023.02.006

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GUO Y Z, HUANG Q, YE X R, et al. Morphological and SRAP Markers-based Genetic Diversity Determination on Phaseolus lunatus L. Germplasms [J]. Fujian Journal of Agricultural Sciences，2023，38（2）：166−173 doi: 10.19303/j.issn.1008-0384.2023.02.006

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Morphological and SRAP Markers-based Genetic Diversity Determination on Phaseolus lunatus L. Germplasms

doi: 10.19303/j.issn.1008-0384.2023.02.006

1.
Putian Institute of Agricultural Science, Putian, Fujian　351100, China
2.
Crops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China

Received Date: 2022-09-01
Rev Recd Date: 2022-11-21

Available Online: 2023-03-28

Publish Date: 2023-02-28

Abstract

Abstract

Objective Genetic diversity and relationship of Phaseolus lunatus L. germplasms were determined using the morphological and SRAP markers. Method Twenty-six quantitative and 18 quality traits of 22 lima bean germplasms were analyzed based on the morphological as well as the SRAP molecular markers. Result Twenty-eight pairs of SRAP primers were selected to obtain 158 amplified polymorphic bands with an average polymorphism ratio of 77.75%. The two methods applying different markers clustered the 22 varieties into 3 categories. Genetically, Shanghengshan10-2-6 and Xiahengshan 10-3-3 were relatively close. They might have gone through numerous gene exchanges in the past. Conclusion The 22 lima bean germplasms were rich in genetic diversity. The morphological and SRAP molecular markers-based clustering essentially agreed with the classification by the pod size.
- Phaseolus lunatus L.,
- morphological markers,
- SRAP molecular markers,
- genetic diversity,
- genetic relationship

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References(27)

References

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