Nutrients and Microbial Community in Recirculating Aquaculture System for <i>Plectropomus leopardus </i>Aquaculture

LIU Xin; KANG Jianping; LIN Gang; MIAO Fan; YE Hui; RAO Xiaozhen; ZHONG Chuanming

doi:10.19303/j.issn.1008-0384.2022.06.010

Volume 37 Issue 6

Jun. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(6): 754-764

LIU X, KANG J P, LIN G, et al. Nutrients and Microbial Community in Recirculating Aquaculture System for Plectropomus leopardus Aquaculture [J]. Fujian Journal of Agricultural Sciences，2022，37（6）：754−764 doi: 10.19303/j.issn.1008-0384.2022.06.010

Citation:

LIU X, KANG J P, LIN G, et al. Nutrients and Microbial Community in Recirculating Aquaculture System for Plectropomus leopardus Aquaculture [J]. Fujian Journal of Agricultural Sciences，2022，37（6）：754−764 doi: 10.19303/j.issn.1008-0384.2022.06.010

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Nutrients and Microbial Community in Recirculating Aquaculture System for Plectropomus leopardus Aquaculture

doi: 10.19303/j.issn.1008-0384.2022.06.010

LIU Xin^1
,,
KANG Jianping²,
LIN Gang^{1, 3
,
,},
MIAO Fan¹,
YE Hui²,
RAO Xiaozhen^{1, 3},
ZHONG Chuanming²

1.
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian　350117, China
2.
Fujian Fishery Technology Extension Station, Fuzhou, Fujian　350001, China
3.
Fujian Key Laboratory of Sustainable Utilization of Special Marine Life Resources, Fuzhou, Fujian　350117, China

Received Date: 2022-01-11
Rev Recd Date: 2022-04-21

Available Online: 2022-08-07

Publish Date: 2022-06-28

Abstract

Abstract

Objective Nutrients content and microbial community in a recirculating aquaculture system (RAS) for Plectropomus leopardus aquaculture were analyzed to provide theoretical and practical reference for the aquaculture operation. Method Indicators for the water quality of the influent, fish ponds, effluent, biological filter, and ultraviolet disinfection tank as well as the Vibrio count and microbial community at various stages of the RAS were determined based upon the high-throughput sequencing results. Result The concentrations of NH₄⁺-N, NO₃⁻-N and PO₄³⁻-P in the water increased at the stages where fish were present and declined where disinfection took place. It was the opposite on NO₂⁻-N. Vibrio count was fewer in the exogenous seawater than in any downstream points of RAS which rose significantly after flowing through the fish ponds and diminished after the biofiltration treatment. At phylum level, the dominant microbes in the system were Proteobacteria, Bacteroidota, and Firmicutes. At genus level, there are mainly Pseudoalteromonas, Photobacterium, Vibrio, and Lactobacillus. The main Vibrio species was V. ponticus, the ration of which was 66.17%. Conclusion The RAS system was effective in reducing the $ {\rm{NH}}_4^+ $-N, NO₃⁻-N, ${\rm{PO}}_4^{3-} $-P, and vibrio population in the water. The composition of the microbial community in the circulating water was unveiled.
- Plectropomus leopardus,
- recirculating aquaculture system,
- high-throughput sequencing,
- microbial community structure,
- vibrio

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References(39)

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