TrnL-F and ISSR Primer-based Molecular Identification and Genetic Diversity Determination on <i>Camellia impressinervis</i> and Three Closely Related Species

CHEN Ying; ZENG Jingjing; LV Zhilong; QIU Mingyue; GUO Beilin; YAO Limin; YAN Shujun

doi:10.19303/j.issn.1008-0384.2022.010.008

Volume 37 Issue 10

Oct. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(10): 1298-1304

CHEN Y, ZENG J J, LV Z L, et al. TrnL-F and ISSR Primer-based Molecular Identification and Genetic Diversity Determination on Camellia impressinervis and Three Closely Related Species [J]. Fujian Journal of Agricultural Sciences，2022，37（10）：1298−1304 doi: 10.19303/j.issn.1008-0384.2022.010.008

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CHEN Y, ZENG J J, LV Z L, et al. TrnL-F and ISSR Primer-based Molecular Identification and Genetic Diversity Determination on Camellia impressinervis and Three Closely Related Species [J]. Fujian Journal of Agricultural Sciences，2022，37（10）：1298−1304 doi: 10.19303/j.issn.1008-0384.2022.010.008

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TrnL-F and ISSR Primer-based Molecular Identification and Genetic Diversity Determination on Camellia impressinervis and Three Closely Related Species

doi: 10.19303/j.issn.1008-0384.2022.010.008

College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350100, China

Received Date: 2022-04-30
Rev Recd Date: 2022-07-14

Available Online: 2022-11-29

Publish Date: 2022-10-30

Abstract

Abstract

Objective Molecular identification and genetic diversity of Camellia impressinervis were studied to improve the preservation and utilization of the endangered species. Method A highly valuable ornamental and medicinal, but extremely endangered as classified by the International Union for the Conservation of Nature, C. impressinervis was compared with the closely related C. nitidissima, C. euphlebia, and C. tunghinensis. Using 19 specimens of the 4 species, validity of applying trnL-F barcode to confidently identify them was evaluated, and their genetic diversity examined based on the inter-simple sequence repeat (ISSR) primers. Result TrnL-F could accurately differentiate C. impressinervis from those closely related camellia cultivars and clustered all in a same group. The ISSR analysis grouped the 4 C. impressinervis specimens together and showed a similarity index of 0.57 with the other 3 camellia species. Conclusion The genetic fingerprint in terms of trnL-F barcode and ISSR primers successfully identified the close relationship of C. impressinervis with, but distinguishable from, C. nitidissima, C. euphlebia, and C. tunghinensis.
- Camellia,
- trnL-F,
- molecular identification,
- genetic diversity,
- ISSR

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References(42)

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