Efficiency Improvement of Low-grade Phosphorus Fertilizer by Phosphate-solubilizing Fungus JL-7

LI Xiaojun; JI Li; ZHANG Jingning; SHENG Dinghong; XIE Chengwei

doi:10.19303/j.issn.1008-0384.2022.009.017

Volume 37 Issue 9

Sep. 2022

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(9): 1237-1244

LI X J, JI L, ZHANG J N, et al. Efficiency Improvement of Low-grade Phosphorus Fertilizer by Phosphate-solubilizing Fungus JL-7 [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1237−1244 doi: 10.19303/j.issn.1008-0384.2022.009.017

Citation:

LI X J, JI L, ZHANG J N, et al. Efficiency Improvement of Low-grade Phosphorus Fertilizer by Phosphate-solubilizing Fungus JL-7 [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1237−1244 doi: 10.19303/j.issn.1008-0384.2022.009.017

Citation:

PDF( 1367 KB)

Efficiency Improvement of Low-grade Phosphorus Fertilizer by Phosphate-solubilizing Fungus JL-7

doi: 10.19303/j.issn.1008-0384.2022.009.017

School of Chemistry and Chemical Engineering, Guizhou University, Guiyang, Guizhou　550025, China

Received Date: 2022-03-05
Accepted Date: 2022-03-05
Rev Recd Date: 2022-07-22

Available Online: 2022-10-05

Publish Date: 2022-09-30

Abstract

Abstract

Objective A fungal strain highly efficient in dissociating low-grade phosphorus ore for fertilization was isolated to determine the applicability. Method Soil samples at Guizhou Tongren Tobacco Planting Base were screened using the sand culture method for fungi that exhibited capability to solubilize phosphate. The selected isolate was identified by physiological, biochemical, and molecular biological tests. Conditions for optimal phosphate solubilization were determined by single factor and orthogonal experiments. Effect of the fungal addition on fertilization was verified by a pot test conducted on tobacco seedlings. Result The selected isolate was code-named JL-7 and, subsequently, identified to be a strain of Aspergillus fumigatiaffinis. The optimized conditions to maximize the phosphate-solubilization of JL-7 applied an inoculation at the rate of 1×10⁵ cfu·mL⁻¹ with an initial pH of 6 to incubate at 26 ℃ for 8 d. The process dissolved a low-grade phosphorus ore material up to 967.4 mg·kg⁻¹ with the resulting solution reduced to approximately pH 2.9. In the pot experiment, the Yunyan 87 tobacco seedlings grown on a medium with the addition of a JL-7-inoculated phosphorus fertilizer had the stem girth, plant height, and maximum leaf area increased by 44.60%, 57.29%, and 62.90%, respectively, over control. Meanwhile, the pot soil increased 48.5% on available phosphorus, 3.7% on available potassium, and 9.1% on alkali-hydrolysable nitrogen after the planting as well. Conclusion The identified strongly phosphate-solubilizing Fungus JL-7 displayed a significant and consistent ability of dissolving phosphate. It was considered a potential candidate to be widely promoted as a microbial fertilization enhancer.
- Phosphate-dissolving fungus,
- low-grade phosphate ore,
- condition optimization,
- microbial fertilization enhancer

FullText(HTML)

References(29)

