Pathogens of Tomato Damping-off Disease in Yinchuan, Ningxia

YANG Hong; WANG Xiaomin; SONG Lina; LIU Wenjuan; LI Liping; CHENG Guoxin; GUO Meng; FU Jinjun; HU Xinhua

doi:10.19303/j.issn.1008-0384.2022.009.013

Volume 37 Issue 9

Sep. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(9): 1209-1215

YANG H, WANG X M, SONG L N, et al. Pathogens of Tomato Damping-off Disease in Yinchuan, Ningxia [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1209−1215 doi: 10.19303/j.issn.1008-0384.2022.009.013

Citation:

YANG H, WANG X M, SONG L N, et al. Pathogens of Tomato Damping-off Disease in Yinchuan, Ningxia [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1209−1215 doi: 10.19303/j.issn.1008-0384.2022.009.013

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Pathogens of Tomato Damping-off Disease in Yinchuan, Ningxia

doi: 10.19303/j.issn.1008-0384.2022.009.013

YANG Hong^1
,,
WANG Xiaomin^{1, 2, 3, 4
,
,},
SONG Lina¹,
LIU Wenjuan¹,
LI Liping¹,
CHENG Guoxin^{1, 2, 3, 4},
GUO Meng^{1, 2, 3, 4},
FU Jinjun⁵,
HU Xinhua⁵

1.
School of Agriculture, Ningxia University, Yinchuan, Ningxia　750021, China
2.
Ningxia Modern Facility Horticulture Engineering Technology Research Center, Yinchuan, Ningxia　750021, China
3.
Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Yinchuan, Ningxia　750021, China
4.
Ningxia Facility Horticulture (Ningxia University) Technology Innovation Center, Yinchuan, Ningxia　750021, China
5.
Ningxia Jufeng Seedlings Co., Ltd.， Yinchuan, Ningxia　750021，China

Received Date: 2022-03-02
Rev Recd Date: 2022-05-09

Available Online: 2022-08-29

Publish Date: 2022-09-30

Abstract

Abstract

Objective To find out the pathogen of tomato damping-off in Yinchuan area of Ningxia, and lay a foundation for disease control and disease resistance breeding. Methods Possible pathogenic microbes of the disease on tomatoes were isolated by the conventional tissue isolation method and identified based on their morphology, pathogenicity, and rDNA-ITS sequence. Result Of the two suspected species, Pathogen No. 1 had oval or nearly spherical sporangia with papillae and long stalks. It was preliminarily determined as Phytophthora capsici. The spherical ovipositor of Pathogen No. 2 had straight stalks with spherical, smooth oospores that did not fully fill the sacs and the wide rod- or lemon-like-shaped male organ grew laterally. It was presumed to be Pythium aphanidermatum. After inoculation on tomato cotyledons the inoculation of either of the two isolates caused the seedling stem constriction and plant collapse showing the typical symptoms of dark brown spots at infected sites. The phylogenetic trees constructed based on the ITS sequences of the two isolates confirmed No. 1 to belong in the branch of P. capsici, and No. 2 of P. aphanidermatum. Conclusion The pathogens that caused damping-off on tomato plants in Yinchuan were positively identified to be strains of P. capsici and P. aphanidermatum.
- Tomato,
- Yinchuan area,
- damping-off disease,
- pathogen,
- identification

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References(34)

