Interaction Identification between <i>Magnaporthe oryzae</i> Avirulence Effector Avr-PikD and Rice Protein OsDjA9

GUO Jiayuan; HONG Yonghe; HUANG Jianqiang; WU Yiling; WANG Zonghua; CHEN Songbiao; CHEN Xiaofeng

doi:10.19303/j.issn.1008-0384.2022.005.015

Volume 37 Issue 5

May 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(5): 668-674

GUO J Y, HONG Y H, HUANG J Q, et al. Interaction Identification between Magnaporthe oryzae Avirulence Effector Avr-PikD and Rice Protein OsDjA9 [J]. Fujian Journal of Agricultural Sciences，2022，37（5）：668−674 doi: 10.19303/j.issn.1008-0384.2022.005.015

Citation:

GUO J Y, HONG Y H, HUANG J Q, et al. Interaction Identification between Magnaporthe oryzae Avirulence Effector Avr-PikD and Rice Protein OsDjA9 [J]. Fujian Journal of Agricultural Sciences，2022，37（5）：668−674 doi: 10.19303/j.issn.1008-0384.2022.005.015

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Interaction Identification between Magnaporthe oryzae Avirulence Effector Avr-PikD and Rice Protein OsDjA9

doi: 10.19303/j.issn.1008-0384.2022.005.015

GUO Jiayuan^{1, 2
,},
HONG Yonghe²,
HUANG Jianqiang²,
WU Yiling²,
WANG Zonghua^{1, 2},
CHEN Songbiao^{1
,
,},
CHEN Xiaofeng^{1
,
,}

1.
College of Geography and Oceanography, Minjiang University, Fuzhou, Fujian　350108, China
2.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2022-03-31
Accepted Date: 2022-03-31
Rev Recd Date: 2022-04-11

Available Online: 2022-05-21

Publish Date: 2022-05-28

Abstract

Abstract

Objective To obtain the rice target of Magnaporthe oryzae avirulence effector Avr-PikD, the interaction between Avr-PikD and OsDjA9, one of the candidate interacting proteins screened out from a rice cDNA library, was identified. Method The interaction between Avr-PikD and OsDjA9 was verified by assays of yeast two-hybrid, pull-down, Co-IP, and luciferase complementation imaging along with co-localization analysis in the rice protoplasts. Specific domain in OsDjA9 involved in the interaction was determined by yeast two-hybrid assay. Result Avr-PikD interacts with OsDjA9 in vitro as well as in vivo. The DnaJ domain of OsDjA9 is essential for the interaction. Conclusion During the process of M. oryzae infecting rice, OsDjA9 is the target of the avirulence effector Avr-PikD secreted by the pathogen.
- Magnaporthe oryzae,
- avirulence effector Avr-PikD,
- rice,
- OsDjA9,
- protein interaction

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References(23)

References

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