References

[1]	SHAHZAD S M, ARIF M S, RIAZ M, et al. Interaction of compost additives with phosphate solubilizing rhizobacteria improved maize production and soil biochemical properties under dryland agriculture [J]. Soil and Tillage Research, 2017, 174(12): 70−80.
[2]	VENEKLAAS E J, LAMBERS H, BRAGG J, et al. Opportunities for improving phosphorus-use efficiency in crop plants [J]. The New Phytologist, 2012, 195(2): 306−320. doi: 10.1111/j.1469-8137.2012.04190.x
[3]	TALLAPRAGADA P, SESHACHALA U. Phosphate-solubilizing microbes and their occurrence in the rhizospheres of Piper betel in Karnataka, India [J]. Turkish Journal of Biology, 2012, 36(1): 25−35.
[4]	ZHANG J, FENG L, OUYANG Y, et al. 2020. Phosphate-solubilizing bacteria and fungi in relation to phosphorus availability under different land uses for some latosols from Guangdong, China [J]. Catena, 2020, 195(5): 41−62.
[5]	池景良, 郝敏, 王志学, 等. 解磷微生物研究及应用进展 [J]. 微生物学杂志, 2021, 41(1):1−7. doi: 10.3969/j.issn.1005-7021.2021.01.001 CHI J L, HAO M, WANG Z X, et al. Advances in research and application of phosphorus-solubilizing microorganism [J]. Journal of Microbiology, 2021, 41(1): 1−7.(in Chinese) doi: 10.3969/j.issn.1005-7021.2021.01.001
[6]	王向英, 武欣, 张杰, 等. 解磷菌在复垦土壤中的定殖及促生效果研究 [J]. 东北农业大学学报, 2021, 52(7):40−47. doi: 10.3969/j.issn.1005-9369.2021.07.005 WANG X Y, WU X, ZHANG J, et al. Colonization of phosphate-solubilizing bacteria in reclaimed soil and the growth-promoting effects on maize [J]. Journal of Northeast Agricultural University, 2021, 52(7): 40−47.(in Chinese) doi: 10.3969/j.issn.1005-9369.2021.07.005
[7]	张云霞, 雷鹏, 许宗奇, 等. 一株高效解磷菌Bacillus subtilis JT-1的筛选及其对土壤微生态和小麦生长的影响 [J]. 江苏农业学报, 2016, 32(5):1073−1080. doi: 10.3969/j.issn.1000-4440.2016.05.019 ZHANG Y X, LEI P, XU Z Q, et al. Screening of a high-efficiency phosphate solubilizing bacterium Bacillus subtilis JT-1 and its effects on soil microecology and wheat growth [J]. Jiangsu Journal of Agricultural Sciences, 2016, 32(5): 1073−1080.(in Chinese) doi: 10.3969/j.issn.1000-4440.2016.05.019
[8]	林志伟, 肖翠红, 白鑫雨, 等. 解磷菌DQ-N对大豆种子萌发及苗期生长的影响 [J]. 大豆科学, 2021(5):676−681. LIN Z W, XIAO C H, BAI X Y, et al. Effects of DQ-N strain on seed germination and seedling growth of soybean [J]. Soybean Science, 2021(5): 676−681.(in Chinese)
[9]	AHMAD A, ZAFAR U, KHAN A, et al. Effectiveness of compost inoculated with phosphate solubilizing bacteria [J]. Journal of Applied Microbiology, 2022, 133(2): 1115−1129. doi: 10.1111/jam.15633
[10]	鲁如坤. 土壤磷素水平和水体环境保护 [J]. 磷肥与复肥, 2003, 18(1):4−8. doi: 10.3969/j.issn.1007-6220.2003.01.002 LU R K. The phosphorus level of soil and environmental protection of water body [J]. Phosphate & Compound Fertilizer, 2003, 18(1): 4−8.(in Chinese) doi: 10.3969/j.issn.1007-6220.2003.01.002
