Interaction Identification between Magnaporthe oryzae Avirulence Effector Avr-PikD and Rice Protein OsDjA9

  Objective  To obtain the rice target of Magnaporthe oryzae avirulence effector Avr-PikD, the interaction between Avr-PikD and OsDjA9, one of the candidate interacting proteins screened out from a rice cDNA library, was identified.

  Method   The interaction between Avr-PikD and OsDjA9 was verified by assays of yeast two-hybrid, pull-down, Co-IP, and luciferase complementation imaging along with co-localization analysis in the rice protoplasts. Specific domain in OsDjA9 involved in the interaction was determined by yeast two-hybrid assay.

  Result  Avr-PikD interacts with OsDjA9 in vitro as well as in vivo. The DnaJ domain of OsDjA9 is essential for the interaction.

  Conclusion  During the process of M. oryzae infecting rice, OsDjA9 is the target of the avirulence effector Avr-PikD secreted by the pathogen.