Genome-wide Identification and Expressions of Banana GDSL Lipase Gene Family

ZHANG Chengyu; REN Wenhui; GENG Xiaohui; LI Dan; WU Hao; NI Shanshan; WANG Mengge; LUO Binbin; XU Han; LAI Zhongxiong

doi:10.19303/j.issn.1008-0384.2022.011.007

Volume 37 Issue 11

Nov. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(11): 1415-1429

ZHANG C Y, REN W H, GENG X H, et al. Genome-wide Identification and Expressions of Banana GDSL Lipase Gene Family [J]. Fujian Journal of Agricultural Sciences，2022，37（11）：1415−1429 doi: 10.19303/j.issn.1008-0384.2022.011.007

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ZHANG C Y, REN W H, GENG X H, et al. Genome-wide Identification and Expressions of Banana GDSL Lipase Gene Family [J]. Fujian Journal of Agricultural Sciences，2022，37（11）：1415−1429 doi: 10.19303/j.issn.1008-0384.2022.011.007

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Genome-wide Identification and Expressions of Banana GDSL Lipase Gene Family

doi: 10.19303/j.issn.1008-0384.2022.011.007

1.
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Institute de la Recherche Interdisciplinaire de Toulouse, IRIT-ARI, Toulouse　31300, France

Received Date: 2022-03-24
Rev Recd Date: 2022-06-14

Available Online: 2022-12-28

Publish Date: 2022-11-28

Abstract

Abstract

Objective Sequences and functions of banana GDSL lipase gene family (MaGDSL) were studied. Methods Bioinformatics was applied to identify the genomes, analyze the distributions of chromosomes, promoter cis-acting elements, and transcription factor binding sites (TFBS), and determine the physicochemical properties, gene structure, conserved motifs, and phylogenetic relationships of the encoded proteins of the MaGDSL family. Based on the transcriptome database, the expressions of MaGDSLs in the high (45 ℃)- or low temperature (4 ℃)-treated leaves, FocTR4-infested roots, and the natural or ethylene ripened fruits were measured. And qRT-PCR was employed to obtain the expressions of MaGDSL members in pollen. Results The banana A genome had 76 MaGDSLs of 9 subfamilies distributed on 11 chromosomes. The coding region of each member was 1014–2193 bp. Five of the members contained varied number of transcripts, but most of them had 5 exons and 4 introns. The encoded proteins had signal peptides localized mainly in the inner and outer membranes. The MaGDSLs had 3 tandem repeat clusters on chromosomes 4 and 8, 6 tandem repeat pairs on chromosomes 1, 6, 7, 8, 9, and 10, and 22 fragment repeat pairs on all except chromosome 11. The expressions of MaGDSLs in banana leaves and roots were highly variable with individual members regulated by high and low temperature stresses as well as the blast fungus. Five members (i.e., MaGDSL4-5, 8-1, 8-12, 9-4, 9-5) were repressed, but MaGDSL2-1 and MaGDSL6-8 induced, by high- or low-temperature exposure, while MaGDSL4-1 and MaGDSL11-1 regulated by low temperature and FocTR4, MaGDSL5–8 responded only to low temperature, MaGDSL2-2 and MaGLP10-5 sensitive to various treatments, and MaGDSL1-1 highly expressed in the roots and pollens. Conclusion MaGDSLs might play an important role in the growth and development of banana plants. Some of the members responded specifically to certain biotic and/or abiotic stresses.
- Banana,
- GDSL lipase gene,
- genome,
- stress by adversity,
- expression analysis

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