Cloning and Expression of <i>VcILR1</i> in Blueberry

HU Lichao; WANG Xiaomin; WANG Xin; WU Yaqiong; WU Wenlong; LYU Lianfei; LI Weilin

doi:10.19303/j.issn.1008-0384.2023.02.004

Volume 38 Issue 2

Feb. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(2): 151-157

HU L C, WANG X M, WANG X, et al. Cloning and Expression of VcILR1 in Blueberry [J]. Fujian Journal of Agricultural Sciences，2023，38（2）：151−157 doi: 10.19303/j.issn.1008-0384.2023.02.004

Citation:

HU L C, WANG X M, WANG X, et al. Cloning and Expression of VcILR1 in Blueberry [J]. Fujian Journal of Agricultural Sciences，2023，38（2）：151−157 doi: 10.19303/j.issn.1008-0384.2023.02.004

HU L C, WANG X M, WANG X, et al. Cloning and Expression of VcILR1 in Blueberry [J]. Fujian Journal of Agricultural Sciences，2023，38（2）：151−157 doi: 10.19303/j.issn.1008-0384.2023.02.004

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Cloning and Expression of VcILR1 in Blueberry

doi: 10.19303/j.issn.1008-0384.2023.02.004

HU Lichao^1
,,
WANG Xiaomin^{2
,
,},
WANG Xin³,
WU Yaqiong²,
WU Wenlong²,
LYU Lianfei²,
LI Weilin^{1
,
,}

1.
Southern Modern Forestry Collaborative Innovation Center/College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu　210037, China
2.
Institute of Botany, Chinese Academy of Sciences in Jiangsu (Nanjing Mem. Sun Yat-Sen Botanical Garden)/ Key Laboratory for Research and Utilization of Plant Resources, Nanjing, Jiangsu　210014, China
3.
Xinghua Forestry Technical Guidance Station, Xnghua, Jiangsu　225700, China

Received Date: 2022-08-04
Rev Recd Date: 2022-10-21

Available Online: 2023-03-28

Publish Date: 2023-02-28

Abstract

Abstract

Objective VcILR1 of blueberry was cloned to analyze the expressions in various plant organs as well as after being treated by gibberellin to decipher its function and association with ovule abortion. Method Using the cDNA of blueberry as the template, VcILR1 was cloned by RT-PCR. Bioinformatics on the amino acid sequence was performed utilizing ProtParam and other relevant tools. Expressions of the gene in the flowers, leaves, stems, fruits, and roots of the plant as well as those in green and ripe fruits after being treated by gibberellin were determined. Result VcILR1 was successfully cloned and found to contain 976 bp ORF encoding 325 amino acids with two conserved structural domains, peptidase_M20 and M20_dimer, a molecular mass of 35364.20 kDa, a theoretical isoelectric point of 5.67, an instability coefficient of 43.57, and a lipid coefficient of 88.55. It was an unstable protein with a hydrophilic mean value of −0.054. A phylogenetic tree analysis showed the gene to closely relate to Camellia sinensis IAA-Leucine Resistant 1-like (ILL) gene. The qRT-PCR analysis revealed that the gene was expressed tissue-specific with the highest in the leaves, and that the gibberellin treatment up-regulated the expression in the fruits. Conclusion The expressions of VcILR1 in the blueberries significantly increased after being treated by gibberellin. It suggested that the plant hormone could stimulate IAA generation in the fruits and might also affect the ovule development.
- Blueberry,
- VcILR1 gene,
- gene cloning,
- seedless

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References(26)

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