Construction of Yeast Two-hybrid cDNA Library on Tomato Spotted Wilt Tospovirus-infected<i> Frankliniella occidentalis</i>

YE Qian; LU Bingxin; WEI Yan; CHEN Jianbin; LIU Yong; ZHANG Songbai; ZHANG Songbai; ZHENG Limin

doi:10.19303/j.issn.1008-0384.2022.009.012

Volume 37 Issue 9

Sep. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(9): 1203-1208

YE Q, LU B X, WEI Y, et al. Construction of Yeast Two-hybrid cDNA Library on Tomato Spotted Wilt Tospovirus-infected Frankliniella occidentalis [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1203−1208 doi: 10.19303/j.issn.1008-0384.2022.009.012

Citation:

YE Q, LU B X, WEI Y, et al. Construction of Yeast Two-hybrid cDNA Library on Tomato Spotted Wilt Tospovirus-infected Frankliniella occidentalis [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1203−1208 doi: 10.19303/j.issn.1008-0384.2022.009.012

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Construction of Yeast Two-hybrid cDNA Library on Tomato Spotted Wilt Tospovirus-infected Frankliniella occidentalis

doi: 10.19303/j.issn.1008-0384.2022.009.012

YE Qian^{1, 2
,},
LU Bingxin²,
WEI Yan^{1, 2},
CHEN Jianbin²,
LIU Yong²,
ZHANG Songbai²,
ZHANG Songbai^{1
,
,},
ZHENG Limin^{2
,
,}

1.
Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou, Hubei　434025, China
2.
Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, Hunan　410125, China

Received Date: 2022-06-26
Accepted Date: 2022-06-26
Rev Recd Date: 2022-08-18

Available Online: 2022-10-05

Publish Date: 2022-09-30

Abstract

Abstract

Objective A two-hybrid eDNA library was constructed for studying the interaction between tomato spotted wilt tospovirus (TSWV) and Frankliniella occidentalis to decipher the mechanism of the disease transmission by thrip and the factors involved in the virus infection. Method A highly infected F. occidentalis population was created by inoculating TSWV into the thrip nymphs. Thereby, a yeast two-hybrid three-frame cDNA library on TSWV-infected F. occidentalis was constructed using the SMART technology. Result The established 3 reading frame cDNA libraries had the capacities of 3.0×10⁶, 2.0×10⁶, and 2.0×10⁶ cfu. The actual amplification base of the library was more than 1.5×10⁶ cfu or 5 ×10⁵ cfu each with an average insert fragment of 0.5–3.0 kb in length. From the library 16 clones were randomly selected, sequenced, and compared with the GenBank database for of homology verification on the insert fragments. Conclusion The established cDNA library had an ample capacity and high recombination rate adequate for studies on the interactions between TSWV and F. occidentalis.
- Tomato spotted wilt tospovirus,
- Frankliniella occidentalis,
- yeast two-hybrid cDNA library

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References(24)

