Effect of Spraying Methyl Jasmonate on Oxidase Activity and Interleaf Microbial Diversity of Tobacco Leaves

WU Jiaxiang; LIU Tao; XIE Yonghui; HUANG Zhiyuan; ZI Shuhui

doi:10.19303/j.issn.1008-0384.2022.06.005

Volume 37 Issue 6

Jun. 2022

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WU J X, LIU T, XIE Y H, et al. Effect of Spraying Methyl Jasmonate on Oxidase Activity and Interleaf Microbial Diversity of Tobacco Leaves [J]. Fujian Journal of Agricultural Sciences，2022，37（6）：720−726 doi: 10.19303/j.issn.1008-0384.2022.06.005

Citation:

WU J X, LIU T, XIE Y H, et al. Effect of Spraying Methyl Jasmonate on Oxidase Activity and Interleaf Microbial Diversity of Tobacco Leaves [J]. Fujian Journal of Agricultural Sciences，2022，37（6）：720−726 doi: 10.19303/j.issn.1008-0384.2022.06.005

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Effect of Spraying Methyl Jasmonate on Oxidase Activity and Interleaf Microbial Diversity of Tobacco Leaves

doi: 10.19303/j.issn.1008-0384.2022.06.005

WU Jiaxiang^{1, 2
,},
LIU Tao^{1, 2},
XIE Yonghui³,
HUANG Zhiyuan^{1, 2},
ZI Shuhui^{1, 2
,
,}

1.
National-Local Joint Engineering Research Center on Germplasms Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, Yunnan　650201, China
2.
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan　650201, China
3.
Kunming Tobacco Company of Yunnan Province, Kunming, Yunnan　650051, China

Received Date: 2022-02-23
Rev Recd Date: 2022-06-01
Publish Date: 2022-06-28

Abstract

Abstract

Objective Effects of spraying methyl jasmonate (MeJA) on the antioxidant enzyme activity and interleaf microbial diversity of tobacco leaves were investigated. Method Superoxide dismutase (SOD) activity, peroxidase (POD) activity, total phenolic content, hydrogen peroxide (H₂O₂) accumulation as well as abundance and diversity of interleaf bacteria and fungi in tobacco leaves were measured 72 h after spraying the plant growth regulator at 0.5 mmol·L^-1 (+MeJA) or without (CK) as control. Result The MeJA spray significantly increased SOD and POD activities and H₂O₂ accumulation but reduced the microbial abundance and increased the fungal diversity in the tobacco leaves. The SOD activity and H₂O₂ accumulation significantly inversely correlated with Golovinomyces, whereas POD positively correlated with Ralstonia and Burkholderia-Caballeronia-Paraburkholderia. Conclusion Tobacco leaves sprayed by MeJA not only activated the antioxidant enzyme activity but also altered the dominant fungi distribution in them, resulting in a correlation between oxidase activity and fungal genus share in tobacco leaves.
- methyl jasmonate,
- tobacco,
- phyllosphere microorganisms,
- antioxidant enzyme

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References(22)

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