Genetic Evolution of <i>F1L, B2L,</i> and<i> GIF</i> of Orf Virus in Four Clinical Samples

LIN Yusheng; LIU Weiwei; ZHANG Long; JIANG Jinxiu; ZHANG Jingpeng; LIU Qinghua; HU Qilin

doi:10.19303/j.issn.1008-0384.2022.06.009

Volume 37 Issue 6

Jun. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(6): 748-753

LIN Y S, LIU W W, ZHANG L, et al. Genetic Evolution of F1L, B2L, and GIF of Orf Virus in Four Clinical Samples [J]. Fujian Journal of Agricultural Sciences，2022，37（6）：748−753 doi: 10.19303/j.issn.1008-0384.2022.06.009

Citation:

LIN Y S, LIU W W, ZHANG L, et al. Genetic Evolution of F1L, B2L, and GIF of Orf Virus in Four Clinical Samples [J]. Fujian Journal of Agricultural Sciences，2022，37（6）：748−753 doi: 10.19303/j.issn.1008-0384.2022.06.009

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Genetic Evolution of F1L, B2L, and GIF of Orf Virus in Four Clinical Samples

doi: 10.19303/j.issn.1008-0384.2022.06.009

LIN Yusheng^1
,,
LIU Weiwei^{1, 2
,},
ZHANG Long^{1, 2},
JIANG Jinxiu¹,
ZHANG Jingpeng¹,
LIU Qinghua²,
HU Qilin^{1
,
,}

1.
Institute of Animal Husbandry & Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2022-03-31
Accepted Date: 2022-03-31
Rev Recd Date: 2022-04-14

Available Online: 2022-06-20

Publish Date: 2022-06-28

Abstract

Abstract

Objective Molecular epidemiology of the Orf virus (ORFV) in the diseased sheep and goats from farms in Fujian Province in 2021 were studied. Method The ORFV genes, F1L, B2L, and GIF, from 4 clinical samples of the diseased animals were confirmed positive by PCR prior to cloning for sequence determination. The data were uploaded to GenBank for a comparative analysis using bioinformatics software. Result The nucleotide sequence similarities were 98.0%–98.8% on F1L , 98.4%–99.5% on B2L, and 97.2%–99.6% on GIF; those related to the domestic epidemic strains, 97.4%–99.4% on F1L, 97.4%–99.9% on B2L, and 95.7%–99.0% on G1F; those related to the foreign epidemic strains, 97.5%–98.6% on F1L, 97.9%–98.9% on B2L, and 95.6%–98.0% on G1F; those related to the German D1701 attenuated strain, 96.5%–96.8% on F1L, 98.0%–98.7% on B2L, and 96.0%–96.4% on G1F; and those related to the NZ2 reference strain, 97.7%–98.1% on F1L, 97.5%–98.1% on B2L, and 95.9%–96.1% on G1F. The phylogenetic tree showed the ORFVs closely related to the strains from Jilin (NA17, SY17, and CL18), Fujian (SJ1), and India (MP), but remotely with OV-IA82 from the USA, D1701 attenuated strain from German, NZ2 reference strain from New Zealand, and the vaccine strain from China. It appeared that the current epidemic strain might be a recombination of Fujian, Jilin GZ18, and Indian MP strains. Conclusion The sequences of B2L, F1L, and GIF from the 2021 clinical specimens were uploaded to GenBank database enriching its collection. This study characterized the ORFVs found in the sheep and goats infected by the epidemic and facilitated further studies in the disease control and prevention.
- Orf virus,
- F1L,
- B2L,
- GIF,
- evolution analysis

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References(17)

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