Isolation, Identification, and <i>S1</i> Gene Sequencing of Two Strains of Avian Infectious Bronchitis Virus

LIN Yusheng; LIU Weiwei; ZHANG Long; JIANG Jinxiu; ZHANG Jingpeng; LIU Qinghua; HU Qilin

doi:10.19303/j.issn.1008-0384.2022.011.003

Volume 37 Issue 11

Nov. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(11): 1388-1393

LIN Y S, LIU W W, ZHANG L, et al. Isolation, Identification, and S1 Gene Sequencing of Two Strains of Avian Infectious Bronchitis Virus [J]. Fujian Journal of Agricultural Sciences，2022，37（11）：1388−1393 doi: 10.19303/j.issn.1008-0384.2022.011.003

Citation:

LIN Y S, LIU W W, ZHANG L, et al. Isolation, Identification, and S1 Gene Sequencing of Two Strains of Avian Infectious Bronchitis Virus [J]. Fujian Journal of Agricultural Sciences，2022，37（11）：1388−1393 doi: 10.19303/j.issn.1008-0384.2022.011.003

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Isolation, Identification, and S1 Gene Sequencing of Two Strains of Avian Infectious Bronchitis Virus

doi: 10.19303/j.issn.1008-0384.2022.011.003

LIN Yusheng^1
,,
LIU Weiwei^{1, 2
,},
ZHANG Long^{1, 2},
JIANG Jinxiu¹,
ZHANG Jingpeng¹,
LIU Qinghua²,
HU Qilin^{1
,
,}

1.
Institute of Animal Husbandry & Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2022-02-18
Rev Recd Date: 2022-09-02

Available Online: 2022-12-28

Publish Date: 2022-11-28

Abstract

Abstract

Objective Epidemiology and genetic variations of the infectious bronchitis virus (IBV) in Fujian province were studied. Method Two strains of virus isolated from the diseased chickens in Fujian in 2021 were identified by chicken embryo pathogenicity test, electron microscope observation, and RT-PCR. S1 genes of the isolates were cloned, sequenced, and analyzed using biological software. Result The two IBV strains were code named FJ-NP01 and FJ-FZ01. The full length of S1 of FJ-NP01 was 1629 nt encoding 543 amino acids, and that of FJ-FZ01, 1620 nt encoding 540 amino acids. The S1 gene cleavage site of FJ-FZ01 was HRRRR, same as all reference strains of genotype I branch; while that of FJ-NP01 HRRKR differed from the reported site of IBV isolated from genotype Ⅳ but same as that of TC07-2 reference strain of genotype Ⅵ. The homology of nucleotide and amino acid between the two isolates was 83.2% and 79.6%, respectively, but merely 75.7%–76.3% and 77.1%–83.5% with the Mass-type conventional vaccines H120 and H52, respectively. Further analysis showed that FJ-NP01 was from a recombination event between CK CH GD LZ12-4 and L-1148, the homology of nucleotide acid between 1438–1506 nt of FJ-NP01 with CK CH GD LZ12-4 was 97%, and 95.9% between the other nucleotide acid of S1 gene with L-1148 . Conclusion It appeared that the IBV epidemic experienced in the province was complex in nature and that the existing Mass vaccines would not provide sufficient immune protection to deter the spread.
- Infectious bronchitis virus,
- isolation,
- identification,
- S1 genes,
- sequence analysis

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References(20)

References

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