Detection of Quinolone Resistance Genes and <i>QRDR</i> Mutation in <i>Riemerella anatipestifer </i>Found in Putian, Fujian

LIN Binbin; LIN Zhimin; XIE Bilin; WENG Handong; WANG Xiuzhen; CHENG Longfei; FU Guanghua; LIU Rongchang

doi:10.19303/j.issn.1008-0384.2022.011.005

Volume 37 Issue 11

Nov. 2022

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(11): 1400-1406

LIN B B, LIN Z M, XIE B L, et al. Detection of Quinolone Resistance Genes and QRDR Mutation in Riemerella anatipestifer Found in Putian, Fujian [J]. Fujian Journal of Agricultural Sciences，2022，37（11）：1400−1406 doi: 10.19303/j.issn.1008-0384.2022.011.005

Citation:

LIN B B, LIN Z M, XIE B L, et al. Detection of Quinolone Resistance Genes and QRDR Mutation in Riemerella anatipestifer Found in Putian, Fujian [J]. Fujian Journal of Agricultural Sciences，2022，37（11）：1400−1406 doi: 10.19303/j.issn.1008-0384.2022.011.005

Citation:

PDF( 591 KB)

Detection of Quinolone Resistance Genes and QRDR Mutation in Riemerella anatipestifer Found in Putian, Fujian

doi: 10.19303/j.issn.1008-0384.2022.011.005

1.
Putian Institute of Agricultural Sciences, Putian, Fujian　351144, China
2.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China

Received Date: 2022-06-29
Rev Recd Date: 2022-10-17

Available Online: 2022-12-28

Publish Date: 2022-11-28

Abstract

Abstract

Objective Epidemiology and mechanism of quinolone-related drug resistance genes of Riemerella anatipestifer found in ducks in Putian, Fujian were investigated. Method The K-B method was applied to examine 55 strains of R. anatipestifer to determine their resistance to 6 types of quinolones. PCR was employed to detect and analyze the quinolone resistance determining region (QRDR) and plasmid mediated quinolone resistance (PMQR) genes. Result More than half of the 55 strains examined were multi-resistant and highly resistant to pipemidic acid, norfloxacin, enrofloxacin, and ciprofloxacin; and more than 90% of them highly sensitive to ofloxacin. PCR did not find the 4 PMQRs, i.e., qnrA, qnrB, qnrS, and oqxA, in the specimens. On the other hand, 6 QRDR mutations in the deduced amino acid sequences of gyrA, including S83R and S83I, S84P, D87A and D87G, A183V, N202E, and Y211H were detected. Among them, S83I, N202E, and Y211H had the highest mutation rates of 98.18% (54/55), 92.73% (51/55), and 90.91% (50/55), respectively. Of which, the S83I (38/39) and S83R (1/39) mutations were found in gyrA of 39 enrofloxacin-resistant strains, and the S83I mutations also in that of 3 enrofloxacin-sensitive strains. There was only one enrofloxacin-intermediated strain that had the F155S mutation in parC, which was not previously reported to exist in R. anatipestifer. Conclusion The quinolone-resistance of R. anatipestifer found in ducks in Putian was severe. No PMQR was detected in the samples, but the S83I mutation in gyrA of QRDR could be the major culprit that involved in the drug resistance of R. anatipestifer.
- Riemerella anatipestifer,
- quinolones,
- drug resistance gene,
- gyrA gene,
- parC gene

FullText(HTML)

References(27)