References

[1]	叶玉龙. 18份国外番茄种质资源主要性状研究[D]. 贵阳: 贵州大学, 2007. YE Y L. A study on main characteristics of 18 foreign tomato germplasm resources[D]. Guiyang: Guizhou University, 2007. (in Chinese)
[2]	杨冬艳, 桑婷, 王丹, 等. 日光温室纸膜覆盖下不同种植模式对番茄产量及根系分布的影响 [J]. 北方园艺, 2022(3):64−70. YANG D Y, SANG T, WANG D, et al. Effects of different planting pattern on tomato yield and root distribution under paper film mulching in solar greenhouse [J]. Northern Horticulture, 2022(3): 64−70.(in Chinese)
[3]	银玲, 京山幸二, 田迅, 等. 内蒙古地区菠菜猝倒病病原菌鉴定 [J]. 中国蔬菜, 2016(11):50−55. YIN L, KOJI K, TIAN X, et al. Identification of spinach damping-off pathogens in Inner Mongolia Region [J]. China Vegetables, 2016(11): 50−55.(in Chinese)
[4]	何美文. 番茄种子抗猝倒病包衣配方筛选、安全性评价与防效研究[D]. 杭州: 浙江农林大学, 2014. HE M W. The screening and safety evaluation of the fungicide seed coating formula and its effects on damping-off in tomato seedlings[D]. Hangzhou: Zhejiang A & F University, 2014. (in Chinese)
[5]	BARCHENGER D W, LAMOUR K H, BOSLAND P W. Challenges and strategies for breeding resistance in Capsicum annuum to the multifarious pathogen, Phytophthora capsici [J]. Frontiers in Plant Science, 2018, 9: 628. doi: 10.3389/fpls.2018.00628
[6]	LAMOUR K H, STAM R, JUPE J, et al. The oomycete broad-host-range pathogen Phytophthora capsici [J]. Molecular Plant Pathology, 2012, 13(4): 329−337. doi: 10.1111/j.1364-3703.2011.00754.x
[7]	刘婷婷. 我国不同地区辣椒疫霉群体遗传结构和抗药性分析[D]. 扬州: 扬州大学, 2016. LIU T T. Genetic structure and drug resistance of Phytophthora capsici populations in different regions of China[D]. Yangzhou: Yangzhou University, 2016. (in Chinese)
[8]	KAGEYAMA K, SUZUKI M, PRIYATMOJO A, et al. Characterization and identification of asexual strains of Pythium associated with root rot of Rose in Japan [J]. Phytopathology, 2010, 151(9): 485−491.
[9]	司越, 司凤举, 吴仁锋. 蔬菜苗期猝倒病和立枯病的识别与防治 [J]. 长江蔬菜, 2005(1):31−58. doi: 10.3865/j.issn.1001-3547.2005.01.024 SI Y, SI F J, WU R F. Identification and control of damping-off and damping-off of vegetables at seedling stage [J]. Journal of Changjiang Vegetables, 2005(1): 31−58.(in Chinese) doi: 10.3865/j.issn.1001-3547.2005.01.024
[10]	陈志敏, 吴昊鑫, 曾慧芳, 等. 烟田瓜果腐霉的分离鉴定及其拮抗木霉菌株的筛选 [J]. 福建农林大学学报(自然科学版), 2009, 38(1):11−15. doi: 10.13323/j.cnki.j.fafu(nat.sci.).2009.01.007 CHEN Z M, WU H X, ZENG H F, et al. Identification of Pythium aphanidermatum isolated from tobacco field and the screening of Trichoderma spp. for antagonistic agent [J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2009, 38(1): 11−15.(in Chinese) doi: 10.13323/j.cnki.j.fafu(nat.sci.).2009.01.007
[11]	周黎, 李国英, 丁建军. 新疆加工番茄根腐病病原的分离和鉴定 [J]. 新疆农业科学, 2008, 45(1):130−134. ZHOU L, LI G Y, DING J J. Isolation and identification of pathogen of root-rot disease processing tomato in Xinjiang [J]. Xinjiang Agricultural Sciences, 2008, 45(1): 130−134.(in Chinese)
[12]	毕艳红, 慕卫, 刘峰, 等. 杀菌剂对辣椒疫霉菌的毒力及其所致番茄猝倒病的控制效果研究 [J]. 农药学学报, 2004, 6(4):43−47. doi: 10.3321/j.issn:1008-7303.2004.04.009 BI Y H, MU W, LIU F, et al. The control effect and toxicity of fungicides on tomato seedling damping-off caused by Phytophthora capsici leonian [J]. Chinese Journal of Pesticide Science, 2004, 6(4): 43−47.(in Chinese) doi: 10.3321/j.issn:1008-7303.2004.04.009
