Optimizing Fermentation of Biocontrol Bacterium <i>Bacillus cereus</i> BCCY-22

ZHAO Yue; YANG Ya’nan; LI Yahua; ZHAO Honghai; XIAN Hongquan

doi:10.19303/j.issn.1008-0384.2022.007.015

Volume 37 Issue 7

Jul. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(7): 938-945

ZHAO Y, YANG Y, ZHAO H H, et al. Optimizing Fermentation of Biocontrol Bacterium Bacillus cereus BCCY-22 [J]. Fujian Journal of Agricultural Sciences，2022，37（7）：938−945 doi: 10.19303/j.issn.1008-0384.2022.007.015

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ZHAO Y, YANG Y, ZHAO H H, et al. Optimizing Fermentation of Biocontrol Bacterium Bacillus cereus BCCY-22 [J]. Fujian Journal of Agricultural Sciences，2022，37（7）：938−945 doi: 10.19303/j.issn.1008-0384.2022.007.015

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Optimizing Fermentation of Biocontrol Bacterium Bacillus cereus BCCY-22

doi: 10.19303/j.issn.1008-0384.2022.007.015

Life Science College, Shandong Qingdao Agricultural University, Qingdao, Shandong　266000, China

Received Date: 2022-02-14
Rev Recd Date: 2022-04-15

Available Online: 2022-06-20

Publish Date: 2022-07-28

Abstract

Abstract

Objective Conditions for optimal fermentation of Bacillus cereus BCCY-22, which is known to be capable of effectively controlling a variety of nematode diseases, were determined. Methods Using OD₆₀₀ of the fermentation broth as the indicator, optimal culture conditions for the process were determined with a single factor and response surface experimentation. Results High temperature, low initial pH, and restricted oxygen supply significantly governed the bacterial growth. The finalized process was carried out at 21.5 ℃ with an initial pH at 7.3 and inoculation of the seeding broth at 3% to fill 23% in a flask for 36 h. Conclusion The optimization shortened the fermentation time by 6 h and increased the biomass by 135.94%. The improvements materially provided the basis for industrialized utilization of B. cereus BCCY-22 as a biocontrol agent.
- Single factor optimization,
- Bacillus cereus,
- response surface methodology,
- culturing conditions

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References(28)

References

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