References

[1]	杨永胜, 陈研, 薛亚飞, 等. 4株鹅源鸭疫里默氏杆菌生物学特性分析 [J]. 中国动物传染病学报, 2020, 28(5):62−66. YANG Y S, CHEN Y, XUE Y F, et al. Biological characteristics of four Riemerella anatipestifer isolates from geese [J]. Chinese Journal of Animal Infectious Diseases, 2020, 28(5): 62−66.(in Chinese)
[2]	胡洋, 黄书伦, 程方俊, 等. 鹅源鸭疫里默氏杆菌的分离鉴定及药敏试验 [J]. 黑龙江畜牧兽医, 2018(2):112−115,244. HU Y, HUANG S L, CHENG F J, et al. Isolation, identification and drug sensitivity test of Riemerella anatipestifer from goose and duck [J]. Heilongjiang Animal Science and Veterinary Medicine, 2018(2): 112−115,244.(in Chinese)
[3]	LOZICA L, MAZIĆ M, GOTTSTEIN Ž. A case study of a Riemerella anatipestifer infection on a commercial turkey farm in Croatia [J]. European Poultry Science, 2021, 85: 1−7.
[4]	吴彩艳, 廖申权, 戚南山, 等. 广东省鸭疫里默氏杆菌流行病学监测及遗传进化关系 [J]. 华南农业大学学报, 2022, 43(2):1−10. WU C Y, LIAO S Q, QI N S, et al. Epidemiological surveillance and genetic evolution of Riemerella anatipestifer in Guangdong Province [J]. Journal of South China Agricultural University, 2022, 43(2): 1−10.(in Chinese)
[5]	朱元军, 王小莺, 杨德鸿, 等. 我国南方部分地区鸭疫里氏杆菌的分离鉴定及耐药情况调查 [J]. 畜牧与兽医, 2019, 51(12):106−111. ZHU Y J, WANG X Y, YANG D H, et al. Identification and antibiotic resistance analysis of Riemerella anatipestifer isolated from diseased ducks in South China [J]. Animal Husbandry & Veterinary Medicine, 2019, 51(12): 106−111.(in Chinese)
[6]	金龙翔, 程龙飞, 陈红梅, 等. 鸭疫里默氏菌对氟喹诺酮药物的主动外排作用 [J]. 福建农业学报, 2014, 29(5):413−416. JIN L X, CHENG L F, CHEN H M, et al. The active efflux of Riemerella anatipestifer for fluoroquinolone antibiotics [J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 413−416.(in Chinese)
[7]	邵莉萍, 张继瑜. 喹诺酮类药物的抗菌活性与细菌耐药性研究进展 [J]. 中国畜牧兽医, 2017, 44(9):2773−2782. SHAO L P, ZHANG J Y. Research advances on antibacterial activity and bacterial resistance of quinolones [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(9): 2773−2782.(in Chinese)
[8]	LI Y F, JIANG H X, XIANG R, et al. Effects of two efflux pump inhibitors on the drug susceptibility of Riemerella anatipestifer isolates from China [J]. Journal of Integrative Agriculture, 2016, 15(4): 929−933. doi: 10.1016/S2095-3119(15)61031-0
[9]	王美珍, 陈昌福, 刘振兴, 等. 嗜水气单胞菌对四环素类和氟喹诺酮类药物的耐药性研究 [J]. 华中农业大学学报, 2011, 30(1):89−93. WANG M Z, CHEN C F, LIU Z X, et al. In vitro study on drug-resistance characteristics of Aeromonas hydrophila to tetracyclines and fluoroquinolones [J]. Journal of Huazhong Agricultural University, 2011, 30(1): 89−93.(in Chinese)
[10]	马叶本. 我国部分地区科瓦利斯沙门菌分离鉴定、耐药性及PFGE分子分型研究[D]. 广州: 华南农业大学, 2019. MA Y B. Isolation, identification, drug resistance and PFGE molecular typing of Salmonella corvallis in some areas of China[D]. Guangzhou: South China Agricultural University, 2019. (in Chinese)
[11]	姜芹, 孙冰清, 顾欣, 等. 动物源大肠埃希菌gyrA基因突变与氟喹诺酮耐药相关性分析 [J]. 动物医学进展, 2020, 41(4):63−66. JIANG Q, SUN B Q, GU X, et al. Analysis of mutations in gyrA gene associated with fluoroquinolone resistance in Escherichia coli isolates from animals [J]. Progress in Veterinary Medicine, 2020, 41(4): 63−66.(in Chinese)
[12]	陈杨, 刘理慧, 吴翠蓉, 等. 喹诺酮类耐药基因qnr的研究进展 [J]. 国外医药(抗生素分册), 2021, 42(4):193−203. CHEN Y, LIU L H, WU C R, et al. Progress in the mechanism of quinolone resistance of qnr family [J]. World Notes on Antibiotics, 2021, 42(4): 193−203.(in Chinese)
[13]	GUERRA B, HELMUTH R, THOMAS K, et al. Plasmid-mediated quinolone resistance determinants in Salmonella spp. isolates from reptiles in Germany [J]. Journal of Antimicrobial Chemotherapy, 2010, 65(9): 2043−2045. doi: 10.1093/jac/dkq242
[14]	GAY K, ROBICSEK A, STRAHILEVITZ J, et al. Plasmid-mediated quinolone resistance in non-typhi serotypes of Salmonella enterica [J]. Clinical Infectious Diseases, 2006, 43(3): 297−304. doi: 10.1086/505397