[11]	ANZUAY M S, CIANCIO M G R, LUDUEÑA L M, et al. Growth promotion of peanut (Arachis hypogaea L. ) and maize (Zea mays L. ) plants by single and mixed cultures of efficient phosphate solubilizing bacteria that are tolerant to abiotic stress and pesticides [J]. Microbiological Research, 2017, 199: 98−109. doi: 10.1016/j.micres.2017.03.006
[12]	唐哲, 杨洪一, 李丽丽. 解磷真菌的研究进展与应用前景 [J]. 安徽农业科学, 2014(32):11287−11288,11296. doi: 10.3969/j.issn.0517-6611.2014.32.022 TANG Z, YANG H Y, LI L L. Advance and application prospect of phosphate-solubilizing fungi [J]. Journal of Anhui Agricultural Sciences, 2014(32): 11287−11288,11296.(in Chinese) doi: 10.3969/j.issn.0517-6611.2014.32.022
[13]	赵小蓉, 林启美, 李保国. 溶磷菌对4种难溶性磷酸盐溶解能力的初步研究 [J]. 微生物学报, 2002, 42(2):236−241. doi: 10.3321/j.issn:0001-6209.2002.02.017 ZHAO X R, LIN Q M, LI B G. The solubilization of four insoluble phosphates by some microorganisms [J]. Acta Microbiologica Sinica, 2002, 42(2): 236−241.(in Chinese) doi: 10.3321/j.issn:0001-6209.2002.02.017
[14]	SHIRMOHAMMADI E, ALIKHANI H A, POURBABAEI A A, et al. Improved phosphorus (P) uptake and yield of rainfed wheat fed with P fertilizer by drought-tolerant phosphate-solubilizing fluorescent pseudomonads strains: A field study in drylands [J]. Journal of Soil Science and Plant Nutrition, 2020, 20(4): 2195−2211. doi: 10.1007/s42729-020-00287-x
[15]	刘洋, 洪坚平, 卫迎. 接种AM真菌与解磷细菌对矿区复垦土壤磷形态及油菜产量的影响 [J]. 山西农业科学, 2018(5):785−790,861. doi: 10.3969/j.issn.1002-2481.2018.05.27 LIU Y, HONG J P, WEI Y. Effects of inoculation of AM fungi and phosphate solubilizing bacteria on phosphorus morphology and rape yield in reclaimed soil in mining area [J]. Journal of Shanxi Agricultural Sciences, 2018(5): 785−790,861.(in Chinese) doi: 10.3969/j.issn.1002-2481.2018.05.27
[16]	IWASAKI S, FUKUDA M, IKAZAKI K, et al. Optimal P fertilization using low-grade phosphate rock-derived fertilizer for rice cultivation under different ground-water conditions in the Central Plateau of Burkina Faso [J]. Soil Science and Plant Nutrition, 2021, 67(4): 460−470. doi: 10.1080/00380768.2021.1932584
[17]	CALLE-CASTANEDA S M, MARQUEZ-GODOY M A, HERNANDEZ-ORTIZ J P. Phosphorus recovery from high concentrations of low-grade phosphate rocks using the biogenic acid produced by the acidophilic bacteria Acidithiobacillus thiooxidans [J]. Minerals Engineering, 2018, 115(1): 97−105.
[18]	李豆豆, 尚双华, 韩巍, 等. 一株高效解磷真菌新菌株的筛选鉴定及解磷特性 [J]. 应用生态学报, 2019, 30(7):2384−2392. doi: 10.13287/j.1001-9332.201907.033 LI D D, SHANG S H, HAN W, et al. Screening, identification, and phosphate solubilizing characteristics of a new efficient phosphate solubilizing fungus [J]. Chinese Journal of Applied Ecology, 2019, 30(7): 2384−2392.(in Chinese) doi: 10.13287/j.1001-9332.201907.033
[19]	杨锦发. 多目标生态地球化学土壤样品高精度测试与质量监控 [J]. 岩矿测试, 2007, 26(1):36−39. doi: 10.3969/j.issn.0254-5357.2007.01.010 YANG J F. High precision measurements and quantity monitoring and control for soil sample analysis in eco-geochemistry survey [J]. Rock and Mineral Analysis, 2007, 26(1): 36−39.(in Chinese) doi: 10.3969/j.issn.0254-5357.2007.01.010