References

[1]	BRØDSGAARD H F. Cold hardiness and tolerance to submergence in water in Frankliniella occidentalis (Thysanoptera: Thripidae) [J]. Environmental Entomology, 1993, 22(3): 647−653. doi: 10.1093/ee/22.3.647
[2]	KIRK W D J, TERRY L I. The spread of the western flower thrips Frankliniella occidentalis (Pergande) [J]. Agricultural and Forest Entomology, 2003, 5(4): 301−310. doi: 10.1046/j.1461-9563.2003.00192.x
[3]	陈建斌, 刘勇, 郑立敏, 等. 番茄斑萎病毒及其与传播介体西花蓟马互作的研究进展 [J]. 贵州农业科学, 2017(4):54−58. CHEN J B, LIU Y, ZHENG L M, et al. Advances in tomato spotted wilt virus and interaction of its vector frankliniellaoccidentalis [J]. Guizhou Agricultural Sciences, 2017(4): 54−58.(in Chinese)
[4]	张友军, 吴青君, 徐宝云, 等. 危险性外来入侵生物——西花蓟马在北京发生危害 [J]. 植物保护, 2003, 29(4):58−59. ZHANG Y J, WU Q J, XU B Y, et al. Dangerous invasive pest Frankliniella occidentalis occurred in Beijing [J]. Plant Protection., 2003, 29(4): 58−59.(in Chinese)
[5]	REITZ S R. Biology and ecology of the western flower thrips (Thysanoptera: Thripidae): the making of a pest [J]. Florida Entomologist, 2009, 92(1): 7−13. doi: 10.1653/024.092.0102
[6]	吕要斌, 贝亚维, 林文彩, 等. 西花蓟马的生物学特性、寄主范围及危害特点 [J]. 浙江农业学报, 2004(5):317−320. LU Y B, BEI Y W, LIN W C, et al. The biology, host and damage of western flower Thrips, Frankliniella occidentalis (Pergande) [J]. Acta Agriculturae Zhejiangensis, 2004(5): 317−320.(in Chinese)
[7]	PARRELLA G, GOGNALONS P, GÉBRÉ-SÉLASSIÉ K, et al. An update of the host range of Tomato Spotted Wilt Virus [J]. Journal of Plant Pathology, 2003, 85(4): 227−264.
[8]	WHITFIELD A E, ULLMAN D E, GERMAN T L. Tomato spotted wilt virus glycoprotein G(C) is cleaved at acidic pH [J]. Virus Research, 2005, 110(1/2): 183−186.
[9]	WETERING, DE V, GOLDBACH R, et al. Tomato spotted wilt tospovirus ingestion by first instar larvae of Frankliniella occidentalis is a prerequisite for transmission [J]. Phytopathology, 1996, 86(9): 900−905. doi: 10.1094/Phyto-86-900
[10]	MONTERO-ASTÚA M, ULLMAN D E, WHITFIELD A E. Salivary gland morphology, tissue tropism and the progression of Tospovirus infection in Frankliniella occidentalis [J]. Virology, 2016, 493: 39−51. doi: 10.1016/j.virol.2016.03.003
[11]	SCHNEWEIS D J, WHITFIELD A E, ROTENBERG D. Thrips developmental stage-specific transcriptome response to tomato spotted wilt virus during the virus infection cycle in Frankliniella occidentalis, the primary vector [J]. Virology, 2017, 500: 226−237. doi: 10.1016/j.virol.2016.10.009
[12]	史晓斌, 谢文, 张友军. 植物病毒病媒介昆虫的传毒特性和机制研究进展 [J]. 昆虫学报, 2012, 55(7):841−848. SHI X B, XIE W, ZHANG Y J. Advances in the characteristics and mechanisms of the transmission of plant viruses by insect vectors [J]. Science China Earth Sciences, 2012, 55(7): 841−848.(in Chinese)
[13]	傅意茗, 陈婵珊, 黄芳, 等. 植物病毒与媒介昆虫互作促进其传播的研究进展 [J]. 植物保护学报, 2022(3):711−720. FU Y M, CHEN C S, HUANG F, et al. Advances in the transmission of plant viruses promoted by the interaction between the viruses and insect vectors [J]. Journal of Plant Protection, 2022(3): 711−720.(in Chinese)
[14]	BADILLO-VARGAS I E, CHEN Y, MARTIN K M, et al. Discovery of novel Thrips vector proteins that bind to the viral attachment protein of the plant bunyavirus tomato spotted wilt virus [J]. Journal of Virology, 2019, 93(21): e00699−e00619.
[15]	MARGARIA P, BOSCO L, VALLINO M, et al. The NSs protein of tomato spotted wilt virus is required for persistent infection and transmission by Frankliniella occidentalis [J]. Journal of Virology, 2014, 88(10): 5788−5802. doi: 10.1128/JVI.00079-14
[16]	FUJIWARA H, ISHIKAWA H. Molecular mechanism of introduction of the hidden break into the 28S rRNA of insects: Implication based on structural studies [J]. Nucleic Acids Research, 1986, 14(16): 6393−6401. doi: 10.1093/nar/14.16.6393
[17]	LIN J T, PRAMANIK J, WANG C R, et al. Study on construction of cDNA library of the treated changliver cell and quality analysis [J]. Indian Journal of Clinical Biochemistry, 2004, 19(2): 181−183. doi: 10.1007/BF02894282
[18]	刘露露, 曲俊杰, 郭泽西, 等. 霜霉菌侵染后葡萄叶片酵母双杂交cDNA文库构建 [J]. 南方农业学报, 2020(4):829−835. LIU L L, QU J J, GUO Z X, et al. Construction of a yeast two-hybrid cDNA library from Vitis vinifera leaves infected by downy mildew [J]. Journal of Southern Agriculture, 2020(4): 829−835.(in Chinese)
[19]	雷龑, 谢倩, 陈婷, 等. 炭疽菌侵染后刺葡萄果皮酵母双杂交cDNA文库构建 [J]. 福建农业学报, 2020, 35(12):1330−1335. LEI Y, XIE Q, CHEN T, et al. Yeast Two-hybrid cDNA Library Constructed from Vitis davidii Föex Pericarps Infected by Grape Ripe Rot Pathogen, Colletotrichum viniferum [J]. Fujian Journal of Agricultural Sciences, 2020, 35(12): 1330−1335.(in Chinese)
[20]	李珣, 陈思宇, 胡传活, 等. PK-15细胞酵母双杂交三框cDNA文库构建及鉴定 [J]. 南方农业学报, 2016(5):726−730. LI X, CHEN S Y, HU C H, et al. Construction and identification of yeast two-hybrid three-frame cDNA library of PK-15 cells [J]. Journal of Southern Agriculture, 2016(5): 726−730.(in Chinese)
[21]	YANG F, LEI Y Y, ZHOU M L, et al. Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system [J]. Nucleic Acids Research, 2017, 46(3): e17.
[22]	MAR T, LIU W, WANG X. Proteomic analysis of interaction between P7-1 of Southern rice black-streaked dwarf virus and the insect vector reveals diverse insect proteins involved in successful transmission [J]. J Proteomics, 2014, 102: 83−97. doi: 10.1016/j.jprot.2014.03.004
[23]	RANA V S, POPLI S, SAURAV G K, et al. A Bemisia tabaci midgut protein interacts with begomoviruses and plays a role in virus transmission [J]. Cellular Microbiology, 2016, 18(5): 663−678. doi: 10.1111/cmi.12538
[24]	OHNESORGE S, BEJARANO E R. Begomovirus coat protein interacts with a small heat-shock protein of its transmission vector (Bemisia tabaci) [J]. Insect Molecular Biology, 2009, 18(6): 693−703. doi: 10.1111/j.1365-2583.2009.00906.x