Cellulose Enzymatic Activity of <i>Rhizopus oryzae </i>and Degrading Efficiency on Agricultural Waste

WANG Weiying; KE Yexin; LI Honghao; XIAO Shun; WANG Zonghua

doi:10.19303/j.issn.1008-0384.2023.01.010

Volume 38 Issue 1

Jan. 2023

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WANG W Y, KE Y X, LI H H, et al. Cellulose Enzymatic Activity of Rhizopus oryzae and Degrading Efficiency on Agricultural Waste [J]. Fujian Journal of Agricultural Sciences，2023，38（1）：75−80 doi: 10.19303/j.issn.1008-0384.2023.01.010

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WANG W Y, KE Y X, LI H H, et al. Cellulose Enzymatic Activity of Rhizopus oryzae and Degrading Efficiency on Agricultural Waste [J]. Fujian Journal of Agricultural Sciences，2023，38（1）：75−80 doi: 10.19303/j.issn.1008-0384.2023.01.010

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Cellulose Enzymatic Activity of Rhizopus oryzae and Degrading Efficiency on Agricultural Waste

doi: 10.19303/j.issn.1008-0384.2023.01.010

WANG Weiying^{1, 2
,},
KE Yexin²,
LI Honghao²,
XIAO Shun^{2
,
,},
WANG Zonghua²

1.
Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian　363005, China
2.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian　360001, China

Received Date: 2022-05-25
Rev Recd Date: 2022-07-31

Available Online: 2023-03-28

Publish Date: 2023-01-28

Abstract

Abstract

Objective Activity and efficiency of agriculture waste-degrading capability of Rhizopus oryzae C1 were investigated. Method R. oryzae C1 isolated from mold-infected corn cobs was cultured in a liquid medium to measure the activities of carboxymethyl cellulase (CMCase), filter paper enzyme (FPase), and microcrystalline cellulase (Avicelase). Subsequently, the fermentation broth was introduced onto vegetable leaves to estimate the decomposition effect of R. oryzae C1. Result The greatest enzymatic activity of CMCase was detected in R. oryzae C1 at 35.33 U·mL⁻¹ followed by those of Avicelase and FPase. The isolated R. oryzae C1 displayed a significant degradation effect on the vegetable farm waste.Within 6 d after introduction of the fermentation broth on vegetable leaves, 47% of the starting waste material was liquified. In contrast, no decomposition appeared on control. Conclusion R. oryzae C1 had shown to be a fiber-degrading fungus with a high CMCase activity and a significant decomposition efficiency on farm discards. The bacterial fermentation broth demonstrated a rapid and high rate in decomposing vegetable leaves. It was conceivably that R. oryzae C1 or its derivatives be developed to degrade field waste materials and vastly curtail pollution and improve ecology of a farming environment.
- Rhizopus oryzae C1,
- agriculture waste,
- cellulose,
- enzyme activity,
- fermentation

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References(27)

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