[15]	HANSEN L H, SØRENSEN S J, JØRGENSEN H S, et al. The prevalence of the OqxAB multidrug efflux pump amongst olaquindox-resistant Escherichia coli in pigs [J]. Microbial Drug Resistance (Larchmont, N Y), 2005, 11(4): 378−382. doi: 10.1089/mdr.2005.11.378
[16]	张亚楠. 广东省鸭疫里氏杆菌耐药性调查及floR传播分子特征研究[D]. 广州: 华南农业大学, 2016. ZHANG Y N. Monitoring of antibiotic resistance and dissemination of FloR among Riemerella anatipestifer isolated from ducks and geese in guangdong[D]. Guangzhou: South China Agricultural University, 2016. (in Chinese)
[17]	郭晶晶, 侯思梦, 刘连杰, 等. 猪源大肠埃希菌中质粒介导喹诺酮耐药(PMQR)基因的多样性研究 [J]. 西北农业学报, 2021, 30(11):1611−1617. doi: 10.7606/j.issn.1004-1389.2021.11.003 GUO J J, HOU S M, LIU L J, et al. Diversity of plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli form swine [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2021, 30(11): 1611−1617.(in Chinese) doi: 10.7606/j.issn.1004-1389.2021.11.003
[18]	CHEN Q W, GONG X W, ZHENG F Y, et al. Interplay between the phenotype and genotype, and efflux pumps in drug-resistant strains of Riemerella anatipestifer [J]. Frontiers in Microbiology, 2018, 9: 2136. doi: 10.3389/fmicb.2018.02136
[19]	杨芳芳. 鸭疫里默氏杆菌氨基糖苷类和喹诺酮类耐药基因的检测与序列分析[D]. 泰安: 山东农业大学, 2013. YANG F F. Detection and sequence analysis of aminoglycoside and quinolones resistance genes in Riemerella anatipestfer[D]. Taian: Shandong Agricultural University, 2013. (in Chinese)
[20]	王婧, 刁小龙, 陈晓兰, 等. 猪源大肠埃希菌喹诺酮类耐药基因的检测及分析 [J]. 甘肃农业大学学报, 2020, 55(2):9−15. WANG J, DIAO X L, CHEN X L, et al. Detection and analysis of resistance genes in quinolones-resistant Escherichia coli isolated from swine [J]. Journal of Gansu Agricultural University, 2020, 55(2): 9−15.(in Chinese)
[21]	郑来煜, 刘敏, 王占黎, 等. 氟喹诺酮耐药肠球菌gyrA和parC基因突变特征及其与环丙沙星耐药相关性分析 [J]. 现代预防医学, 2020, 47(14):2672−2676. ZHENG L Y, LIU M, WANG Z L, et al. Mutations and characterization in gyrA and parC genes among fluoroquinolone-resistant Enterococcus associated with ciprofloxacin resistance [J]. Modern Preventive Medicine, 2020, 47(14): 2672−2676.(in Chinese)
[22]	张友春, 王志平. 肺炎克雷伯菌gyrA和parC基因突变与喹诺酮耐药相关性研究 [J]. 浙江医学教育, 2013, 12(3):48−50. doi: 10.3969/j.issn.1672-0024.2013.03.018 ZHANG Y C, WANG Z P. The relationship between gyrA and parC gene mutations of Klebsiella pneumoniae and quinolone resistance [J]. Zhejiang Medical Education, 2013, 12(3): 48−50.(in Chinese) doi: 10.3969/j.issn.1672-0024.2013.03.018
[23]	SUN N, LIU J H, YANG F, et al. Molecular characterization of the antimicrobial resistance of Riemerella anatipestifer isolated from ducks [J]. Veterinary Microbiology, 2012, 158(3/4): 376−383.
[24]	金龙翔. 鸭疫里默氏菌对氟喹诺酮类药物耐药机制的研究[D]. 南昌: 江西农业大学, 2014. JIN L X. The mechanism of quinolone resistance in Riemerella anatipestifer[D]. Nanchang: Jiangxi Agricultural University, 2014. (in Chinese)
[25]	孙亚妮. GN52株鸭疫里默氏杆菌的传代培养和鸭疫里默氏杆菌分离株耐药性的研究[D]. 泰安: 山东农业大学, 2008. SUN Y N. The serial subcultivation of GN52 and the research of drug resistance of Riemerella anatipestifer isolates[D]. Taian: Shandong Agricultural University, 2008. (in Chinese)
[26]	刘颖, 苏敬良, 刘文华. 鸭疫里默氏菌的gyrA基因的检测与变异位点的分析 [J]. 中国兽医杂志, 2007, 43(1):12−14. doi: 10.3969/j.issn.0529-6005.2007.01.004 LIU Y, SU J L, LIU W H. Detection of gyrA gene of Riemerella anatipestifer and analysis of mutation sites [J]. Chinese Journal of Veterinary Medicine, 2007, 43(1): 12−14.(in Chinese) doi: 10.3969/j.issn.0529-6005.2007.01.004
[27]	ZHU D K, ZHENG M Y, XU J G, et al. Prevalence of fluoroquinolone resistance and mutations in the gyrA, parC and parE genes of Riemerella anatipestifer isolated from ducks in China [J]. BMC Microbiology, 2019, 19(1): 271. doi: 10.1186/s12866-019-1659-4