Effect of TOR Inhibitor Rapamycin on Growth, Development, and Autophagy of <i>Peronophythora litchii</i>

LV Lin; YANG Chengdong; ZHANG Xue; YU Ge; CHEN Taixu; CHEN Qinghe

doi:10.19303/j.issn.1008-0384.2022.011.010

Volume 37 Issue 11

Nov. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(11): 1448-1453

LV L, YANG C D, ZHANG X, et al. Effect of TOR Inhibitor Rapamycin on Growth, Development, and Autophagy of Peronophythora litchii [J]. Fujian Journal of Agricultural Sciences，2022，37（11）：1448−1453 doi: 10.19303/j.issn.1008-0384.2022.011.010

Citation:

LV L, YANG C D, ZHANG X, et al. Effect of TOR Inhibitor Rapamycin on Growth, Development, and Autophagy of Peronophythora litchii [J]. Fujian Journal of Agricultural Sciences，2022，37（11）：1448−1453 doi: 10.19303/j.issn.1008-0384.2022.011.010

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Effect of TOR Inhibitor Rapamycin on Growth, Development, and Autophagy of Peronophythora litchii

doi: 10.19303/j.issn.1008-0384.2022.011.010

LV Lin^{1, 2
,},
YANG Chengdong^{1, 2},
ZHANG Xue¹,
YU Ge¹,
CHEN Taixu¹,
CHEN Qinghe^{1, 2
,
,}

1.
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education/College of Plant Protection, Hainan University, Haikou, Hainan　570228, China
2.
Sanya Nanfan Research Institute, Hainan University/Hainan Yazhou Bay Seed Laboratory, Sanya, Hainan　572025, China

Received Date: 2022-08-25
Rev Recd Date: 2022-09-12

Available Online: 2022-11-29

Publish Date: 2022-11-28

Abstract

Abstract

Objective Effects of the target of rapamycin (TOR) inhibitor rapamycin on the growth, development, pathogenicity, and autophagy of Peronophythora litchi were studied. Method The mycelial growth, sporangial production, zoospore release, and pathogenicity of the P. litchi after being treated by the TOR inhibitor rapanmycin in varying concentrations were determined, while the mycelial autophagy observed under a microscope with MDC staining. Result Rapamycin exhibited significant inhibitory effect on the mycelial growth of P. litchii with an EC₅₀ value of 29.18 ng·mL⁻¹ and varying extents of increases with higher dosages. When treated with 25.0 ng·mL⁻¹ rapamycin, the inhibition rate was 45.3% and the sporulation at 8.0×10⁴ sporangia·mL⁻¹ (which was 27.9% of control), while the zoospore release increased, but the virulence of P. litchii significantly reduced. The MDC-stained autophagosomes significantly increased as well. Conclusion The TOR signaling pathway inhibitor rapamycin played an important role in the mycelial growth, development, and virulence of P. litchii.
- Peronophythora litchii,
- rapamycin,
- TOR inhibitor,
- growth and development,
- autophagy

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References(24)

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