Cloning and Expression of a Thioredoxin Gene <i>CsTRXh</i>1 from <i>Citrus sinensis</i>

WANG Tao; YANG Cheng; YAN Yana; WANG Yuxiao; LI Ruimin

doi:10.19303/j.issn.1008-0384.2022.007.008

Volume 37 Issue 7

Jul. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(7): 880-885

WANG T, YANG C, YAN Y N, et al. Cloning and Expression of a Thioredoxin Gene CsTRXh1 from Citrus sinensis [J]. Fujian Journal of Agricultural Sciences，2022，37（7）：880−885 doi: 10.19303/j.issn.1008-0384.2022.007.008

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WANG T, YANG C, YAN Y N, et al. Cloning and Expression of a Thioredoxin Gene CsTRXh1 from Citrus sinensis [J]. Fujian Journal of Agricultural Sciences，2022，37（7）：880−885 doi: 10.19303/j.issn.1008-0384.2022.007.008

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Cloning and Expression of a Thioredoxin Gene CsTRXh1 from Citrus sinensis

doi: 10.19303/j.issn.1008-0384.2022.007.008

College of Life Science, Gannan Normal University, Ganzhou, Jiangxi　341000, China

Received Date: 2022-04-11
Rev Recd Date: 2022-05-26

Available Online: 2022-08-29

Publish Date: 2022-07-28

Abstract

Abstract

Objective A thioredoxin gene, CsTRXh1, was cloned from Citrus sinensis to determine the expression and subcellular localization for studying its function and role played in stress response. Method CsTRXh1 from the homology sequence in the genome of C. sinensis was cloned. Homology, phylogenetic relationship, and physicochemical properties of the gene were analyzed, and expressions in healthy and Huanglongbing-infected trees detected. The transient expression of the gene in tobacco leaves was used to find its subcellular localization. Result The successfully cloned CsTRXh1 was assigned with the GenBank accession ON125405. A multi-protein sequences alignment showed that it contained a conserved thioredoxin domain with a high similarity with other plant thioredoxin proteins. The phylogenetic analysis indicated it to be an h-type thioredoxin protein. CsTRXh1 was upregulated in the leaves of Huanglongbing-infected trees and localized in the cytoplasm and cytomembrane. Conclusion The expression induced in the Huanglongbing-infected trees revealed a possible association of CsTRXh1 with the physiological and biochemical regulations of insect-disseminated Candidatus Liberibacter asiaticus that causes the destructive, incurable citrus greening disease.
- Citrus sinensis,
- thioredoxin,
- gene cloning,
- expression analysis,
- subcellular localization

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References(21)

References

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