[13]	SCHOCH C L, SEIFERT K A, HUHNDORF S, et al. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi [J]. PNAS, 2012, 109(16): 6241−6246. doi: 10.1073/pnas.1117018109
[14]	方中达. 植病研究方法[M]. 北京: 农业出版社, 1979.
[15]	孙银银. 西北部分地区致病疫霉菌的交配型分布和遗传多样性初步分析[D]. 杨凌: 西北农林科技大学, 2010. SUN Y Y. Preliminary analysis of mating type distribution and genetic diversity of Phytophthora infestans in northwestern china[D]. Yangling: Northwest A & F University, 2010. (in Chinese)
[16]	郑小波. 疫霉菌及其研究技术[M]. 北京: 中国农业出版社, 1997.
[17]	邹芬, 何烈干, 李湘民, 等. 黄瓜绵腐病病原菌鉴定及防治药剂的筛选 [J]. 植物保护学报, 2020, 47(6):1313−1320. doi: 10.13802/j.cnki.zwbhxb.2020.2020011 ZOU F, HE L G, LI X M, et al. Identification of the pathogen causing fruit rot on cucumber and screening of fungicides [J]. Journal of Plant Protection, 2020, 47(6): 1313−1320.(in Chinese) doi: 10.13802/j.cnki.zwbhxb.2020.2020011
[18]	王贤, 王剑英. 番茄夏季穴盘育苗技术 [J]. 现代农村科技, 2021(11):26. doi: 10.3969/j.issn.1674-5329.2021.11.020 WANG X, WANG J Y. Plug seedling technique of tomato in summer [J]. Modern Rural Science and Technology, 2021(11): 26.(in Chinese) doi: 10.3969/j.issn.1674-5329.2021.11.020
[19]	赵思峰, 方许阳, 姜海荣, 等. 新疆加工番茄腐霉根腐病病原鉴定及其rDNA的ITS区段分析 [J]. 植物保护学报, 2009, 36(3):219−224. doi: 10.3321/j.issn:0577-7518.2009.03.005 ZHAO S F, FANG X Y, JIANG H R, et al. Identification and rDNA ITS sequence analysis of the Pythium root rot pathogens of processing tomato in Xinjiang [J]. Journal of Plant Protection, 2009, 36(3): 219−224.(in Chinese) doi: 10.3321/j.issn:0577-7518.2009.03.005
[20]	周黎. 新疆加工番茄根腐病的病原鉴定和防治[D]. 石河子: 石河子大学, 2007. ZHOU L. The research of processing tomato root rot identification and prevention in xinjiang[D]. Shihezi: Shihezi University, 2007. (in Chinese)
[21]	李园. 土壤消毒及土传病原真菌的分子生物学鉴定[D]. 北京: 中国农业科学院, 2006. LI Y. Soil disinfestations and molecular biology on identification of soilborne fungi[D]. Beijing: Chinese Academy of Agricultural Sciences, 2006. (in Chinese)
[22]	蒋盛岩, 张志光. 真菌的分子生物学鉴定方法研究进展 [J]. 生物学通报, 2002, 37(10):4−6. doi: 10.3969/j.issn.0006-3193.2002.10.002 JIANG S Y, ZHANG Z G. Research progress of molecular biological identification methods of fungi based on [J]. Bulletin of Biology, 2002, 37(10): 4−6.(in Chinese) doi: 10.3969/j.issn.0006-3193.2002.10.002
[23]	VOLOSSIOUK T, ROBB E J, NAZAR R N. Direct DNA extraction for PCR-mediated assays of soil organisms [J]. Appl Environ Microbiol, 1995, 61(11): 3972−3976. doi: 10.1128/aem.61.11.3972-3976.1995
[24]	陆杰, 王珣, 窦道龙, 等. 黑龙江省籽用南瓜疫病病原菌致病力分析与抗性种质资源评价 [J]. 植物病理学报, 2021, 51(1):49−58. LU J, WANG X, DOU D L, et al. The occurrence of Phytophthora blight, pathogenicity identification of isolates germplasm resistance evaluation of seed-used pumpkin in Heilongjiang Province [J]. Acta Phytopathologica Sinica, 2021, 51(1): 49−58.(in Chinese)
[25]	席亚东, 乔昕, 房超, 等. 四川茄子对绵疫病菌Phytophthora capsici的抗性鉴定与评价 [J]. 北方园艺, 2020(14):8−17. XI Y D, QIAO X, FANG C, et al. Sichuan's eggplant resistance to Phytophthora blight pathogen of Phytophthora capsici [J]. Northern Horticulture, 2020(14): 8−17.(in Chinese)
