Relationship between InDel Polymorphism of FN1 Gene and Growth Traits of Goats

  Objective   Detection of fibronectin 1 (FN1) gene in DNA pool and association of the gene InDel polymorphism with bone differentiation, formation, and cell migration of goats were studied to locate the molecular marker and to confirm target for breeding and trait improvement.

  Method   Genetic polymorphisms of the FN1 gene in Fuqing, Nubian, and Jianyang Daer goats were detected in the DNA pool with PCR-RFLP. Relationship between the genotypes and meat production traits of the goats were analyzed.

  Result  Among the 7 predicted sites in all 3 goat species, only Del66652 locus exhibited a polymorphism belonging to the II and ID genotypes with a frequency not in HWE (P＞0.05) and less than 0.25 on the polymorphism information content. The cannon circumference of the ID genotype was significantly greater than that of the II genotype (P＜0.05), while the chest circumference index of the ID genotype significantly greater than that of the II genotype (P＜0.01) in the Nubian goats. In the Jianyang Daer goats, the chest circumference and the cannon circumference indices of the ID genotype were significantly greater than those of the II genotype (P＜0.05).

  Conclusion  Del66652 locus significantly correlated with the growth traits of Nubian and Jianyang Daer goats with the ID being the dominant genotype. The locus closely associated with the growth traits of the goats and was considered a candidate molecular marker to be use in breeding programs.