[20]	马卫, 刘诚, 邵闯, 等. 一株高效溶磷真菌MEM07的筛选鉴定及其溶磷条件优化 [J]. 绿色科技, 2017(20):193−195. doi: 10.16663/j.cnki.lskj.2017.20.068 MA W, LIU C, SHAO C, et al. Screening and identification of a high-efficiency phosphate-dissolving fungus MEM07 and optimization of its phosphate-dissolving conditions [J]. Journal of Green Science and Technology, 2017(20): 193−195.(in Chinese) doi: 10.16663/j.cnki.lskj.2017.20.068
[21]	全国农业技术推广服务中心. 土壤分析技术规范[M]. 第2版. 北京: 中国农业出版社, 2006.
[22]	魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979.
[23]	柴文亚, 李红丽, 王勇, 等. 生物有机肥防治烟草黑胫病效果及对烟株生长发育的影响 [J]. 天津农业科学, 2014, 20(4):24−27. doi: 10.3969/j.issn.1006-6500.2014.04.008 CHAI W Y, LI H L, WANG Y, et al. Effect of bio-organic fertilizer on prevention and control of tobacco black shank disease and its influence on tobacco growth [J]. Tianjin Agricultural Sciences, 2014, 20(4): 24−27.(in Chinese) doi: 10.3969/j.issn.1006-6500.2014.04.008
[24]	汤宏, 李向阳, 曾掌权, 等. 不同施磷量对烟草生长及产量的影响 [J]. 华北农学报, 2018(S1):201−207. doi: 10.7668/hbnxb.2018.S1.033 TANG H, LI X Y, ZENG Z Q, et al. Effects of different phosphorus application rate on growth and yield of tobacco [J]. Acta Agriculturae Boreali-Sinica, 2018(S1): 201−207.(in Chinese) doi: 10.7668/hbnxb.2018.S1.033
[25]	李玥, 赖勇林, 王军, 等. 不同养分缺乏对烤烟根系形态及营养生长的影响 [J]. 中国烟草科学, 2015, 36(2):60−65. doi: 10.13496/j.issn.1007-5119.2015.02.011 LI Y, LAI Y L, WANG J, et al. Effect of different nutrient deficiencies on root morphology and vegetative growth in flue-cured tobacco [J]. Chinese Tobacco Science, 2015, 36(2): 60−65.(in Chinese) doi: 10.13496/j.issn.1007-5119.2015.02.011
[26]	李静, 艾加敏, 余天飞, 等. 一株溶磷真菌的鉴定及其促生特性研究 [J]. 福建农业学报, 2021(10):1224−1230. doi: 10.19303/j.issn.1008-0384.2021.10.015 LI J, AI J M, YU T F, et al. Identification and growth-promoting effect of a phosphate-solubilizing fungus on wheat seedlings [J]. Fujian Journal of Agricultural Sciences, 2021(10): 1224−1230.(in Chinese) doi: 10.19303/j.issn.1008-0384.2021.10.015
[27]	ALIYAT F Z, MALDANI M, EL GUILLI M, et al. Phosphate-solubilizing bacteria isolated from phosphate solid sludge and their ability to solubilize three inorganic phosphate forms: Calcium, iron, and aluminum phosphates [J]. Microorganisms, 2022, 10(5): 980. doi: 10.3390/microorganisms10050980
[28]	MONROY MIGUEL R, CARRILLO GONZÁLEZ R, RIOS LEAL E, et al. Screening bacterial phosphate solubilization with bulk-tricalcium phosphate and hydroxyapatite nanoparticles [J]. Antonie Van Leeuwenhoek, 2020, 113(7): 1033−1047. doi: 10.1007/s10482-020-01409-2
[29]	刘娟, 张乃明, 何云. 黑曲霉素J4对中低品位磷矿粉的溶磷效果及重金属释放的影响 [J]. 生态环境学报, 2020, 29(6):1260−1267. doi: 10.16258/j.cnki.1674-5906.2020.06.023 LIU J, ZHANG N M, HE Y. Effect of Niger Aspergillus J4 on phosphate dissolution and release of heavy metals in medium and low grade phosphate powder [J]. Ecology and Environment Sciences, 2020, 29(6): 1260−1267.(in Chinese) doi: 10.16258/j.cnki.1674-5906.2020.06.023