[26]	卢凯, 齐军山, 祁凯, 等. 大豆根腐病腐霉病原鉴定 [J]. 中国油料作物学报, 2022, 44(3):652−658. doi: 10.19802/j.issn.1007-9084.2021064 LU K, QI J S, QI K, et al. Pathogen identification of Pythium root rot disease on soybean [J]. Chinese Journal of Oil Crop Sciences, 2022, 44(3): 652−658.(in Chinese) doi: 10.19802/j.issn.1007-9084.2021064
[27]	邹芬, 何烈干, 赖金美, 等. 江西省丝瓜果实腐烂病病原及寄主范围研究 [J]. 江苏农业科学, 2021, 49(14):90−94. doi: 10.15889/j.issn.1002-1302.2021.14.017 ZOU F, HE L G, LAI J M, et al. Study on pathogen and host range of loofah fruit rot disease in Jiangxi Province [J]. Jiangsu Agricultural Sciences, 2021, 49(14): 90−94.(in Chinese) doi: 10.15889/j.issn.1002-1302.2021.14.017
[28]	洪小雨, 李敏, 弓德强, 等. 豇豆贮藏期软腐病病原菌鉴定及其生物学特性研究 [J]. 热带作物学报, 2021, 42(4):1099−1105. doi: 10.3969/j.issn.1000-2561.2021.04.028 HONG X Y, LI M, GONG D Q, et al. Identification and biological characteristics of the pathogen causing postharvest decay of cowpea [J]. Chinese Journal of Tropical Crops, 2021, 42(4): 1099−1105.(in Chinese) doi: 10.3969/j.issn.1000-2561.2021.04.028
[29]	李发福. 甜瓜猝倒病的发生规律与防治措施 [J]. 园艺与种苗, 2021, 41(9):30−31,47. doi: 10.16530/j.cnki.cn21-1574/s.2021.09.012 LI F F. Occurrence regularity and control measures of muskmelon damping-off [J]. Horticulture & Seed, 2021, 41(9): 30−31,47.(in Chinese) doi: 10.16530/j.cnki.cn21-1574/s.2021.09.012
[30]	何烈干, 邹芬, 李湘民, 等. 江西省番茄绵疫病菌对嘧菌酯的敏感性检测及抗性风险分析 [J]. 农药学学报, 2021, 23(1):117−123. doi: 10.16801/j.issn.1008-7303.2021.0031 HE L G, ZOU F, LI X M, et al. Sensitivity detection and resistance risk analysis of Phytophthora capsici on tomato to azoxystrobin in Jiangxi Province [J]. Chinese Journal of Pesticide Science, 2021, 23(1): 117−123.(in Chinese) doi: 10.16801/j.issn.1008-7303.2021.0031
[31]	朱辉, 王满意, 李宝聚, 等. 辣椒根腐型疫病病原鉴定及防治药剂筛选 [J]. 植物保护学报, 2007, 34(4):373−378. doi: 10.3321/j.issn:0577-7518.2007.04.008 ZHU H, WANG M Y, LI B J, et al. Screening of fungicide and identification of capsicum phytopathora root rot [J]. Journal of Plant Protection, 2007, 34(4): 373−378.(in Chinese) doi: 10.3321/j.issn:0577-7518.2007.04.008
[32]	任爱芝, 赵培宝, 李营, 等. 保护地番茄茎基腐病拮抗真菌的分离与筛选 [J]. 安徽农业科学, 2009, 37(1):213−214. doi: 10.3969/j.issn.0517-6611.2009.01.095 REN A Z, ZHAO P B, LI Y, et al. Isolating and screening of antagonistic fungi against root-stem rotten diseasel of tomato in green house [J]. Journal of Anhui Agricultural Sciences, 2009, 37(1): 213−214.(in Chinese) doi: 10.3969/j.issn.0517-6611.2009.01.095
[33]	毕艳红. 光照和温度因素对番茄猝倒病发生的影响及药剂调控[D]. 泰安: 山东农业大学, . BI Y H. The impact of luminous intensity and temperature on the incidence of tomato seedling damping-off and control by fungicides and PGRs[D]. Taian: Shandong Agricultural University, . (in Chinese)
[34]	王宽仓, 查先芳, 马国忠, 等. 宁夏腐霉种类及其对主要蔬菜致病性的研究 [J]. 云南农业大学学报, 1992, 7(4):206−210. WANG K C, ZHA X F, MA G Z, et al. Studies on Pythium and the pathogenicity to the principal vegetables in Ningxia [J]. Journal of Yunnan Agricultural University, 1992, 7(4): 206−210.(in Chinese)